| AG171 |
C. elegans |
mdf-1(av19) unc-42(e270) V. Show Description
Unc. Previously called AG164 by the CGC.
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| DG844 |
C. elegans |
emb-30(tn377) III; unc-46(e177) mdf-1(gk2) V. Show Description
emb-30(tn377ts) conditionally suppresses mdf-1(gk2). Maintain at 15C.
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| KR3784 |
C. elegans |
unc-46(e177) mdf-1(gk2) such-2(h1992) V. Show Description
Unc-46. Slow growing. Lva. Emb. Him. Pick multiple worms to maintain as it is difficult to grow this strain.
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| KR3870 |
C. elegans |
emb-30(h1959) III; unc-46(e177) mdf-1(gk2) V. Show Description
Temperature sensitive allele. Unc-46. Slow growing. Lva. Emb. Him. Pick multiple worms to maintain as it is difficult to grow this strain. Maintain at 20C; 100% Emb at 25C.
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| KR4010 |
C. elegans |
emb-30(h1962) III; unc-46(e177) mdf-1(gk2) V. Show Description
Temperature sensitive allele. Unc-46. Slow growing. Lva. Emb. Him. Pick multiple worms to maintain as it is difficult to grow this strain. Maintain at 20C; sterile at 25C.
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| KR4012 |
C. elegans |
such-1(h1960) III; unc-46(e177) mdf-1(gk2) V. Show Description
Unc-46. Slow growing. Lva. Emb. Him. Pick multiple worms to maintain as it is difficult to grow this strain.
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| KR4063 |
C. elegans |
such-3(h1989) II; unc-46(e177) mdf-1(gk2) V. Show Description
Unc-46. Slow growing. Lva. Emb. Him. Pick multiple worms to maintain as it is difficult to grow this strain.
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| KR4064 |
C. elegans |
such-5(h1987) II; unc-46(e177) mdf-1(gk2) V. Show Description
Unc-46. Slow growing. Lva. Emb. Him. Pick multiple worms to maintain as it is difficult to grow this strain.
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| KR4311 |
C. elegans |
fzy-1(h1988) II; unc-46(e177) mdf-1(gk2) V. Show Description
Temperature sensitive allele. Unc-46. Slow growing. Lva. Emb. Him. Pick multiple worms to maintain as it is difficult to grow this strain. Maintain at 20C; sterile at 25C.
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| DG832 |
C. elegans |
emb-30(tn475)/eT1 III; unc-46(e177) mdf-1(gk2)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-46 Steriles, and Unc-36 (eT1 homozygotes). Maintain by picking WT.
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| KR3627 |
C. elegans |
unc-46(e177) mdf-1(gk2) V/nT1 [let-?(m435)] (IV;V). Show Description
C50F4.11. Strain segregates WT, Uncs (give Sterile progeny or progeny with reduced brood size, all of which arrest before hatching (24%), at various stages of larval development (55%), or later) and dead eggs. Rec'd new stock 11/2002. Attribution: Paper_evidence WBPaper00005604
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| JNC100 |
C. elegans |
unc-119(ed3) III; dotSi100 II. Show Description
dotSi100 [T06E6.2 + unc-119(+)] II. Single copy Mos insertion. Suppresses mdf-1(gk2) sterility. Maintain under normal conditions. References: Tarailo-Graovac M, et al. Cell Cycle. 2010 Dec 15;9(24):4858-65. Tarailo-Graovac M, Chen N. G3 (Bethesda). 2012 Aug;2(8):865-71.
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| JNC144 |
C. elegans |
unc-119(ed3) III; dotSi110 IV. Show Description
dotSi110 [T06E6.2 + unc-119(+)] IV. Single copy Mos insertion. Suppresses mdf-1(gk2) sterility. Maintain under normal conditions. References: Tarailo-Graovac M, Chen N. G3 (Bethesda). 2012 Aug;2(8):865-71.
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| OD2906 |
C. elegans |
mdf-1(lt39[mNG::tev::loxP::3xFlag::mdf-1]) V. Show Description
mNeonGreen and Flag tags inserted at 5' end of endogenous mdf-1 locus using CRISPR/Cas9 engineering. gRNA sequence: tgattgcattaaacatatt Reference: Kim T, et al. Genes Dev. 2017 Jun 1;31(11):1089-1094. doi: 10.1101/gad.302067.117. PMID: 28698300.
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| OD4376 |
C. elegans |
mdf-1(lt167[mScarlet::tev::loxP::3xFlag::mdf-1])V. Show Description
CRISPR/Cas9 engineered. Tagged MDF-1 at its endogenous locus with mScarlet. gRNA sequence: tgattgcattaaacatatt Reference: Lara-Gonzalez P, et al. Science. 2021 Jan 1;371(6524):64-67. doi: 10.1126/science.abc1424. PMID: 33384372.
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| TH238 |
C. elegans |
unc-119(ed3) III; ddIs151. Show Description
ddIs151 [mdf-1::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Cell. 2012 Aug 17;150(4):855-66. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)
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