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Strain Species Genotype
VC223 C. elegans tom-1(ok285) I. Show Description
M01A10.2a. Superficially wild type. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB2139 C. elegans F54D12.7(ok2852) II. Show Description
F54D12.7. Homozygous. Outer Left Sequence: TGGCTAAATCCAACTCCGAC. Outer Right Sequence: GGAAAAATGTGCAAGGGGTA. Inner Left Sequence: TGCCAGGTAATTTCCAGTTG. Inner Right Sequence: AAAACTGACGGAGAATTCGAT. Inner Primer PCR Length: 1210 bp. Deletion Size: 250 bp. Deletion left flank: ATTTGTGCTTCCCGATAAGTCCATCCAGTT. Deletion right flank: TTGTTACAGCCATCCGTGAAAGTGTATTCT. Insertion Sequence: TGTTACAGCCATCCGTGAAAG. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB2140 C. elegans mec-9(ok2853) V. Show Description
C50H2.3. Homozygous. Outer Left Sequence: AACTCTCGGAGCACAAATGC. Outer Right Sequence: TTGGCACAACAACGACAAGT. Inner Left Sequence: GAATTGTAAGCAGGGACATCG. Inner Right Sequence: TTGGCGTCATCGTCAAAAT. Inner Primer PCR Length: 1113 bp. Deletion Size: 408 bp. Deletion left flank: TCCACCAATTCCACCACCGGTTTCTTTTAA. Deletion right flank: AGCACCGTTCAAACAGGGTTTTTCGGCGCA. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB2141 C. elegans F53B6.2(ok2854) I. Show Description
F53B6.2. Homozygous. Outer Left Sequence: GCTGTTCACGTGCTTTTTCA. Outer Right Sequence: AATGAGCAGGGTTTTTGTGG. Inner Left Sequence: CATTTGGTGCGGTAAGCAAT. Inner Right Sequence: TTCCAGATCAAGAGCAACCA. Inner Primer PCR Length: 1290 bp. Deletion Size: 962 bp. Deletion left flank: TCTTTGCTCACTTGAACTCGTTGTCTATTC. Deletion right flank: ATCGCATTGAGACCATTGTACAGACTTTCC. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB2142 C. elegans K09C4.1(ok2855) X. Show Description
K09C4.1. Homozygous. Outer Left Sequence: TTAGCCACTTCCTTCCAAGC. Outer Right Sequence: AAGCTGATTGAATGATGCCC. Inner Left Sequence: TTCTTGGAATACAAAGCCCG. Inner Right Sequence: CAACTGACCTGTCCAACTGC. Inner Primer PCR Length: 1253 bp. Deletion Size: 1070 bp. Deletion left flank: GTAAATTTCAATTTATGGAATTTCAACAAT. Deletion right flank: AAACTCAATAAGAGTGTTGTTTGTTACAGA. Insertion Sequence: GTTTGTAA. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB2143 C. elegans W02D9.3(ok2857) I. Show Description
W02D9.3. Homozygous. Outer Left Sequence: CACAATTTTCTCCTCGCGTT. Outer Right Sequence: TGTAGGCCTGATGTGGATGA. Inner Left Sequence: AAGAAGGTCCGACTCCACAA. Inner Right Sequence: GGGCTCAAAAGTGGACAAAA. Inner Primer PCR Length: 1114 bp. Deletion Size: 476 bp. Deletion left flank: AAGCCTGCACGAGAAACGCGCCAACGTCCA. Deletion right flank: AAAGTACGTAAACACCGTACACTCTACGCA. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB2144 C. elegans F26E4.3(ok2858) I. Show Description
F26E4.3. Homozygous. Outer Left Sequence: CCCAGGCTCCTGTAATTTGA. Outer Right Sequence: AGGTTTGAAAATTTGCAGCG. Inner Left Sequence: CCAAAGTTCGTAGAGCCTCG. Inner Right Sequence: CACCTCTAATTTTGAAGAGTTTACA. Inner Primer PCR Length: 1165 bp. Deletion Size: 313 bp. Deletion left flank: TTCGTAAAACAATTGACACGGGAAAACGTT. Deletion right flank: GAGTTGATTCTACCTTCCGATATGATGGCT. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB2145 C. elegans tir-1(ok2859) III. Show Description
F13B10.1. Homozygous. Outer Left Sequence: CGGGAGATAGAAGGCATCTG. Outer Right Sequence: ACGGGGCTTAATTTCCTCAT. Inner Left Sequence: AAACCTTCGAAAATCGAGCA. Inner Right Sequence: CTGCCAGTCCTGTTGCTCTT. Inner Primer PCR Length: 1356 bp. Deletion Size: 354 bp. Deletion left flank: ATTTGTGAGCATTTGGTAGGTGTAAACAGA. Deletion right flank: GGAGGCCACTTCTTTTAATGCTTGGATTGC. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC2236 C. elegans pbs-4(ok2856) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
T20F5.2. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2856 homozygotes (early larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CTGCAAAAAGCTGCGATATG. External right primer: GCAAACAGCTGCTGAAACAA. Internal left primer: ATGCACTTGGCACCTTGATT. Internal right primer: CGGAAGAAAACTGGGAGAAA. Internal WT amplicon: 1272 bp. Deletion size: 675 bp. Deletion left flank: GTCAAATAGTGCGCATTGGGCGCGCACACC. Deletion right flank: ATTATGGATCGTGAATACAAAAAAGGTAAG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC2243 C. elegans tufm-2(ok2850) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
C43E11.4. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2850 homozygotes (sterile adult). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CGGCTATGAAAGTCCTCTGC. External right primer: CGACTCTCCCCGACATTTTA. Internal left primer: TATTCCCACACTGATTGCCC. Internal right primer: TGAGCAGATCAGGTAAAACTGC. Internal WT amplicon: 1303 bp. Deletion size: 499 bp. Deletion left flank: CGGATTTCATCAAAAATATGATTTGCGGAA. Deletion right flank: TGTTTTTCAGGAACAGTCATCGTCGGTACA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC2493 C. elegans +/mT1 II; ccdc-55(ok2851)/mT1 [dpy-10(e128)] III. Show Description
C16C10.6. Apparent homozygous lethal deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes), and ok2851 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: ATCTTTTGCTGCGTCCAAAC. External right primer: ATGCCCGTATTGCAAAGAAC. Internal left primer: TTTTTGTCAATTTTTCGGGA. Internal right primer: CAGAGAATGTTTAAAAATCCGTTAG. Internal WT amplicon: 3016 bp. Deletion size: 1577 bp. Deletion left flank: CTCCCTGATTCTCTCGATTCTATGGACTTC. Deletion right flank: AAGAATAAATAAAGTCGTAATTTTTTTTTC. Insertion Sequence: TCTCGATTCTATGGACTAAAATTAAACAGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807