Search Strains

bsIs20 [tbb-1p::mCherry::tbb-2::tbb-2 3′UTR + unc-119(+)]. Maintain at 20C or lower. dyn-1(luv35) is a CRISPR-engineered mutation reproducing the dyn-1(ky51) allele. GFP inserted at the C terminus of endogenous plk-1 locus. At 26C, embryos exhibit elevated centrosomal PLK-1::sfGFP levels, persistence of PLK-1::sfGFP at centrosomes during late mitosis, and mild defects in intercellular bridge (midbody) assembly. Strain expressing mCherry::tubulin and endogenously tagged PLK-1::sfGFP enables live imaging of spindle dynamics and centrosomal PLK-1 localization during mitosis in embryos. Reference: Dierlam C, et al. Mol Biol Cell. 2026 Apr 1;37(4):br10. doi: 10.1091/mbc.E25-07-0337. PMID: 41705964.

bsIs20 [tbb-1p::mCherry::tbb-2::tbb-2 3′UTR + unc-119(+)]. GFP inserted at the C terminus of endogenous plk-1 locus. Strain expressing mCherry::tubulin and endogenously tagged PLK-1::sfGFP enables live imaging of spindle dynamics and centrosomal PLK-1 localization during mitosis in embryos. Reference: Dierlam C, et al. Mol Biol Cell. 2026 Apr 1;37(4):br10. doi: 10.1091/mbc.E25-07-0337. PMID: 41705964.

GFP inserted at the C terminus of endogenous plk-1 locus followed by LoxP generated by Cre-mediated excision of unc-119(+) cassette. Reference: Martino L. et al. Dev Cell. 2017 Oct. 23; 43(2):157-171.

Small. Male tail abnormal: ray fusions between rays 4 and 5, 6 and 7, 8 and 9. Crumpled spicules. Recessive. Null.