Temperature sensitive Dpy. Penetrance is incomplete. Left hand roller. Roller low penetrance. Maternal sterile at 25C.

Maternal effect slow growth. See also WBPaper00002456.

Develops slowly (at the clk-3(qm38) rate). Segregates 1/4 Dpy. gro-1(e2400) is maternally rescued. clk-3; gro-1 doubles throw no Dpy and their progeny develop very slowly. To maintain strain pick WT worms singly onto plates and score progeny.

Develops slowly (clk-3(qm38) rate). clk-1(qm30) is maternally rescued. Segregates 1/4 Dpy progeny. To study clk-3; clk-1 doubles place WT worms singly onto plates-the clk doubles will throw no Dpys and their progeny develop extremely slowly (about 3 days to hatch and 10 days for postembryonic development)-they will be sterile when they finally become adults.

Maternally rescued slow growth. Post-embryonic development takes approximately 1.5 days longer then WT at 20C ( clk-2(qm37) is a ts embryonic lethal). Maintain at or below 20C.

Unc. Recessive allele for most phenotypes. Appears to be a gain of function mutation (see Zipkin et al. 1997).

Derived by out-crossing parental strain FX3839 five times.

tmIn20 is an inversion between F46C5.9 and C08H9.13 in LG II.  Inversion strain obtained by Next-generation sequencing.

tmIn21 is an inversion between pck-3 and R09H10.5 in LG IV.  Inversion strain obtained by Next-generation sequencing.

tmIn22 is an inversion between W02G9.9 and 21ur-15544 in LG V.  Inversion strain obtained by Next-generation sequencing.

Superficially wild-type.

nIs281 [myo-2::RFP] integrated near qC1. Recombination between nIs281 and qC1 has been reported. Fails to complemement all markers on qC1. Heterozygotes are WT RFP+ and segregate WT RFP+, Dpy Sterile RFP+ , and tm3846 homozygotes (Emb). Reference: Bowman EA, et al. Development. (In Press).

ckSi6 [cdk-12::GFP + unc-119(+)] I. ckSi6 rescues cdk-12(tm3846). GFP expressed in all nuclei. Reference: Bowman EA, et al. Development. (In Press).

ckSi6 [cdk-12::GFP + unc-119(+)] I. ckSi6 rescues cdk-12(tm3846). ckSi4 [cdk-9::mCherry + unc-119(+)] II. ckSi4 rescues cdk-9(tm2884). GFP and mCherry is expressed in all nuclei. Reference: Bowman EA, et al. Development. (In Press).

qIs26 [lag-2::GFP + rol-6(su1006)]. ckSi26 [cdk-12::GFP::pal-1 3'UTR + unc-119(+)] I. GFP is expressed in all somatic nuclei; expressed in germline only at end of oogenesis. Throws heterozygous Rollers, tm3846 homozygotes (Emb), and tm3846 homozygotes (arrest as L1-L2 larvae). qIs26 was integrated into qC1 and in the process made qC1 homozygous lethal. The distal tip cells are GFP+. qIs26 is an integration of qEx233. Reference: Bowman EA, et al. Development. (In Press).

Large body. Egl.

N-terminal 3xFLAG tag inserted into the endogenous rpa-2 locus using Crispr/Cas9. Generated in N2 background. Reference: Hefel et al., Nucleic Acids Res. 2021 Jan 21;gkaa1293. doi: 10.1093/nar/gkaa1293.

Superficially wild-type. Deletion site verified by PCR. Reference: Meier B, et al. Genome Res. 2014 Oct;24(10):1624-36.

Nicotine-gated current reduced in body wall muscle cells. Disrupted localization of ACR-16 in body wall muscle cells.

Reference: Iwasa H, et al. Aging Cell. 2010 Aug;9(4):490-505.