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Strain Species Genotype
AGD397 C. elegans aak-1(tm1944) III; aak-2(ok524) X; uthEx202. Show Description
uthEx202 [crtc-1p::crtc-1 cDNA::tdTomato::unc-54 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. Reference: Mair W, et al. Nature. 2011 Feb 17;470(7334):404-8.
MAH28 C. elegans aak-2(ok524) X; adIs2122. Show Description
adIs2122 [lgg-1::GFP + rol-6(su1006)]. Rollers. Reference: Egan DF, et al. Science. 2011 Jan 28;331(6016):456-61.
MIR13 C. elegans sir-2.1(ok434) IV; aak-2(ok524) X. Show Description
Slow growing. Maintain under normal conditions. Derived from parental strains RB754 [aak-2(ok524)] and VC199 [sir-2.1(ok434)]. Reference: Schmeisser S, et al. Molecular Metabolism February 15, 2013. DOI: 10.1016/j.molmet.2013.02.002.
PE863 C. elegans feIs4 V; aak-2(ok524) X. Show Description
feIs4 [sur-5p::luciferase::GFP + rol-6(su1006)] V. Rollers. Strain is bioluminescent when provided with exogenous D-luciferin (potassium salt) due to sur-5 promoter driving expression of firefly (Photinus pyralis) luciferase (lacking the peroxisome tagging signal) fused in-frame to GFP(S65C). Pick animals with high levels of fluorescence to retain expression of luciferase transgene. This strain is for academic use only. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. References: Lagido C, et al. BMC Physiol. 2008 Apr 2;8:7. McLaggan D, et al. PLoS One. 2012;7(10):e46503. Lagido C, et al. Toxicol Sci. 2009 May;109(1):88-95.
RB754 C. elegans aak-2(ok524) X. Show Description
T01C8.1. Homozygous. Outer Left Sequence: TCATTTGCTGCAACTTCCTG. Outer Right Sequence: ATACGTGGCATTTACGGAGG. Inner Left Sequence: ATGTCGTTGGAAAGATTCGC. Inner Right Sequence: AAGGAGTGCTTAACGAGCCA. Inner primer WT PCR product: 2741. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807