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Strain Species Genotype
ET100 C. elegans H01G02.2(ok200) IV. Show Description
H01G02.2. External left primer: TGCATCCCTTTGATTCCTTC. External right primer: AAACCTGGGCGCTTTTATTT. Internal left primer: GCAATCCTTGCTTGATCCAT. Internal right primer: TGATTGCAACGTTCCATGAT. Internal WT amplicon: 3033 bp. Deletion size: 1251 bp. Deletion left flank: AAACTCACTTTTGAAACATTCGGGACCATT. Deletion right flank: GATGAAGATCATGGAACGTTGCAATCAATT. This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. URL: http://www.celeganskoconsortium.omrf.org.
CB6746 C. elegans mif-1(ok2009) III. Show Description
No obvious phenotype, normal sensitivity to bacterial pathogens. Derived from RB1633; further out-crossed into wild-type background. Reference: O’Rourke et al. (2005) PMID: 16809667.
RB1625 C. elegans par-1(ok2001) V. Show Description
H39E23.1. Homozygous. Outer Left Sequence: ATGTCGGCGGCTGATATTAC. Outer Right Sequence: ACGGAGTCAATTTTTGACGG. Inner Left Sequence: GAGTGAACGAGAAAAAGGCG. Inner Right Sequence: CCACGTCAATCCGAGAGTTT. Inner Primer PCR Length: 2534 bp. Deletion Size: 594 bp. Deletion left flank: ACACTTTTCGCTTCGGGATCAGAGATTCCT. Deletion right flank: TCATTCGATGCTGACTGCTTGCGGGGCCAT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB1626 C. elegans F38B7.1(ok2002) V. Show Description
F38B7.1. Homozygous. Outer Left Sequence: TTCGGACCCATCTTCACTTC. Outer Right Sequence: ATTTCGATTGCTGCGTCTCT. Inner Left Sequence: CTTTTCAAACAAGCGCACAA. Inner Right Sequence: TCCCCAAAACAGCACTAACC. Inner Primer PCR Length: 2805 bp. Deletion Size: 1981 bp. Deletion left flank: AGTTTTTGATTGGGTTTTCACCTCCACTCA. Deletion right flank: AGATTCAATATTTTTTAAGTTCTGTGAAAA. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB1627 C. elegans pll-1(ok2003) III. Show Description
K10F12.3. Homozygous. Outer Left Sequence: AAAACACCAATCAGGTTCGC. Outer Right Sequence: TGACCGGATAGAATTCGGAG. Inner Left Sequence: CACATGGCAAATTTAGGGCT. Inner Right Sequence: TTCAAGCAGACCATCTGCAC. Inner Primer PCR Length: 3277 bp. Deletion Size: 1120 bp. Deletion left flank: CAATCTCATGACAGTCGACGGCTTCACATC. Deletion right flank: TACCAATATATGTAATAAAGGACGATAATT. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB1628 C. elegans sulp-4(ok2004) V. Show Description
K12G11.1. Homozygous. Outer Left Sequence: CGGATAAACCATGCAAGACA. Outer Right Sequence: CATCACGCATTGTTGGGTAG. Inner Left Sequence: CCCCAATTTCTTAAGGCACA. Inner Right Sequence: TACCAGCTTAAAGCGGCAAT. Inner Primer PCR Length: 2943 bp. Deletion Size: 2119 bp. Deletion left flank: AACTTCCAGGCATGCCAGTTTAGGTATGTA. Deletion right flank: AACACTCATTCTCCTGATATTTTATCTCGA. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB1629 C. elegans R07E3.3(ok2005) X. Show Description
R07E3.3 Homozygous. Outer Left Sequence: agccagttgtctcccatttg. Outer Right Sequence: agaaccggacattgttgagc. Inner Left Sequence: tggcacctatacgaccatga. Inner Right Sequence: gggaaaatgtggagggaact. Inner Primer PCR Length: 2636. Deletion size: about 1200 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB1630 C. elegans exoc-7(ok2006) I. Show Description
C43E11.8. Homozygous. Outer Left Sequence: AGAAGGCCATCTTGCTCATC. Outer Right Sequence: TCCAATGGCAAGTGATCAAA. Inner Left Sequence: CAGAGCAGAGAGGATACGGG. Inner Right Sequence: GAACGGGAAATTGCAAAAGA. Inner Primer PCR Length: 3229 bp. Deletion Size: 1803 bp. Deletion left flank: CTCGATCACTTCATTCACATATGAAGAACA. Deletion right flank: ATTAAAAAAGTACTTTTTTTTAATCGAAAA. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB1631 C. elegans ser-3(ok2007) I. Show Description
K02F2.6. Homozygous. Outer Left Sequence: CTAACTGCTCCGCCTCAAGT. Outer Right Sequence: AATTTGGCAGCCATTTTCAG. Inner Left Sequence: TGCGTGAATCACCATCACTT. Inner Right Sequence: TTAAACGGCCAATTTATCGC. Inner Primer PCR Length: 2855 bp. Deletion Size: 1244 bp. Deletion left flank: GTTACCAGATTTGTGTGGAGTGGCAGAATA. Deletion right flank: ATGTGATGGGTTTCTTGACCATTCGTAGAA. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB1632 C. elegans T02E9.1(ok2008) V. Show Description
T02E9.1. Homozygous. Outer Left Sequence: TAAGCTCAACAGCTGCCTCA. Outer Right Sequence: CCAGATGAGACCTCTTTCCG. Inner Left Sequence: GCATCGATTTTGTTCGCTTT. Inner Right Sequence: GGTCAGTGGGATACGGATGT. Inner Primer PCR Length: 3079 bp. Deletion Size: 1209 bp. Deletion left flank: TTCTTTTGTCTTTCATTTTCCCTTCACACG. Deletion right flank: GAAATCTCCAGAAGAAAGTGACGACAAGAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB1633 C. elegans mif-1(ok2009) III. Show Description
Y56A3A.3. Homozygous. Outer Left Sequence: CCATGTCAACAAAGTCCGTG. Outer Right Sequence: GAATGAAAAATCCAAGGCGA. Inner Left Sequence: CGGTAAATTTCCCCGATTTT. Inner Right Sequence: TTCGTTGGATTTTTCCTTCG. Inner Primer PCR Length: 2533 bp. Deletion Size: 1020 bp. Deletion left flank: AGGCCCCACAGAAAGGAGGCCCCACCACGG. Deletion right flank: CTTTAAACCTATGTGCACTACCAGATAAAA. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1530 C. elegans mei-1(ok2000) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
T01G9.5. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2000 homozygotes (sterile, lays eggs that don't hatch). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: TAATTGTTTGTCGCGGATGA. External right primer: GATGAAGGTGGCCTTGAAAA. Internal left primer: TGTTTCCAACAAGTGAGCCA. Internal right primer: CAAAAACCAAAGCTAGGCCA. Internal WT amplicon: 2180 bp. Deletion size: 1378 bp. Deletion left flank: ACAAAGAAAGGAGTTGGAGCAGCAGGTCCA. Deletion right flank: CAAAGAATGGTGTGACTCTTTTGGTGCCAT. Insertion Sequence: TGTAAATCAACTATTTATTGTGATCTCCTTTTAGTTTAAAATATTGTGGCCTAGCTTTG GGTTTTTGAAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807