More Fields
Strain Species Genotype
DG2941 C. elegans spr-5(by134) I; acy-4(ok1806) V. Show Description
spr-5(by134) suppresses acy-4(ok1806) sterility. Reference: Kim S, et al. 2012 Genetics
DG3095 C. elegans twk-1(tn1397) I; acy-4(ok1806) V. Show Description
twk-1(tn1397) suppresses acy-4(ok1806) sterility. acy-4(ok1806) suppresses twk-1(tn1397) adult-onset uncoordinated or paralyzed phenotype. Reference: Kim S, et al. 2012 Genetics
DG3373 C. elegans sacy-1(tn1385) I; acy-4(ok1806) V. Show Description
sacy-1(tn1385) suppresses acy-4(ok1806) sterility. Reference: Kim S, et al. 2012 Genetics
DG3391 C. elegans acy-4(ok1806) V; tnEx37. Show Description
tnEx37 [acy-4(+) + sur-5::GFP]. Pick wild-type to maintain. Segregate GFP+ fertile animals and GFP- acy-4(ok1806) sterile animals. Reference: Kim S, et al. 2012 Genetics
DG3408 C. elegans twk-1(tn1397) I. Show Description
Adult-onset uncoordinated or paralyzed. DG3408 was segregated from DG3095 [twk-1(tn1397) I; acy-4(ok1806) V]. Reference: Kim S, et al. 2012 Genetics
DG3430 C. elegans sacy-1(tn1385) I. Show Description
Superficial wild type. DG3430 was segregated from DG3373 [sacy-1(tn1385) I; acy-4(ok1806) V]. Reference: Kim S, et al. 2012 Genetics
VC1331 C. elegans acy-4(ok1806) V/nT1 [qIs51] (IV;V). Show Description
T01C2.1. Homozygous sterile deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok1806 homozygotes (sterile, lays no eggs). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: TTACATTGATTTGACCGCGA. External right primer: CGCCCGTTTCGATAAAGTAG. Internal left primer: CGACTCGCAACATTCACACT. Internal right primer: AGAGTTGGCATTCATACGGG. Internal WT amplicon: 2936 bp. Deletion size: 1318 bp. Deletion left flank: CTTAAATTCTTCCCGATCCAAAATCTGATC. Deletion right flank: ATAACACTGGTGAGAACGATGAACATTTGT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807