zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)] IV. Tumorous germline with degeneration of surrounding extracellular matrix & disrupted gonadal basement membrane. Reference: Qi W, et al. PLoS Genet. 2012;8(8):e1002836. doi: 10.1371/journal.pgen.1002836.

No obvious phenotype. The primers used to isolate (ok198)were: LS969.E1: TGAGCTCGGAGATGTTGCT; LS969.E2: CCGGTCATTCCTCATTCACT; LS969.I1: GGGAGGGTCTTACGTTGTGA; LS969.I2: GTCGAAAAATCAACTTGCGG; The deletion band runs at about 2000bp. The wt band (based on the inside primers) is 3195bp making the deletion about 1200bp of the gene F54A5.3.

F09B12.1. Homozygous. Outer Left Sequence: ATTTCCATTCTCGGCTCCTT. Outer Right Sequence: CTCTCACACCGTCATCGAAA. Inner Left Sequence: GGCTGCTTCGATTTCTTCAG. Inner Right Sequence: CGAACCTTTTTGCATGTGTG. Inner Primer PCR Length: 2791 bp. Deletion Size: 1289 bp. Deletion left flank: TTCGTGCTCAAATTTCAAAATAAATGTGCA. Deletion right flank: GTGACATTAAACGTCTCTTGACGTGTTCCG. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F35C11.1. Homozygous. Outer Left Sequence: AGATGCCGACGTCAATTTTC. Outer Right Sequence: ACTGTTGGGCCATACTCGAC. Inner Left Sequence: AATTCGGCTCAAAAAGCTCA. Inner Right Sequence: AGGGACCGAAGAGTGGATTT. Inner Primer PCR Length: 2278 bp. Deletion Size: 1477 bp. Deletion left flank: GCGTTCCAAATTTTATATCAACGGTTGATG. Deletion right flank: AAATTAATTTACCAGTTTATTGTATTGTCT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

ZK1307.8. Homozygous. Outer Left Sequence: ACACAAAGAAATTGGGGCTG. Outer Right Sequence: AAATTCAAAGAGTTCCCGCC. Inner Left Sequence: ATATCGGCATGAGACCCATC. Inner Right Sequence: TCATTCAAGCGAAACAAGGA. Inner Primer PCR Length: 2131 bp. Deletion Size: 1446 bp. Deletion left flank: ACTGACACCTTCCGATGCTTGGACGGATCC. Deletion right flank: TGACAAGGAAGTTGTTCACGAGGAACTTTA. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

lin-17(os160) identified and reported by Hitoshi Sawa (personal communication). Phenotype is Egl. Bivulva. Psa (Phasmid socket absent). F52C9.8. Homozygous. Outer Left Sequence: GAGCACAGCACAGATGAAGC. Outer Right Sequence: ATGGCATTTTCGCAAGAAAC. Inner Left Sequence: CCTTCTAACGCTTTACCCCC. Inner Right Sequence: GTCCAGTGGATCCGAGTTGT. Inner Primer PCR Length: 3247 bp. Deletion Size: 1330 bp. Deletion left flank: ACATACTGGAGCTGCTCTGCTTCTCGAATG. Deletion right flank: TGGCGCCGAATACGATTTTGATTAGCGCGA. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F02C9.3. Homozygous. Outer Left Sequence: GACGGAACACGAGTGGAGAT. Outer Right Sequence: CAAAGCTAGGCGGTGAAAAC. Inner Left Sequence: TGCAGATGTTGTGTTGCTGA. Inner Right Sequence: TGGTGATGAAGACCCATGAA. Inner Primer PCR Length: 3263 bp. Deletion Size: 1195 bp. Deletion left flank: AATGGACAGAAACCGTCGGTGTAAAACTTG. Deletion right flank: CGACAGCGTCCGACAGCGGGCGACAGCGGG. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

K07C6.5. Homozygous. Outer Left Sequence: CTCGGCTTGAATAATGGGAA. Outer Right Sequence: AAACTACGCTTTGGCGAGAA. Inner Left Sequence: CCGCCCTTAAAAAGTTACCC. Inner Right Sequence: AAACAAATCCCACCGACAAA. Inner Primer PCR Length: 2497 bp. Deletion Size: 807 bp. Deletion left flank: ACTTGTGGCTGACTGGTCAAGAAACCACGA. Deletion right flank: TTTTAAACATATAGGTTTTTCGCATTTTAA. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

Y53C12A.2. Homozygous. Outer Left Sequence: TGAGAATTTGGGAACATCGG. Outer Right Sequence: ATCAATCGTTCCATGCATCA. Inner Left Sequence: ATTTGCTCAGAAAACGGGAA. Inner Right Sequence: ATTCAGCCATCATGGGAATC. Inner Primer PCR Length: 2194 bp. Deletion Size: 1158 bp. Deletion left flank: TCTTCTCAGCTTCTGCAACGTCAGCATTCA. Deletion right flank: TAGTTAGTTTGCACTTGAGAAAATGTTGCT. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

C04D8.1. Homozygous. Outer Left Sequence: CTGCCACGTCAAAAGAGGAT. Outer Right Sequence: AGCGTTTCCCTCAACTCTCA. Inner Left Sequence: AGGCCATGATGATCCAGAAG. Inner Right Sequence: GTTGACCAGGAAGATGGGAA. Inner Primer PCR Length: 2938 bp. Deletion Size: 1479 bp. Deletion left flank: CAGTTTGTGTAAGTGTGTATGTGGTCATTT. Deletion right flank: TATTATCCAATACTTTTTAACTACTGTCAT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

Y71H2AM.12. Superficially wild type. External left primer: GCGAAAAAGGTAACTGCTGG. External right primer: GTTGAGGCCCAAAAATCAAA. Internal left primer: ATGCAGCACCTCCTCGTAAC. Internal right primer: GTTGTGGTTGTTGGGGAATC. Internal WT amplicon: 2336 bp. Deletion size: 1365 bp. Deletion left flank: AATTTCCTGTCACGTACTCATTGCACCACA. Deletion right flank: TCGATTATTCCCTTCCATCGGATCAAATGT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807