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CGC137
C. elegans
mir-241
(
umn47
[
mir-241
p+
SL1
::egl-13
-NLS
::
mScarlet-I
::c-myc
-NLS
::
linker
::
mODC
(422
-461
)(
E428A
/
E430A
/
E431A
)::
let-858
3'
UTR
]) V.
Show Description
Nuclear mScarlet-I fused to a PEST was inserted in place of the endogenous mir-241 pre-miRNA via CRISPR/CAS9. Left Flanking: CTATTTTTTTCACTTGGATTAGGGG, Right Flanking: GGGATGCTCTTTTTGTACCAAACCG. sgRNA: CCTCAACTTTGACACCCCCG.
SP158
C. elegans
spe-1
(
mn47
)
unc-4
(
e120
)/
mnC1
[
dpy-10
(
e128
)
unc-52
(
e444
)] II.
Show Description
Hets are WT and segregate WT, Sterile Unc-4, and paralysed DpyUnc. Maintain by picking WT.
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