| nlp-79(sy1268) IV. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of nlp-79;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: aaattaaattcaacttttcaggtaaaATGTCCACT
Right flanking sequence: CGGTGGTTTGTGTTCGTCGCCCTGATGGCTCTCG
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : CAGGTAAAATGTCCACTCGG
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616
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