Temperature-sensitive. Maintain at 15C. Animals form germline tumors that prevent fertility at restrictive temerature (25C). This is the first strain reported to be used for the isolation of germ cells for in vitro culture. This strain allows germ cells to remain viable for longer periods than other tumorous mutant strains tested. Reference: Chaudhari SH, et al. Dev Cell. 2016 Jul 11;38(1):33-46.

T05A6.2 Homozygous. Outer Left Sequence: gataatctggtttgccgcat. Outer Right Sequence: ggtgtgattggtgcagagaa. Inner Left Sequence: ttgaatcgaaacgccttttt. Inner Right Sequence: aaacaagagcgaaggtcgaa. Inner Primer PCR Length: 2262. Deletion size: about 1600 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807