qIs51 [myo-2p::GFP + pes-10p::GFP + F22B7.9p::GFP]. Heterozygotes are wild-type GFP+ and segregate non-GFP ok197 homozygotes (viable, sterile), wild-type GFP+ heterozygotes, and arrested nT1[qIs51] aneuploids. rad-50 homozygotes are viable, produce more than 95% dead eggs and a large fraction of the survivors are male (Him phenotype). Pick wild-type GFP+ and check for correct segregation of progeny to maintain. Reference: Hayashi M, et al. PLoS Genet. 2007 Nov;3(11):e191.

R107.1 Homozygous. Outer Left Sequence: cacccctgtaggcctgatta. Outer Right Sequence: tacgcctggcttttgtaggt. Inner Left Sequence: tattaaggcatgcggtaggc. Inner Right Sequence: ccaaaaggaaatgaaaagcg. Inner Primer PCR Length: 2870. Deletion size: about 1500 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F10B5.4. Homozygous. Outer Left Sequence: ATGATGATGGGACGGAACAT. Outer Right Sequence: TTTTGACACACCACACGCTT. Inner Left Sequence: TGGGACAAGGAAAATCGAAC. Inner Right Sequence: CGGCTTGTTTCCAATCATTT. Inner Primer PCR Length: 2809 bp. Deletion Size: 1676 bp. Deletion left flank: AATTGCATTGAAACCTTTAAAAAGAGTTTC. Deletion right flank: ATGCTCACAGAAACCGATGAGTTATCAGAA. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

T25G12. Homozygous. Outer Left Sequence: GAGATAGGCAATCCCGAACA. Outer Right Sequence: GTCGACGTGTCATTTTGTGG. Inner Left Sequence: AGTGCGATGTGACGAGTCTG. Inner Right Sequence: CAAGCTAGATGTGATGGCGA. Inner Primer PCR Length: 2152 bp. Deletion Size: 871 bp. Deletion left flank: TTACATTTCCATTCTTTCAGACTAACTAAAGCGTA. Deletion right flank: AAAATTTTTTAAAGGTTTGCAACTCTTATT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

B0198.3. Homozygous. Outer Left Sequence: CGAACAACGCTATAGGAGCC. Outer Right Sequence: TTTAGTGAAACCCCCACGAG. Inner Left Sequence: CAGAAGAAGATGCCGAGTCC. Inner Right Sequence: ACAGCATCCCTTACATTGCC. Inner Primer PCR Length: 3350 bp. Deletion Size: 1810 bp. Deletion left flank: CAATATACCTCAAACACTTCATCACTCAAG. Deletion right flank: GGAATTCAAATATCAATAATGCTCTGAATA. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F54H5.4. Homozygous. Outer Left Sequence: CCGAAAGAGAGTGAAGACGG. Outer Right Sequence: TTTGTTGTTCCTCCAGGTCC. Inner Left Sequence: AAAGCAAAAATGACATCGCC. Inner Right Sequence: GCAAAAGAGGATGGGAATCA. Inner Primer PCR Length: 2642 bp. Deletion Size: 1658 bp. Deletion left flank: TAGAAATCCACCATATTATACGGAAGTGAC. Deletion right flank: TACATCAAGCGAGCGATCGCCACTGCAGCG. klf-3 was formerly known as mua-1. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F08H9.4, F08H9.12. Homozygous. Outer Left Sequence: CGAGACTGCCACATAGGTCA. Outer Right Sequence: TCGTGAGAACTAATTCGGGG. Inner Left Sequence: TCTTGATCGCGAAATAACCC. Inner Right Sequence: GTTGAAGGCAAACCCACATT. Inner Primer PCR Length: 2255 bp. Deletion Size: 1342 bp. Deletion left flank: TATCCAAATGCGAAAGTTGGCAATGTTTAC. Deletion right flank: AAACGTACTCATGTAGATAAATGTGTTGCT. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F08B4.2. Homozygous. Outer Left Sequence: TAGCAGCACGATATTGCCAG. Outer Right Sequence: CAAGAGCAACAGTTACCGCA. Inner Left Sequence: CAAGGCGTTTGGAGTGAAAT. Inner Right Sequence: AGGAATGGGAGATCGAACCT. Inner Primer PCR Length: 3244 bp. Deletion Size: 1695 bp. Deletion left flank: ATTGTATCCGACTGAAATTGCACCTGAAAA. Deletion right flank: CATCTGTGTCTGAAGCAGTCCGTCCTCCAC. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F53A2.6, F53A2.7. Homozygous. Outer Left Sequence: TTCGTGAAGCATATTGCGAG. Outer Right Sequence: GCCCCTTGATAGTGATTCCA. Inner Left Sequence: CCATTTCCTATTTTCCCCGT. Inner Right Sequence: GTATTGTGCGCCCAACTTCT. Inner Primer PCR Length: 2150 bp. Deletion Size: 1047 bp. Deletion left flank: TGCTCCAGTCGCCGAGAAATCCGCCGTCTA. Deletion right flank: TGGGACAGACTGCAGAGAAGTTGGGCGCAC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F23H11.8. Homozygous. Outer Left Sequence: GTCGATTTCCGGTGTGTTTT. Outer Right Sequence: ACGTTGCTGAAATTTTTGGG. Inner Left Sequence: GAAACCGTGCTTTTCAAGGA. Inner Right Sequence: TTTGCCGGAAACTTCAATTC. Inner Primer PCR Length: 2991 bp. Deletion Size: 1552 bp. Deletion left flank: TGCCTACTATTAGCAATTGTGAAGAACCAA. Deletion right flank: AAAACAACGATATGCTACAAAAAATTTAAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F14B4.3. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok1970 homozygotes (early larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GAAAAACAGTTGGCCCAAAA. External right primer: CCATCTCCGTGGAATGTCTC. Internal left primer: ACAAATGGCGATGCATCATA. Internal right primer: GGTGAAGAGCCAATCGAAAA. Internal WT amplicon: 3259 bp. Deletion size: 1241 bp. Deletion left flank: GAAAACTTACCGTGGTGACTGATTATGATC. Deletion right flank: ATTTACGTAGATTTAGGTGCGTCTAAGGGT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807