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Strain Species Genotype
KR281 C. elegans dpy-5(e61) let-390(h44) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h44 arrests as a late larva or sterile adult). Maintain by picking Unc-13 and checking for correct segregation of progeny.
KR282 C. elegans let-353(h46) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplications are DpyUnc and arrest at mid-larval development. Maintain by picking Unc non-Dpy.
KR291 C. elegans let-351(h43) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplications are DpyUnc and arrest at mid-larval development. Maintain by picking Unc non-Dpy.
KR305 C. elegans let-352(h45) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h45 arrests as a late larva or sterile adult). Maintain by picking Unc-13 and checking for correct segregation of progeny.
KR332 C. elegans dhc-1(h79) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplications are DpyUnc and arrest at mid-larval development. Maintain by picking Unc non-Dpy. Previously called let-354(h79).
KR344 C. elegans let-363(h98) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the Dup are Unc. Animals which have lost the Dup are DpyUnc and arrest in late larval development. See also WBPaper00005456.
KR345 C. elegans let-361(h97) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the Dup are Unc. Animals which have lost the Dup are DpyUnc and arrest in late larval development.
KR346 C. elegans let-360(h96) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the Dup are Unc. Animals which have lost the Dup are DpyUnc and arrest in late larval development.
KR348 C. elegans let-359(h94) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h94 arrests as a sterile adult). Maintain by picking Unc-13 and checking for correct segregation of progeny.
KR350 C. elegans dpy-5(e61) let-391(h91) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h91 arrests as a late larva or sterile adult). Maintain by picking Unc-13 and checking for correct segregation of progeny.
KR352 C. elegans let-357(h89) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Strain throws Unc and DpyUncLets. DpyUncLets are sterile adults. Maintain by picking Unc.
KR356 C. elegans let-356(h83) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplications are DpyUnc and arrest at mid-larval development. Maintain by picking Unc non-Dpy.
KR358 C. elegans dpy-5(e61) let-355(h81) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplications are DpyUncLet (Sterile adults). Maintain by picking Unc non-Dpy.
KR362 C. elegans dpy-5(e61) let-388(h88) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h88 arrests as an early larva). Maintain by picking Unc-13 and checking for correct segregation of progeny.
KR363 C. elegans dpy-5(e61) let-387(h87) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h87 arrests as a mid-late larva). Maintain by picking Unc-13 and checking for correct segregation of progeny.
KR424 C. elegans dpy-5(e61) let-383(h115) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the Dup are Unc. Animals which have lost the Dup arrest as early-mid larvae.
KR427 C. elegans let-371(h123) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the Dup are Unc. Animals which have lost the Dup are DpyUnc and arrest in mid-late larval development.
KR429 C. elegans dpy-5(e61) let-381(h107) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h107 arrests as an embryo or early larva). Maintain by picking Unc-13 and checking for correct segregation of progeny.
KR431 C. elegans dpy-5(e61) let-392(h120) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the Dup are Unc. Animals which have lost the Dup are DpyUncLet and arrest in early larval development. Maintain by picking Unc.
KR433 C. elegans let-369(h125) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the Dup are Unc. Animals which have lost the Dup are DpyUnc and arrest in early larval development.
KR434 C. elegans dpy-5(e61) let-367(h119) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h119 arrests as a sterile adult). Maintain by picking Unc-13 and checking for correct segregation of progeny.
KR436 C. elegans let-372(h126) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h126 arrests as a sterile adult). Maintain by picking Unc-13 and checking for correct segregation of progeny.
KR446 C. elegans dpy-5(e61) let-376(h130) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the Dup are Unc. Animals which have lost the Dup are DpyUnc and arrest in early larval development.
KR539 C. elegans dpy-5(e61) let-393(h225) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h225 arrests as an early larva). Maintain by picking Unc-13 and checking for correct segregation of progeny.
KR548 C. elegans let-373(h234) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in early larval development.
KR557 C. elegans let-375(h241) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in mid larval development.
KR592 C. elegans let-374(h251) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h251 arrests as a mid-stage larva). Maintain by picking Unc-13 and checking for correct segregation of progeny.
KR598 C. elegans let-398(h257) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
h257 arrests in early larval development.
KR600 C. elegans let-375(h259) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h259 arrests as a leaky sterile adult). Maintain by picking Unc-13 and checking for correct segregation of progeny.
KR603 C. elegans dpy-5(e61) let-394(h262) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h262 arrests as an early to mid-stage larva). Maintain by picking Unc-13 and checking for correct segregation of progeny.
KR612 C. elegans dpy-5(e61) let-395(h271) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h271 arrests as a sterile adult). Maintain by picking Unc-13 and checking for correct segregation of progeny.
KR614 C. elegans let-399(h273) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the Dup are Unc. Animals which have lost the Dup are DpyUncLet and arrest in late larval (L4) development. DpyUncs may give some progeny that fail to grow. Maintain by picking Unc.
KR827 C. elegans let-363(h502) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication are Dpy Unc and arrest in early mid development.
OP119 C. elegans unc-119(ed3) III; wgIs119. Show Description
wgIs119 [let-381::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org).
SP142 C. elegans him-1(e879) I; mnC1 [dpy-10(e128) unc-52(e444)]/let-30(mn30) unc-4(e120) II. Show Description
Maintain strain by picking WT hermaphrodites. Segregates WT, dead eggs, paralysed DpyUnc and males.
SP143 C. elegans him-1(e879) I; mnC1 [dpy-10(e128) unc-52(e444)]/let-31(mn31) unc-4(e120) II. Show Description
Hets are WT and segregate WT, paralysed DpyUnc, L1 Lethal Unc-4s and males. Maintain by picking WT.
SP144 C. elegans him-1(e879) I; mnC1 [dpy-10(e128) unc-52(e444)]/unc-4(e120) let-32(mn32) II. Show Description
Hets are WT and segregate WT, dead eggs, paralysed DpyUnc and males. Maintain by picking WT.
SP247 C. elegans mnDp1 (X;V)/+ V; unc-3(e151) let-33(mn128) X. Show Description
NOT MALE-RESCUED MATERNAL EFFECT STERILE Maintain by picking WT.
SP253 C. elegans mnDp1 (X;V)/+ V; unc-3(e151) let-34(mn134) X. Show Description
LETHAL EARLY LARVAL. Maintain by picking WT.
SP254 C. elegans mnDp1 (X;V)/+ V; unc-3(e151) let-35(mn135) X. Show Description
Maintain by picking WT.
SP257 C. elegans mnDp1 (X;V)/+ V; unc-3(e151) let-37(mn138) X. Show Description
LETHAL EARLY LARVAL. Maintain by picking WT.
SP259 C. elegans mnDp1 (X;V)/+ V; unc-3(e151) let-36(mn140) X. Show Description
GROWTH SLOW. MALE-RESCUED. STERILE. Maintain by picking WT.
SP260 C. elegans mnDp1 (X;V)/+ V; unc-3(e151) let-38(mn141) X. Show Description
STERILE. Maintain by picking WT.
SP375 C. elegans mnDp1 (X;V)/+ V; unc-3(e151) let-39(mn144) X. Show Description
LETHAL EARLY LARVAL. Pick WT to maintain.
SP648 C. elegans let-30(mn239) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Show Description
ST9005 C. elegans ncEx9005. Show Description
ncEx9005 [lin-32p::FLAG::let-363 + lin-32p::Myc::daf-15 + lin-32p::HA::rict-1 + hsp16-2p::plx-1 + rol-6(su1006)]. Rollers. Pick Rollers to maintain. KpnI sites were added by PCR to let-363, daf-15 and rict-1 cDNAs and inserted into pPD49.26 containing lin-32p followed by the FLAG-, Myc- and HA-coding sequences to generate lin-32p::FLAG::let-363, lin-32p::Myc::daf-15 and lin-32p::HA::rict-1, respectively. Reference: Nukazuka A, et al. Nat Commun. 2011 Sep 27;2:484.
ST9007 C. elegans ncEx9007. Show Description
ncEx9007 [unc-54p::FLAG::let-363 + unc-54p::Myc::daf-15 + unc-54p::HA::rict-1 + hsp16-2p::plx-1 + rol-6(su1006)]. Rollers. Pick Rollers to maintain. KpnI sites were added by PCR to let-363, daf-15 and rict-1 cDNAs and inserted into pPD49.26 containing inserted into pPD30.38 containing unc-54p followed by the FLAG-, Myc- and HA-coding sequences to generate unc-54p::FLAG::let-363, unc-54p::Myc::daf-15 and unc-54p::HA::rict-1, respectively. Reference: Nukazuka A, et al. Nat Commun. 2011 Sep 27;2:484.
SYS668 C. elegans let-381(dev205([mNeonGreen::let-381]) I; ujIs113 II. Show Description
ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::mCherry::his-24::let-858 3Â’UTR + unc-119(+)] II. mNeonGreen knockin at N-terminus of let-381 locus. Cellular protein expression pattern during embryogenesis (until bean stage) is available at http://dulab.genetics.ac.cn/TF-atlas. Reference: Ma X, Zhao Z, Xiao L, et al. Nat Methods. 2021;18(8):893-902. doi:10.1038/s41592-021-01216-1.
UP604 C. elegans sos-1(cs41) V. Show Description
Temperature sensitive missense allele. Larval lethal and Vul at 25C. About WT at 20C. Previously called let-341.
VC2312 C. elegans let-363(ok3018) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
B0261.2. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok3018 homozygotes (late larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: TCTCCGCTCCAGCTACAAAT. External right primer: ATCGAGCTTTGCTGTTTCGT. Internal left primer: AGGGATTGATGCTGCAAGAG. Internal right primer: TTCGGAAATCGTTCCAAAAC. Internal WT amplicon: 1186 bp. Deletion size: 664 bp. Deletion left flank: GAGCCGGTATCTATCTCATCGTGTGCTTAG. Deletion right flank: ATTCGAACTCATCAGATGTGAATCCTCACA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807