More Fields
Strain Species Genotype
DN20 C. elegans nhr-6(lg6001) III. Show Description
Homozygotes have defective spermatheca development causing low brood size, abnormally shaped eggs, and occasional Egl. nhr-6(lg6000) allele was originally generated by Marius Hoener and Tri Nguyen.
IP1001 C. elegans nhr-6(lg6001)/qC1 [dpy-19(e1259) glp-1(q339)] III. Show Description
Homozygous nhr-6(lg6001) mutants have defective spermatheca development causing low brood size, abnormally shaped eggs, and occasional Egl. Heterozygotes are WT and segregate WT, semi-sterile WT, and Sterile Dpys.
OP90 C. elegans unc-119(ed3) III; wgIs90. Show Description
wgIs90 [nhr-6::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)
JIM193 C. elegans ujIs113 II; ujIs193. Show Description
ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::his-24::mCherry::let-858 3'UTR + unc-119(+)] II. ujIs193 [nhr-67::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence in fosmid ID#WRM0633cC01 by recombineering. Expression of transgene confirmed by GFP.
LA275 C. elegans nhr-67(pf2) IV. Show Description
Pvl. Egl. Weaker allele than pf88 and pf159.
LA691 C. elegans nhr-67(pf159) IV. Show Description
Pvl. Egl. Poor mating.
MU1255 C. elegans nhr-67(tm2217) IV/nT1 [qIs51] (IV;V). Show Description
Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP tm2217 homozygotes (arrested L1 larvae). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain.
MU1268 C. elegans bwIs6. Show Description
bwIs6 [nhr-67::GFP + rol-6(su1006)]. Rollers.
MU1269 C. elegans nhr-67(pf88) IV/nT1 [qIs51] (IV;V). Show Description
Heterozygotes are WT and GFP+. Pick GFP+ to maintain -- viable pf88 homozygotes (Pvl Egl) can overtake the bablanced population. nT1[qIs51] is homozygous lethal. qIs51 is an insertion of ccEx9747 with markers: myo-2::GFP expressed in the pharynx throughout development, pes-10::GFP expressed in the embryo, and a gut promoter F22B7.9::GFP expressed in the intestine. Reference: Verghese E, et al. Dev Biol. 2011 Aug 15;356(2):516-28.
MU1289 C. elegans nhr-67(pf88) IV. Show Description
Pvl. Egl. Poor mating.
OH14711 C. elegans nhr-67(ot795) IV; him-5(e1490) V; otEx5999. Show Description
otEx5999 [nhr-67(fosmid) + unc-47p::mChopti]. Him. ot795 is lethal after L1 stage; all animals L2 and older should carry the extra-chromosomal array. otEx5999 carries fosmid WRM0613bE08; reporter contains 300 bp upstream of the unc-47 start codon. Reference: Gendrel M, et al. Elife. 2016 Oct 14;5.
OH14712 C. elegans nhr-67(ot795) IV; him-5(e1490) V; otEx6001. Show Description
otEx6001 [nhr-67(fosmid) + unc-47p::GFP]. Him. ot795 is lethal after L1 stage; all animals L2 and older should carry the extra-chromosomal array. otEx6001 carries fosmid WRM0613bE08; reporter contains 300 bp upstream of the unc-47 start codon. Reference: Gendrel M, et al. Elife. 2016 Oct 14;5.
OH3926 C. elegans otIs114 I; nhr-67(ot136) IV. Show Description
otIs114 [lim-6p::GFP + rol-6(su1006)]. Shows ASE asymmetry defects. Mildly temperature sensitive. Maintain at 25C.
OH3928 C. elegans otIs114 I; nhr-67(ot202) IV. Show Description
otIs114 [lim-6p::GFP + rol-6(su1006)]. ASE asymmetry defects. Mild temperature sensitivity; maintain at 25 C.
OH4025 C. elegans otIs114 I; nhr-67(ot190) IV. Show Description
otIs114 [lim-6p::GFP + rol-6(su1006)]. Shows ASE asymmetry defects.
OH7566 C. elegans mgIs18 unc-24(e138) nhr-67(ot407) IV; ntIs1 V; otEx3103. Show Description
mgIs18 [ttx-3p::GFP] IV. ntIs1 [gcy-5p::GFP + lin-15(+)] V. otEx3103 [elt-2::GFP]. All worms have mgIs18 and ntIs1. mgIs18 labels AIY interneurons. ntIs1 marks one GFP+ neuron (ASER) in the head. otEx3103 [elt-2::GFP] is expressed in intestinal cells; maintain by picking animals with intestinal GFP. Worms that have lost the array are Emb, Lvl, and have an ASE defect.
OH7631 C. elegans nhr-67(ok631) IV; otEx3362. Show Description
otEx3362 [nhr-67(fosmid)::mCherry + elt-2::GFP]. Pick GFP+ to maintain. otEx3362 rescues nhr-67(ok631).
OH7726 C. elegans otIs114 I; nhr-67(ot158) IV. Show Description
otIs114 contains [lim-6p::GFP + rol-6(su1006)]. Rollers.
OP373 C. elegans unc-119(tm4063) III; wgIs373. Show Description
wgIs373 [nhr-67::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)
OP402 C. elegans unc-119(tm4063) III; wgIs402. Show Description
wgIs402 [nhr-62::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)
OP413 C. elegans unc-119(tm4063) III; wgIs413. Show Description
wgIs413 [nhr-63::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)
PHX509 C. elegans nhr-67(syb509[nhr-67::gfp]) IV. Show Description
GFP reporter inserted into C-terminus of endogenous nhr-67 locus. Reference: Medwig-Kinney TN, et al. Development. 2020 Jan 2;147(1).
RB1015 C. elegans nhr-66(ok940). Show Description
T09A12.4. Homozygous. Outer Left Sequence: GGTATCTGCCTTGTTCTGCC. Outer Right Sequence: GAAACGATGCTCATGCTCAA. Inner Left Sequence: AACCGCGAACAACGAGTTAC. Inner Right Sequence: CAGGGGAACCGCTAGACATA. Inner Primer WT PCR product: 3091. Deletion size: 1317 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB1407 C. elegans nhr-60(ok1600) V. Show Description
F57A10.5. Superficially wild type. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB1425 C. elegans nhr-60(ok1622) V. Show Description
F57A10.5. Homozygous. Outer Left Sequence: ATGTGACGTTCGACTGCAAC. Outer Right Sequence: CCCAACTGAATGCCTGATTT. Inner Left Sequence: CGCCGTTCTAACTGGAATGT. Inner Right Sequence: AAGGAATAGAGCTGTGCCGA. Inner Primer PCR Length: 2872 bp. Deletion Size: 989 bp. Deletion left flank: GGATCAACGGTGCAACAGACATCACAGAAG. Deletion right flank: TATTGTTTTGCTAGTAGCTAATTTAACTGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB1578 C. elegans nhr-69(ok1926) I. Show Description
T23H4.2. Homozygous. Outer Left Sequence: TGCTTTAACGGACGAGTTCA. Outer Right Sequence: ACACCCATCATACGCTCTCC. Inner Left Sequence: AAAGCACAAAAGAAATCCGC. Inner Right Sequence: TCTATGTTTTCCACCCTGGC. Inner Primer PCR Length: 2476 bp. Deletion Size: 1329 bp. Deletion left flank: GTCATTTTTTAAAAAAGAAAGTATGAAACA. Deletion right flank: GAATATATCGCATTTTTTGTTTTATTGGAA. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB1592 C. elegans nhr-64(ok1957) I. Show Description
C45E1.1. Homozygous. Outer Left Sequence: AAATTCGTGTCGAAAATGCC. Outer Right Sequence: GTGGACGGTGTGATCACTTG. Inner Left Sequence: GGGATAGAATTCGACCAGCA. Inner Right Sequence: ATCGTTGCATTTAAGGTGGC. Inner Primer PCR Length: 2771 bp. Deletion Size: 1348 bp. Deletion left flank: AATCACTACCTTATGAAGGTCGAATGAGCA. Deletion right flank: AAATCAATTTCGAACTCAGTTTTCAAATTC. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RW10732 C. elegans unc-119(ed3) III; zuIs178 V; stIs10024; stIs10507. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)]. stIs10507 [nhr-68::H1-wCherry + unc-119(+)].
RW10740 C. elegans unc-119(ed3) III; zuIs178 V; stIs10024; stIs10684. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)]. stIs10684 [nhr-67::H1-wCherry + unc-119(+)].
RW11476 C. elegans unc-119(tm4063) III; stIs11476. Show Description
stIs11476 [nhr-67::H1-wCherry + unc-119(+)].
RW11514 C. elegans unc-119(tm4063) III; stIs11514. Show Description
stIs11514 [nhr-64::H1-wCherry + unc-119(+)].
SD1631 C. elegans ccIs4251 I; stIs10656. Show Description
ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10656 [nhr-69p::HIS-24::mCherry + unc-119(+)]. Reference: Liu, X, et al., Cell (2009).
STE69 C. elegans nhr-66(ok940) IV. Show Description
Reference: Pathare PP, et al. PLoS Genet. 2012 Apr;8(4):e1002645.
STE72 C. elegans nhr-80(tm1011) III; nhr-66(ok940) IV. Show Description
Reference: Pathare PP, et al. PLoS Genet. 2012 Apr;8(4):e1002645.
VC1095 C. elegans nhr-62(gk478) I. Show Description
Y67A6A.2. Superficially wild type. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1527 C. elegans nhr-68(gk708) V. Show Description
H12C20.3. External left primer: CGGTTCTAATCCTCCGTCAA. External right primer: AGCGCACCTGTAAATTGCTT. Internal left primer: TGCCTTGTTTGCCAAGATTT. Internal right primer: CTCCAACCCGTCCTTCTGTA. Internal WT amplicon: 1761 bp. Deletion size: 1301 bp. Deletion left flank: TTATATCATGTTTAGCCCACAAATATTCTA. Deletion right flank: TTTCCGGATGGAACATATTATGATAGAACT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1603 C. elegans nhr-68(gk755) V. Show Description
H12C20.3. External left primer: CGGTTCTAATCCTCCGTCAA. External right primer: AGCGCACCTGTAAATTGCTT. Internal left primer: TGCCTTGTTTGCCAAGATTT. Internal right primer: CTCCAACCCGTCCTTCTGTA. Internal WT amplicon: 1761 bp. Deletion size: 726 bp. Deletion left flank: ACAAAATTTAACCGATTTCAAAAATTATAA. Deletion right flank: TTGAACTCTATCGTATTCTGGGCGGTACTT. Insertion Sequence: T. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC419 C. elegans nhr-67(ok631) IV/nT1 [qIs51] (IV;V). Show Description
C08F8.8. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1 aneuploid progeny, and GFP- ok631 homozygotes (Unc, probable L1 arrest). nT1[qIs51] homozygotes inviable. Pick GFP+ WT and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807