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Strain Species Genotype
VC186 C. elegans smo-1(ok359)/szT1 [lon-2(e678)] I; +/szT1 X. Show Description
K12C11.2. Heterozygotes are WT and segregate WT, arrested szT1 aneuploid progeny, Lon-2 males, and homozygous ok359 hermaphrodites (sterile with one or more vulval blips). Pick WT hermaphrodites and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB2578 C. elegans pqn-41(ok3590) III. Show Description
F53A3.6 Homozygous. Outer Left Sequence: agtacccaaaaaccaagggg. Outer Right Sequence: cgtacctcgggaaattcaaa. Inner Left Sequence: gtgacggggaatttgaaaaa. Inner Right Sequence: taggggtaaaattacgcggg. Inner Primer PCR Length: 1288. Estimated Deletion Size: about 700 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB2579 C. elegans Y53F4B.20(ok3591) II. Show Description
Y53F4B.20 Homozygous. Outer Left Sequence: aatcgatatccagcaaaccg. Outer Right Sequence: cggcaaatcggtaaacagtc. Inner Left Sequence: tccggaaaattgtggttttg. Inner Right Sequence: ggaattgaaaatttccggc. Inner Primer PCR Length: 1252. Estimated Deletion Size: about 600 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB2580 C. elegans wdfy-2(ok3592) II. Show Description
D2013.2 Homozygous. Outer Left Sequence: atgatagatccgttcgcctg. Outer Right Sequence: cgagagtttcctggagatgc. Inner Left Sequence: tcatttcatgccagttgctc. Inner Right Sequence: tgtggaattgcaagtggagt. Inner Primer PCR Length: 1294. Estimated Deletion Size: about 600 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB2581 C. elegans Y56A3A.29(ok3593) III. Show Description
Y56A3A.29 Homozygous. Outer Left Sequence: aatcgtctctggctctctgg. Outer Right Sequence: cggtttgcctgcaaataact. Inner Left Sequence: gagcaaacccctgaattttt. Inner Right Sequence: cgaataatttcccatttttgtga. Inner Primer PCR Length: 1166. Estimated Deletion Size: about 500 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB2582 C. elegans tsp-1(ok3594) III. Show Description
C02F5.8 Homozygous. Outer Left Sequence: ccagcattgcaagactcaaa. Outer Right Sequence: aaggactacgccagcttgaa. Inner Left Sequence: ccttgacgtcattccgatct. Inner Right Sequence: tcaaaagtgttagcattaacgaaa. Inner Primer PCR Length: 1252. Estimated Deletion Size: about 600 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB2583 C. elegans Y65B4BL.2(ok3595) I. Show Description
Y65B4BL.2 Homozygous. Outer Left Sequence: taaccaagattcggagacgg. Outer Right Sequence: ctgaagttcgaacagcacga. Inner Left Sequence: atgtacgtgaattccctccg. Inner Right Sequence: ttcggcctgaaaattgaaag. Inner Primer PCR Length: 1363. Estimated Deletion Size: about 900 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB2584 C. elegans F11A6.2(ok3596) I. Show Description
F11A6.2 Homozygous. Outer Left Sequence: actaacaaatggggcagcac. Outer Right Sequence: gcgagctttgaacttttgct. Inner Left Sequence: caaaaatgttgctcccatca. Inner Right Sequence: ttttctttcagttcagcccc. Inner Primer PCR Length: 1196. Estimated Deletion Size: about 600 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB2585 C. elegans ZK757.2(ok3597) III. Show Description
ZK757.2 Homozygous. Outer Left Sequence: acacacacacacacacacgg. Outer Right Sequence: ccaacgggaccatttatcac. Inner Left Sequence: cacaagcaaatcacttccga. Inner Right Sequence: gggggtggaatggagagtag. Inner Primer PCR Length: 1293. Estimated Deletion Size: about 500 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB2586 C. elegans srg-4(ok3598) III. Show Description
T12A2.12 Homozygous. Outer Left Sequence: tcacaaatccggaggaaatc. Outer Right Sequence: tccaaagcattcgacagttg. Inner Left Sequence: atgctgtggcaacgcaga. Inner Right Sequence: tgcatgctggatttttgttc. Inner Primer PCR Length: 1313. Estimated Deletion Size: about 600 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC2897 C. elegans gpi-1(ok3599)/hIn1 [unc-101(sy241)] I. Show Description
Y87G2A.8. Apparent homozygous lethal deletion chromosome balanced by unc-101-marked inversion. Heterozygotes are WT, and segregate WT, Unc-101 hIn1 homozygotes, and ok3599 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: TGTCTGAGCCTCAACCAAAA. External right primer: CTCTCACTCAAAATGCGGGT. Internal left primer: CAGAATTTTGAGAAAATCCAACG. Internal right primer: AGTTTGTAGCCCCTCAGCCT. Internal WT amplicon: 1205 bp. Deletion size: 621 bp. Deletion left flank: ACCAAATCGGACCGAATGTGCACTTCGTGT. Deletion right flank: ATCAGTTGATTCATCAGGGTACTCGACTGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807