Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h44 arrests as a late larva or sterile adult). Maintain by picking Unc-13 and checking for correct segregation of progeny.

Animals with the duplication are Unc. Animals which have lost the duplications are DpyUnc and arrest at mid-larval development. Maintain by picking Unc non-Dpy.

Animals with the duplication are Unc. Animals which have lost the duplications are DpyUnc and arrest at mid-larval development. Maintain by picking Unc non-Dpy.

Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h45 arrests as a late larva or sterile adult). Maintain by picking Unc-13 and checking for correct segregation of progeny.

Animals with the duplication are Unc. Animals which have lost the duplications are DpyUnc and arrest at mid-larval development. Maintain by picking Unc non-Dpy. Previously called let-354(h79).

Animals with the Dup are Unc. Animals which have lost the Dup are DpyUnc and arrest in late larval development. See also WBPaper00005456.

Animals with the Dup are Unc. Animals which have lost the Dup are DpyUnc and arrest in late larval development.

Animals with the Dup are Unc. Animals which have lost the Dup are DpyUnc and arrest in late larval development.

Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h94 arrests as a sterile adult). Maintain by picking Unc-13 and checking for correct segregation of progeny.

Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h91 arrests as a late larva or sterile adult). Maintain by picking Unc-13 and checking for correct segregation of progeny.

Strain throws Unc and DpyUncLets. DpyUncLets are sterile adults. Maintain by picking Unc.

Animals with the duplication are Unc. Animals which have lost the duplications are DpyUnc and arrest at mid-larval development. Maintain by picking Unc non-Dpy.

Animals with the duplication are Unc. Animals which have lost the duplications are DpyUncLet (Sterile adults). Maintain by picking Unc non-Dpy.

Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h88 arrests as an early larva). Maintain by picking Unc-13 and checking for correct segregation of progeny.

Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h87 arrests as a mid-late larva). Maintain by picking Unc-13 and checking for correct segregation of progeny.

Animals with the Dup are Unc. Animals which have lost the Dup arrest as early-mid larvae.

Animals with the Dup are Unc. Animals which have lost the Dup are DpyUnc and arrest in mid-late larval development.

Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h107 arrests as an embryo or early larva). Maintain by picking Unc-13 and checking for correct segregation of progeny.

Animals with the Dup are Unc. Animals which have lost the Dup are DpyUncLet and arrest in early larval development. Maintain by picking Unc.

Animals with the Dup are Unc. Animals which have lost the Dup are DpyUnc and arrest in early larval development.

Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h119 arrests as a sterile adult). Maintain by picking Unc-13 and checking for correct segregation of progeny.

Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h126 arrests as a sterile adult). Maintain by picking Unc-13 and checking for correct segregation of progeny.

Animals with the Dup are Unc. Animals which have lost the Dup are DpyUnc and arrest in early larval development.

Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h225 arrests as an early larva). Maintain by picking Unc-13 and checking for correct segregation of progeny.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in early larval development.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in mid larval development.

Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h251 arrests as a mid-stage larva). Maintain by picking Unc-13 and checking for correct segregation of progeny.

h257 arrests in early larval development.

Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h259 arrests as a leaky sterile adult). Maintain by picking Unc-13 and checking for correct segregation of progeny.

Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h262 arrests as an early to mid-stage larva). Maintain by picking Unc-13 and checking for correct segregation of progeny.

Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h271 arrests as a sterile adult). Maintain by picking Unc-13 and checking for correct segregation of progeny.

Animals with the Dup are Unc. Animals which have lost the Dup are DpyUncLet and arrest in late larval (L4) development. DpyUncs may give some progeny that fail to grow. Maintain by picking Unc.

Animals with the duplication are Unc. Animals which have lost the duplication are Dpy Unc and arrest in early mid development.

wgIs119 [let-381::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org).

Linker with GFP tag and degron inserted at the C terminus of the endogenous let-381 locus. GFP expression in GLR glia, HMC and coelomocytes. Reference: Stefanakis N, et al. 2024 Feb 15. doi: 10.1038/s44318-024-00049-w. PMID: 38360995.

Maintain strain by picking WT hermaphrodites. Segregates WT, dead eggs, paralysed DpyUnc and males.

Hets are WT and segregate WT, paralysed DpyUnc, L1 Lethal Unc-4s and males. Maintain by picking WT.

Hets are WT and segregate WT, dead eggs, paralysed DpyUnc and males. Maintain by picking WT.

NOT MALE-RESCUED MATERNAL EFFECT STERILE Maintain by picking WT.

LETHAL EARLY LARVAL. Maintain by picking WT.

Maintain by picking WT.

LETHAL EARLY LARVAL. Maintain by picking WT.

GROWTH SLOW. MALE-RESCUED. STERILE. Maintain by picking WT.

STERILE. Maintain by picking WT.

LETHAL EARLY LARVAL. Pick WT to maintain.

ncEx9005 [lin-32p::FLAG::let-363 + lin-32p::Myc::daf-15 + lin-32p::HA::rict-1 + hsp16-2p::plx-1 + rol-6(su1006)]. Rollers. Pick Rollers to maintain. KpnI sites were added by PCR to let-363, daf-15 and rict-1 cDNAs and inserted into pPD49.26 containing lin-32p followed by the FLAG-, Myc- and HA-coding sequences to generate lin-32p::FLAG::let-363, lin-32p::Myc::daf-15 and lin-32p::HA::rict-1, respectively. Reference: Nukazuka A, et al. Nat Commun. 2011 Sep 27;2:484.

ncEx9007 [unc-54p::FLAG::let-363 + unc-54p::Myc::daf-15 + unc-54p::HA::rict-1 + hsp16-2p::plx-1 + rol-6(su1006)]. Rollers. Pick Rollers to maintain. KpnI sites were added by PCR to let-363, daf-15 and rict-1 cDNAs and inserted into pPD49.26 containing inserted into pPD30.38 containing unc-54p followed by the FLAG-, Myc- and HA-coding sequences to generate unc-54p::FLAG::let-363, unc-54p::Myc::daf-15 and unc-54p::HA::rict-1, respectively. Reference: Nukazuka A, et al. Nat Commun. 2011 Sep 27;2:484.

ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::mCherry::his-24::let-858 3’UTR + unc-119(+)] II. mNeonGreen knockin at N-terminus of let-381 locus. Cellular protein expression pattern during embryogenesis (until bean stage) is available at http://dulab.genetics.ac.cn/TF-atlas. Reference: Ma X, Zhao Z, Xiao L, et al. Nat Methods. 2021;18(8):893-902. doi:10.1038/s41592-021-01216-1.

Temperature sensitive missense allele. Larval lethal and Vul at 25C. About WT at 20C. Previously called let-341.