lgc-27(sy1488) II. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of lgc-27.
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
left flanking sequence: CACACTTTCAGTTGCCGCTTATGATATCGATTGC
right flanking sequence: AAATGGAAAAGCAATATTACAGgttggtgctcaaac
inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: CTTATGATATCGATTGCAAA
Method Reference: G3 (Bethesda).
|