Search Strains

Homozygous viable. Deletion of 2700 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Left flanking sequence: CAGATTATTTTCATATAGTTGATTCCTGAC ; Right flanking sequence: GCTGGATATAAGCATCGCAGAGACACTCAG. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.

wzIs20 [lgc-55p::GFP + lin-15(+)]. lgc-55 regulatory sequences drive expression of GFP in neurons, GLR cells and some muscles. Reference: Ringstad N, et al. Science. 2009;325(5936):96-100. doi:10.1126/science.1169243

Break points: In(glb-19 lgc-52 In(mec-3 unc-31)) IV. Covered region (Mb) 4.8 (10.5..15.2) Unc Mec. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241.

Break points: In(glb-19 lgc-52 In(mec-3 unc-31)) IV. Covered region (Mb) 4.8 (10.5..15.2) Balancer marked with myo-2p::Venus. Unc Mec. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241.

njIs129 [lgc-55p::flp + flp-22p::frt::tomm-20(N-55AA)::miniSOG + ges-1p::NLS::GFP] I. Targeted ablation of SMDD/V neuron. Reference: Ikeda M, et al. Proc Natl Acad Sci USA. 2020 Mar 2;117(11):6178–6188. PMID: 32123108.

njEx1557 [lgc-55p::flp + flp-22p::frt::HisCl1::SL2::mCherry + ges-1p::NLS::GFP]. Pick fluorescent animals to maintain. Cell silencing of SMDD/V neuron. Reference: Ikeda M, et al. Proc Natl Acad Sci USA. 2020 Mar 2;117(11):6178–6188. PMID: 32123108.

1.463 kb deletion in T21F2.1 encoding ligand-gated chloride channel. Homozygous viable.

1.986 kb deletion in Y113g7A.5 encoding ligand-gated chloride channel. Homozygous viable.

nIs471 [lgc-55::GFP + lin-15(+)]. GFP expression in GLR glia-like cells and head muscles. Reference: Ringstad N, et al. Science. 2009 Jul 3;325(5936):96-100.

flvIs2 [tph-1p(short)::Chrimson + elt-2p::mCherry]. Deficits in serotonin-dependent slowing response. NSM neuron is expressing Chrimson and can be specifically activated by red light. Reference: Dag U, et al. bioRxiv 2023.01.15.524132; doi: https://doi.org/10.1101/2023.01.15.524132.

Endogenous lgc-50 locus tagged with mNeonGreen. Inclusion of the T2A self-cleaving peptide allows mNeonGreen406 to be expressed as a cytosolic protein. Generated in N2 background. Reference: Dag U, et al. bioRxiv 2023.01.15.524132; doi: https://doi.org/10.1101/2023.01.15.524132.

flvIs2 [tph-1p(short)::Chrimson + elt-2p::mCherry]. Deficits in serotonin-dependent slowing response. NSM neuron is expressing Chrimson and can be specifically activated by red light. Reference: Dag U, et al. bioRxiv 2023.01.15.524132; doi: https://doi.org/10.1101/2023.01.15.524132.

flvIs2 [tph-1p(short)::Chrimson + elt-2p::mCherry]. Deficits in serotonin-dependent slowing response. NSM neuron is expressing Chrimson and can be specifically activated by red light. Reference: Dag U, et al. bioRxiv 2023.01.15.524132; doi: https://doi.org/10.1101/2023.01.15.524132.

flvIs2 [tph-1p(short)::Chrimson + elt-2p::mCherry]. Deficits in serotonin-dependent slowing response. NSM neuron is expressing Chrimson and can be specifically activated by red light. Reference: Dag U, et al. bioRxiv 2023.01.15.524132; doi: https://doi.org/10.1101/2023.01.15.524132.

flvIs2 [tph-1p(short)::Chrimson + elt-2p::mCherry]. Deficits in serotonin-dependent slowing response. NSM neuron is expressing Chrimson and can be specifically activated by red light. Reference: Dag U, et al. bioRxiv 2023.01.15.524132; doi: https://doi.org/10.1101/2023.01.15.524132.

flvIs2 [tph-1p(short)::Chrimson + elt-2p::mCherry]. Deficits in serotonin-dependent slowing response. NSM neuron is expressing Chrimson and can be specifically activated by red light. Reference: Dag U, et al. bioRxiv 2023.01.15.524132; doi: https://doi.org/10.1101/2023.01.15.524132.

flvIs2 [tph-1p(short)::Chrimson + elt-2p::mCherry]. Deficits in serotonin-dependent slowing response. NSM neuron is expressing Chrimson and can be specifically activated by red light. Reference: Dag U, et al. bioRxiv 2023.01.15.524132; doi: https://doi.org/10.1101/2023.01.15.524132.

hpIs331 [lgc-55Bp::tomm20::miniSOG::SL2::wCherry + lin-15(+)] X. hpIs321 [nmr-1p::tomm20::miniSOG::SL2::wCherry + lin-15(+)]. ljIs131 [myo-3p::GCaMP3::SL2::tagRFP-T]. Maintain in the covered box to avoid unnecessary exposure to ambient light. Stimulation with blue light (460 nm LED light for 30 min at 4 Hz with 2 mW/mm2) induces mitochondrial-miniSOG ablation of AVA, AVB, AVE, AVD, and RIM premotor interneurons. During neuron ablation, it is recommended to keep the lid of the plate open and use a heat dissipator to keep the air cool. Generated in N2 background. Reference: Gao S, et al. eLife 2018 Jan 23;7:e29915. doi: 10.7554/eLife.29915. PMID: 29360035. Lu Y, et al. Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 10.1016/j.cub.2022.09.002. PMID: 36182701.

hpIs331 [lgc-55Bp::tomm20::miniSOG::SL2::RFP + lin-15(+)]. Maintain in the covered box to avoid unnecessary exposure to ambient light. Stimulation with blue light (460 nm LED light for 30 min at 4 Hz with 2 mW/mm2) induces mitochondrial-miniSOG ablation of AVB. During neuron ablation, it is recommended to keep the lid of the plate open and use a heat dissipator to keep the air cool. Reference: Gao S, et al. eLife 2018 Jan 23;7:e29915. PMID: 29360035

hpIs331 [lgc-55Bp::tomm20::miniSOG::SL2::wCherry + lin-15(+)] X. hpIs321 [nmr-1p::tomm20::miniSOG::SL2::wCherry + lin-15(+)]. ljIs131 [myo-3p::GCaMP3::SL2::tagRFP-T]. Maintain in the covered box to avoid unnecessary exposure to ambient light. Stimulation with blue light (460 nm LED light for 30 min at 4 Hz with 2 mW/mm2) induces mitochondrial-miniSOG ablation of AVA, AVB, AVE, AVD, and RIM premotor interneurons. During neuron ablation, it is recommended to keep the lid of the plate open and use a heat dissipator to keep the air cool. Generated in N2 background. Reference: Gao S, et al. eLife 2018 Jan 23;7:e29915. doi: 10.7554/eLife.29915. PMID: 29360035. Lu Y, et al. Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 10.1016/j.cub.2022.09.002. PMID: 36182701.

hpIs166 [glr-1p::chop-2(H134R)::YFP + lin-15(+)]. YFP expression in glr-1 interneurons. hpEx3239 [lgc-55p::nca-1::GFP + nmr-1p::ATG::nca-1 + nca-1::GFP + myo-2p::RFP]. Pick RFP+ to maintain. Reference: Gao S, et al. 2015 Nature Communications 6, Article number: 6323.

hpIs331 [lgc-55Bp::tomm20::miniSOG::SL2::wCherry + lin-15(+)] X. hpIs321 [nmr-1p::tomm20::miniSOG::SL2::wCherry + lin-15(+)]. hpIs372 [acr-5p::tomm-20::miniSOG::SL2::wCherry + lin-15(+)]. Maintain in the covered box to avoid unnecessary exposure to ambient light. Stimulation with blue light (460 nm LED light for 30 min at 4 Hz with 2 mW/mm2) induces mitochondrial-miniSOG ablation of AVA, AVB, AVE, AVD, RIM premotor interneurons, and B-class motor neurons. During neuron ablation, it is recommended to keep the lid of the plate open and use a heat dissipator to keep the air cool. Generated in N2 background. Reference: Gao S, et al. eLife 2018 Jan 23;7:e29915. doi: 10.7554/eLife.29915. PMID: 29360035.

hpIs371 [unc-4p::tomm-20::miniSOG::SL2::wCherry + lin-15(+)] IV. hpIs331 [lgc-55Bp::tomm20::miniSOG::SL2::wCherry + lin-15(+)] X. hpIs321 [nmr-1p::tomm20::miniSOG::SL2::wCherry + lin-15(+)]. Maintain in the covered box to avoid unnecessary exposure to ambient light. Stimulation with blue light (460 nm LED light for 30 min at 4 Hz with 2 mW/mm2) induces mitochondrial-miniSOG ablation of AVA, AVB, AVE, AVD, RIM premotor interneurons, and A-class motor neurons. During neuron ablation, it is recommended to keep the lid of the plate open and use a heat dissipator to keep the air cool. Generated in N2 background. Reference: Gao S, et al. eLife 2018 Jan 23;7:e29915. doi: 10.7554/eLife.29915. PMID: 29360035.

hpIs331 [lgc-55Bp::tomm20::miniSOG::SL2::wCherry + lin-15(+)] X. hpIs321 [nmr-1p::tomm20::miniSOG::SL2::wCherry + lin-15(+)]. hpIs376 [unc-25p::tomm20::miniSOG::SL2::wCherry + lin-15(+)]. Maintain in the covered box to avoid unnecessary exposure to ambient light. Stimulation with blue light (460 nm LED light for 30 min at 4 Hz with 2 mW/mm2) induces mitochondrial-miniSOG ablation of AVA, AVB, AVE, AVD, RIM premotor interneurons, and D-class motor neurons. During neuron ablation, it is recommended to keep the lid of the plate open and use a heat dissipator to keep the air cool. Generated in N2 background. Reference: Gao S, et al. eLife 2018 Jan 23;7:e29915. doi: 10.7554/eLife.29915. PMID: 29360035.

hpIs331 [lgc-55Bp::tomm20::miniSOG::SL2::wCherry + lin-15(+)] X. hpIs321 [nmr-1p::tomm20::miniSOG::SL2::wCherry + lin-15(+)]. Maintain in the covered box to avoid unnecessary exposure to ambient light. Stimulation with blue light (460 nm LED light for 30 min at 4 Hz with 2 mW/mm2) induces mitochondrial-miniSOG ablation of AVA, AVB, AVE, AVD, and RIM premotor interneurons. During neuron ablation, it is recommended to keep the lid of the plate open and use a heat dissipator to keep the air cool. Generated in N2 background. Reference: Gao S, et al. eLife 2018 Jan 23;7:e29915. doi: 10.7554/eLife.29915. PMID: 29360035.

hpIs603 [lgc-55Bp::tomm20::miniSOG::SL2::BFP + nmr-1p::tomm20::miniSOG::SL2::BFP + acr-5p::tomm20-miniSOG-SL2::BFP + lin-15(+)]. MiniSOG neuron ablation of all premotor interneurons, B-class motor neurons (B-MN), and other neurons. Reference: Gao S, et al. eLife 2018 Jan 23;7:e29915. PMID: 29360035