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Strain Species Genotype
CZ631 C. elegans juIs14 IV. Show Description
juIs14 [acr-2p::GFP + lin-15(+)] IV. GFP expression in cholinergic motor neurons. Reference: Hallam SJ, et al. Development. 2000 Oct;127(19):4239-52.
CZ793 C. elegans vab-1(e2027) II; lin-15B&lin-15A(n765) X; juIs24. Show Description
juIs24 [vab-1::GFP + lin-15(+)]. GFP very faint. Overtly WT.
DA1240 C. elegans adIs1240 lin-15B&lin-15A(n765) X. Show Description
adIs1240 [eat-4::sGFP + lin-15(+)] X.
DA1241 C. elegans eat-4(ky5) III; lin-15B&lin-15A(n765) X; adEx1241. Show Description
adEx1241 [eat-4(+) + lin-15(+)]. Animals with the array are WT. Animals which have lost the array are Muv and Eat. n765 is temperature-sensitive.
DA1242 C. elegans eat-4(ky5) III; lin-15B&lin-15A(n765) X; adEx1242. Show Description
adEx1242 [eat-4(+) + lin-15(+)]. Animals with the array are WT. Animals which have lost the array are Muv and Eat. n765 is temperature sensitive.
DA1243 C. elegans eat-4(ky5) III; adIs1240 lin-15B&lin-15A(n765) X. Show Description
adIs1240 [eat-4::sGFP + lin-15(+)] X.
DA1256 C. elegans lin-15B&lin-15A(n765) X; adEx1256. Show Description
adEx1256 [egl-19::sGFP-NLS + lin-15(+)]. Animals with the array are WT. Animals which have lost the array are Muv. n765 is temperature-sensitive.
DA1262 C. elegans lin-15B&lin-15A(n765) X; adEx1262. Show Description
adEx1262 [gcy-5::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA1266 C. elegans lin-15B&lin-15A(n765) X; adEx1266. Show Description
adEx1266 [gcy-12::GFP + lin-15(+)]. Maintain by picking non-Muv. Very weak GFP signal. n765 is temperature-sensitive.
DA1267 C. elegans lin-15B&lin-15A(n765) X; adEx1267. Show Description
adEx1267 [gcy-8::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA1269 C. elegans lin-15B&lin-15A(n765) X; adEx1269. Show Description
adEx1269 [odr-1::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA1276 C. elegans lin-15B&lin-15A(n765) X; adEx1276. Show Description
adEx1276 [gcy-?::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA1288 C. elegans lin-15B&lin-15A(n765) X; adEx1288. Show Description
adEx1288 [gcy-7::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA1290 C. elegans lin-15B&lin-15A(n765) X; adEx1290. Show Description
adEx1290 [gcy-33::GFP + lin-15(+)]. Maintain by picking non-Muv. gcy-33::GFP in BAG. n765 is temperature-sensitive.
DA1292 C. elegans lin-15B&lin-15A(n765) X; adEx1292. Show Description
adEx1292 [R01E6.1_8k::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA1295 C. elegans lin-15B&lin-15A(n765) X; adEx1295. Show Description
adEx1295 [gcy-32::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA1296 C. elegans lin-15B&lin-15A(n765) X; adEx1296. Show Description
adEx1296 [gcy-32::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA1297 C. elegans lin-15B&lin-15A(n765) X; adEx1297. Show Description
adEx1297 [gcy-6::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA1519 C. elegans lin-15B&lin-15A(n765) X; adEx1519. Show Description
adEx1519 [M03F4.3::GFP + lin-15(+)]. Putative serotonin receptor. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA1616 C. elegans lin-15B&lin-15A(n765) X; adEx1616. Show Description
adEx1616 [ser-4::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA1641 C. elegans lin-15B&lin-15A(n765) X; adEx1641. Show Description
adEx1641 [C09B7.1::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA1644 C. elegans lin-15B&lin-15A(n765) X; adEx1644. Show Description
adEx1644 [K02F2.6::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA1646 C. elegans lin-15B&lin-15A(n765) X; adEx1646. Show Description
adEx1646 [T02E9.3::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA1647 C. elegans lin-15B&lin-15A(n765) X; adEx1647. Show Description
adEx1647 [dop-1(trans)::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA1648 C. elegans lin-15B&lin-15A(n765) X; adEx1648. Show Description
adEx1648 [F01E11.5::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA1649 C. elegans lin-15B&lin-15A(n765) X; adEx1649. Show Description
adEx1649 [F14D12.6::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
DA2356 C. elegans ced-1(e1735) I; ced-3(n717) IV; lin-15B&lin-15A(n765) X; adEx2342. Show Description
adEx2342 [efl-3::mCherry::FLAG + LIN-15(+)]. Maintain by picking non-Muv. Fluorescence is dim, but array is stable (>95% transmission).
DA735 Escherichia coli E. coli. Show Description
Bacteria. 15kb lin-15 Xba fragment in Bluescript. Rescues @ 25 ng/ml and 50 ng/ml, better at 25. Biosafety Level: BSL-1.
DL308 C. elegans ced-3(n717) IV; mxIs28 X. Show Description
mxIs28 [ceh-20p::ceh-20::YFP + lin-15(+)] X. Reference: Potts MB, Wang DP, & Cameron S. Dev Biol. 2009 May 15;329(2):374-85.
DL315 C. elegans lin-15B&lin-15A(n765) X; mxIs30. Show Description
mxIs30 [unc-62p::unc-62::CFP + lin-15(+)]. Reference: Potts MB, Wang DP, Cameron S. Dev Biol. 2009 May 15;329(2):374-85.
DLW14 C. elegans unc-5(lib1[myo-3p::GFP(-) + unc-119(+) + myo-2p::GFP(Mos1)]) IV; krIs14 V. Show Description
krIs14 [hsp-16.48p::MosTransposase + lin-15(+) + unc-122p::GFP] V. Recessive Unc. unc-5(lib1) is a CRISPR/Cas9 engineered mutant carrying the Intersister/Intrachromatid Repair Assay (ICR Assay) cassette inserted into the endogenous unc-5 locus. Briefly, ICR assay cassette includes two tandem GFP cassettes: the upstream using the myo-3 (body wall) promoter with a truncated GFP coding sequence, and the down-stream using the myo-2 (pharynx) promoter with GFP coding sequence interrupted by a Mos1 Drosophila transposon. Excision of Mos1 yields a single DSB, which if repaired by intersister or intrachromatid recombination, then will yield GFP+ progeny. The krIs14 insertion carrying heat-shock inducible Mos1 transposase is marked with coelomocyte GFP expression. Reference: Toraason E, et al. Current Biology 2021. https://doi.org/10.1016/j.cub.2021.03.008
EG1285 C. elegans lin-15B&lin-15A(n765) oxIs12 X. Show Description
oxIs12 [unc-47p::GFP + lin-15(+)]. Integration maps at +2.0 on X. Expression of GFP in all GABAergic neurons.
EG1642 C. elegans lin-15B&lin-15A(n765) X; oxEx166. Show Description
oxEx166 [HSP::MosTRANSPOSASE + CC::GFP + lin-15(+)]. Should be grown at 25C. Maintain by picking non-Muv. Animals carrying the array should be GFP+.
EG1653 C. elegans oxIs22 II. Show Description
oxIs22 [unc-49p::unc-49::GFP + lin-15(+)]. Psoralen integration of oxEx129 [unc-49Bp(long)::GFP + lin-15(+)]. Dorsal and ventral.
EG2288 C. elegans lin-15B&lin-15A(n765) X; oxEx110. Show Description
oxEx110 [unc-49c::GFP + lin-15(+)]. Maintain by picking non-Muv.
EG2762 C. elegans oxEx166. Show Description
oxEx166 [HSP::MosTransposase + coelomocyte::GFP + lin-15(+)]. Should be grown at 25C.
EG4813 C. elegans lin-15B&lin-15A(n765) X; oxEx1088. Show Description
oxEx1088 [unc-17p::halorhodopsin::GFP + Litmus + lin-15(+)]. Maintain by picking wild-type.
EG4814 C. elegans lin-15B&lin-15A(n765) X; oxEx1089. Show Description
oxEx1089 [unc-47p::halorhodopsin::GFP + Litmus + lin-15(+)]. Maintain by picking wild-type.
EG5025 C. elegans oxIs351 X. Show Description
oxIs351 contains [unc-47p::channelrhodopsin::mCherry + lin-15(+) + Litmus]. Superficially wild-type.
EG5027 C. elegans oxIs353 V. Show Description
oxIs353 contains [myo-3p::channelrhodopsin::mCherry + lin-15(+) + Litmus]. Superficially wild-type.
EG5096 C. elegans oxIs364 X. Show Description
oxIs364 contains [unc-17p::channelrhodopsin::mCherry + lin-15(+) + Litmus]. Superficially wild-type.
EG7340 C. elegans oxIs609 X. Show Description
oxIs609 [acr-5p::GAP-43::GFP + lin-15(+)] X. GFP localized to axonal membranes of acr-5 expressing neurons. acr-5 promoter drives expression of reporter containing 40 aa myristoylation sequence of GAP43 fused to GFP. oxIs609 was generated by X-ray integration of oxEx81in N2 background. Reference: Rawson RL, et al. Curr Biol. 2014 Mar 31;24(7):760-5. doi: 10.1016/j.cub.2014.02.025. PMID: 24631238.
EM599 C. elegans him-5(e1490) V; lin-15B&lin-15A(n765) X; bxIs13. Show Description
bxIs13 [egl-5::GFP + lin-15(+)]. Him. egl-5::GFP reporter made from EM#286 (GFP inserted within the last few amino acids of the C-terminus) by integrating bxEx30 in EM588. High nuclear expression of transgene in males (good in seam cells, none in rectal epithelium), weak expression in hermaphrodites. Expression in 2-4 head neurons in males and hermaphrodites. egl-5 gene on reporter does not rescue egl-5(-).
FK430 C. elegans lin-15B&lin-15A(n309) X; ksEx30. Show Description
ksEx30 [dyf-3::GFP + lin-15(+)].
GA631 C. elegans lin-15B&lin-15A(n765) X; wuIs177. Show Description
wuIs177 [ftn-1p::GFP + lin-15(+)]. GFP expression in the intestine. ftn-1p::GFP transgene shows moderate basal expression under standard culture conditions in an otherwise wild-type background, but is strongly induced by reduced insulin/IGF-1 signalling, reduced HIF signalling, and increased free iron levels. References: Ackerman D & Gems D. PLoS Genet. 2012;8(3):e1002498. Valentini S, et al. Mech Ageing Dev. 2012 May;133(5):282-90.
GL347 C. elegans zcIs13 V. Show Description
zcIs13 [hsp-6p::GFP + lin-15(+)] V. Stable transgenic line with GFP expression mainly in the posterior intestine, observed from L1 to adult. Perturbation of mitochondrial folding environment induces robust GFP expression throughout the intestines. Derived by outcrossing parental strain SJ4100 to N2 six times to remove suspected background mutations causing uneven transgene expression and low penetrance of sterility.
GV796 C. elegans ets-4(ok165) lin-15B&lin-15A(n765) X; uzEx710. Show Description
uzEx710 [gly-19p::YFP::ets-4 + lin-15(+)]. Maintain by picking wild-type (non-Muv). Reference: Thyagarajan et al. PLoS Genet. 2010 Sep 16;6(9). pii: e1001125.
GV807 C. elegans ets-4(ok165) lin-15B&lin-15A(n765) X; uzEx721. Show Description
uzEx721 [rab-3p::YFP::ets-4 + lin-15(+)]. Maintain by picking wild-type (non-Muv). Reference: Thyagarajan et al. PLoS Genet. 2010 Sep 16;6(9). pii: e1001125.
GV812 C. elegans ets-4(ok165) lin-15B&lin-15A(n765) X; uzEx726. Show Description
uzEx726 [lin-15(+)]. Maintain by picking wild-type (non-Muv). Reference: Thyagarajan et al. PLoS Genet. 2010 Sep 16;6(9). pii: e1001125.
HA3 C. elegans nuIs11. Show Description
nuIs11 [osm-10::GFP + lin-15(+)]. Array contains pool28; KP#57-59 (osm-10::GFP insert at Nrul site) and pJM24 [lin-15(+) rescues n765 at 20C]. GFP expressed in ASH, ASI, PHA and PHB after 3-fold. nuIs11 may be inserted on LG I.