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Strain Species Genotype
TU282 C. elegans lin-32(u282) X. Show Description
Touch insensitive in tail.
OH4226 C. elegans vtIs1 V; lin-32(ot259) X. Show Description
vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Fewer or extra cells expressing dat-1::GFP in various penetrances.
OH4335 C. elegans vtIs1 V; lin-32(ot297) X. Show Description
vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Missing CEPVs and PDEs; ADEs variably affected.
OH6072 C. elegans vtIs1 vsIs33 V; lin-32(ot338) X. Show Description
vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Missing CEPVs and PDEs; ADEs variably affected.
OH7008 C. elegans vtIs1 vsIs33 V; lin-32(ot343) X. Show Description
vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Fewer or extra cells expressing dat-1::GFP in various penetrances.
OH7118 C. elegans vtIs1 vsIs33 V; lin-32(ot366) X. Show Description
vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Missing CEPVs and PDEs; ADEs variably affected.
EM172 C. elegans unc-4(e120) II; him-5(e1490) V; lin-32(bx46) X. Show Description
Missing Rays. Unc.
EM496 C. elegans hlh-2(bx108) I; him-5(e1490) V; lin-32(e1926) X. Show Description
Males are rayless. hlh-2(bx108) strongly and semi-dominantly enhances the ray-loss phenotype of lin-32(e1926).
CB4377 C. elegans lin-22(n372) pal-2(e2260) IV; him-5(e1490) V; lin-32(e1926) X. Show Description
Hermaphrodites segregating males with abnormal ray lineages. Reference: Hodgkin et al. (1989) Genome, 31, 625-637. doi:10.1139/g89-116.
DZ390 C. elegans ezIs10 II; unc-119(ed3) III; him-8(e1489) IV. Show Description
ezIs10 [(pWY3) lin-32::GFP + unc-119(+)].
OP740 C. elegans unc-119(tm4063) III; wgIs740. Show Description
wgIs740 [lin-32::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org).
RW10711 C. elegans unc-119(ed3) III; zuIs178 V; stIs10024; stIs10637. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)]. stIs10637 [lin-32::H1-wCherry + unc-119(+)].
SYS398 C. elegans ujIs113 II; lin-32(dev70([mNeonGreen::lin-32]) X. Show Description
ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::mCherry::his-24::let-858 3Â’UTR + unc-119(+)] II. mNeonGreen knockin at N-terminus of lin-32 locus. Cellular protein expression pattern during embryogenesis (until bean stage) is available at http://dulab.genetics.ac.cn/TF-atlas. Reference: Ma X, Zhao Z, Xiao L, et al. Nat Methods. 2021;18(8):893-902. doi:10.1038/s41592-021-01216-1.
TY2788 C. elegans unc-4(e120) II; yDf19/yDf20 X. Show Description
yDf20 previously known as y288. Internal deletion. Takes out dpy-3 and lin-32. Heterozygotes are Unc.
UL1566 C. elegans unc-119(ed3) III; leEx1566. Show Description
leEx1566 [lin-32::GFP + unc-119(+)]. Superficially wildtype. Grove C. A., De Masi F., et al (2009) Cell 138(2); 314-327.
VL536 C. elegans unc-119(ed3) III; wwIs20; leEx1566. Show Description
wwIs20 [hlh-2::mCherry::his-11 + unc-119(+)]. leEx1566 [lin-32::GFP + unc-119(+)]. Superficially wildtype. Grove C. A., De Masi F., et al (2009) Cell 138(2); 314-327.
SD1648 C. elegans ccIs4251 I; stIs10638. Show Description
ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10638 [lin-32p::HIS-24::mCherry + unc-119(+)]. Reference: Liu, X, et al., Cell (2009).
ST9005 C. elegans ncEx9005. Show Description
ncEx9005 [lin-32p::FLAG::let-363 + lin-32p::Myc::daf-15 + lin-32p::HA::rict-1 + hsp16-2p::plx-1 + rol-6(su1006)]. Rollers. Pick Rollers to maintain. KpnI sites were added by PCR to let-363, daf-15 and rict-1 cDNAs and inserted into pPD49.26 containing lin-32p followed by the FLAG-, Myc- and HA-coding sequences to generate lin-32p::FLAG::let-363, lin-32p::Myc::daf-15 and lin-32p::HA::rict-1, respectively. Reference: Nukazuka A, et al. Nat Commun. 2011 Sep 27;2:484.
ST9013 C. elegans ncEx9013. Show Description
ncEx9013 [lin-32p::FLAG::h4EBP1 + rol-6(su1006)]. Rollers. Pick Rollers to maintain. KpnI sites were added by PCR to h4EBP1 cDNA and inserted into pPD49.26 containing lin-32p followed by the FLAG-coding sequence to generate in-32p::FLAG::h4EBP1. Reference: Nukazuka A, et al. Nat Commun. 2011 Sep 27;2:484.