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Strain Species Genotype
MS123 C. elegans med-2(cx9744) III. Show Description
Mos1 insertion into coding region of K04C2.6 (med-2). No obvious phenotype.
VC2546 C. elegans med-2(ok3199) III. Show Description
K04C2.6. External left primer: ATTCCCAAATTCGAAGAGCA. External right primer: CCATCACAACACCACCACAT. Internal left primer: AAATCTCATTATGACAACGAACAAA. Internal right primer: GCATCCAATCCATGCAATTA. Internal WT amplicon: 1203 bp. Deletion size: 580 bp. Deletion left flank: GTGTGGTCTACAGTAAAAGAGCCCAAAGAA. Deletion right flank: GTATCATTGTAAATTGTATAACTCAATTGT. Insertion Sequence: AAGAGCTACAAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RW10197 C. elegans unc-119(ed3) III; zuIs178 V; stIs10024; stIs10137. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)]. stIs10137 [med-2::H1-wCherry + unc-119(+)].
RW10479 C. elegans unc-119(ed3) III; itIs37 IV; stIs10116; stIs10426. Show Description
itIs37 [pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV. stIs10116 [his-72(promoter)::his-24::mCherry::let-858 3'UTR + unc-119(+)]. stIs10426 [med-2::TGF(6.2B3)::GFP::TY1::3xFLAG inserted into fosmid WRM0617dE06 as C-terminal protein fusion].
MLC1480 C. elegans lucIs39. Show Description
lucIs39 [tbx-37p::mNeonGreen::2xNLS::tbx-37 3'UTR + pal-1p::mScarlet-I::2xNLS::tbb-2 3'UTR + med-2p::mScarlet-I::2xNLS::tbb-2 3'UTR]. Wild-type morphology. Integrated array allows for labeling and sorting of ABa and ABp descendants by FACS. Reference: Charest J, et al. Dev Cell. 2020 Sep 24;S1534-5807(20)30672-9. PMID: 33002421