| SP260 |
C. elegans |
mnDp1 (X;V)/+ V; unc-3(e151) let-38(mn141) X. Show Description
STERILE. Maintain by picking WT.
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| KR362 |
C. elegans |
dpy-5(e61) let-388(h88) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h88 arrests as an early larva). Maintain by picking Unc-13 and checking for correct segregation of progeny.
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| KR363 |
C. elegans |
dpy-5(e61) let-387(h87) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h87 arrests as a mid-late larva). Maintain by picking Unc-13 and checking for correct segregation of progeny.
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| KR424 |
C. elegans |
dpy-5(e61) let-383(h115) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the Dup are Unc. Animals which have lost the Dup arrest as early-mid larvae.
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| KR429 |
C. elegans |
dpy-5(e61) let-381(h107) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h107 arrests as an embryo or early larva). Maintain by picking Unc-13 and checking for correct segregation of progeny.
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| OP119 |
C. elegans |
unc-119(ed3) III; wgIs119. Show Description
wgIs119 [let-381::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org).
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| OS13288 |
C. elegans |
let-381(ns995[let-381::gfp::degron]) I. Show Description
Linker with GFP tag and degron inserted at the C terminus of the endogenous let-381 locus. GFP expression in GLR glia, HMC and coelomocytes. Reference: Stefanakis N, et al. 2024 Feb 15. doi: 10.1038/s44318-024-00049-w. PMID: 38360995.
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| SYS668 |
C. elegans |
let-381(dev205([mNeonGreen::let-381]) I; ujIs113 II. Show Description
ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::mCherry::his-24::let-858 3Â’UTR + unc-119(+)] II. mNeonGreen knockin at N-terminus of let-381 locus. Cellular protein expression pattern during embryogenesis (until bean stage) is available at http://dulab.genetics.ac.cn/TF-atlas. Reference: Ma X, Zhao Z, Xiao L, et al. Nat Methods. 2021;18(8):893-902. doi:10.1038/s41592-021-01216-1.
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| VC706 |
C. elegans |
let-381(gk302) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
F26B1.7. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP gk302 homozygotes (sterile DpyUnc). Homozygous hT2[bli-4 let-? qIs48] inviable. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Pick WT GFP and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
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