JK6526 |
C. elegans |
let-711(q1238[let-711::3xV5]) III. Show Description
GSS linker and 3xV5 tag inserted at C-teminus of endogenous let-711 locus. Generated in N2 background. Reference: Carrick BH, et al. Dev Cell. 2024 Mar 11;59(5):661-675.e7. doi: 10.1016/j.devcel.2024.01.005. PMID: 38290520.
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JK6541 |
C. elegans |
gld-1(q1243) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Pick GFP+ to maintain. CRIPSR-engineered modification of gld-1 FBEa and FBEa* in gld-1 3'UTR. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q1243 homozygotes (sterility/reduced fertility). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Increase in distal GLD-1 protein levels and decrease in proximal GLD-1 protein levels. Qiu et al., in preparation.
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JK6600 |
C. elegans |
lst-1(q869) sygl-1(q1167) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Pick GFP+ to maintain. C-teminal V5 epitope tag inserted into endogenous sygl-1 locus that has a CRISPR-engineered mutation of predicted Notch-dependent cis-regulatory elementa LBS BCD (Yoo et al., 2004). Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q869 q1167 homozygotes (sterility/reduced fertility). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Reference: Lynch TR, et al. Development. 2022 Apr 1;149(7):dev200332. PMID: 35394007.
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JK6602 |
C. elegans |
gld-1(q1271[*q1242]) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Engineered TGT to ACA substitutions in FBEa1 and FBEb of the endogenous gld-1 locus with a downstream G to C substitution to facilitate screening by restriction digest. Pick GFP+ to maintain. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP+, arrested hT2 aneuploids, and non-GFP q1271 homozygotes (sterile Mog). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP+ and check for correct segregation of progeny to maintain. Derived by modification of gld-1(q1242) homozygotes from parental strain JK6540. Reference: Carrick BH, et al. Dev Cell. 2024 Mar 11;59(5):661-675.e7. doi: 10.1016/j.devcel.2024.01.005. PMID: 38290520.
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JK6721 |
C. elegans |
lst-1(q1086) sygl-1(q828) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Pick GFP+ to maintain. PUF-interacting motif B (PIM B) disrupted in endogenous lst-1 locus. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q1086 q828 homozygotes (sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock.
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JK6722 |
C. elegans |
lst-1(q1124) sygl-1(q828) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Pick GFP+ to maintain. PUF-interacting motif A (PIM A) disrupted in endogenous lst-1 locus. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q1124 q828 homozygotes (sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock.
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JK993 |
C. elegans |
unc-51(e1189) fog-2(q71)/let-?(q265) V. Show Description
Heterozygotes are WT and segregate WT, Unc Females and Lethals. Maintain by picking WT. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
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JN215 |
C. elegans |
iff-1(tm483) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Segregates GFP+ glowing heterozygotes and non-glowing sterile iff-1 homozygotes. tm483 is a UV/TMP-induced iff-1 deletion allele generated by K. Gengyo-Ando and S. Mitani. hT2[qIs48] homozygotes inviable. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype.
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JS604 |
C. elegans |
dpy-17(e164) tlk-1(tm2395) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and Dpy Stu homozygotes that are non-GFP. Homozygous hT2[bli-4 let-? qIs48] inviable.
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JT5132 |
C. elegans |
+/eT1 III; exp-2(sa26)/eT1 [let-?(n886)] V. Show Description
Heterozygotes have jerky movement, are Exp defective, and are Egl (dominant). Homozygous exp-2 are recessive lethal. Homozygous eT1 are lethal also.
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JT9819 |
C. elegans |
unc-24(e138) lin-49(s1198) unc-22(s7) IV/nT1 [unc-?(n754) let-?] (IV;V). Show Description
Heterozygotes are Unc and segregate Unc, lethal Twitchers and dead eggs. Twitchers arrest in early-mid larval development. See also WBPaper00003938.
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JU1427 |
C. castelli |
Show Description
Caenorhabditis sp. 12 Male-female strain. Isolated in May 2008 from rotting Micropholis cayennensis fruit (#1, subsample L), sampled by P. Châtelet on the "Petit Plateau" near the CNRS Biological station, Nouragues, French Guyana.
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JU1428 |
C. tropicalis |
Show Description
Caenorhabditis sp. 11 Isolated by Marie-Anne Felix from rotting Duguetia surinamensis fruit, sampled by Patrick Châtelet on the "Petit Plateau" in the Nouragues Forest, French Guyana in May 2008. Hermaphrodite. Culture at 20°C or above.
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JU311 |
C. elegans |
Show Description
Isolated by Marie-Anne Felix. Merlet, Lagorce (Ardeche), France, on September 8, 2002, in wash from snail tube - snails coming from under a tree full of ivy.
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JU312 |
C. elegans |
Show Description
C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 in the wash from snail tube - snails coming from under a tree full of ivy.
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JU313 |
C. elegans |
Show Description
C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 in the wash from snail tube - snails coming from under a tree full of ivy.
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JU314 |
C. elegans |
Show Description
C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002. Snails resembling Helix, under a tree full of ivy.
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JU315 |
C. elegans |
Show Description
C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from Pomatias elegans (?) snails.
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JU316 |
C. elegans |
Show Description
C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from Pomatias elegans (?) snails.
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JU317 |
C. elegans |
Show Description
C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from destroyed snail Oxychilus sp.
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JU318 |
C. elegans |
Show Description
C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from soil under a tree full of ivy.
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JU319 |
C. elegans |
Show Description
Isolated on Sept 8, 2002, from garden soil in Merlet, Lagorce (Ardeche), France. See Barriere & Felix, Current Biology 2005 (sample Merlet 1).
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JU320 |
C. elegans |
Show Description
C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from soil under a tree full of ivy.
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JU321 |
C. elegans |
Show Description
C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from soil under a tree full of ivy.
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JU322 |
C. elegans |
Show Description
Isolated on Sept 8, 2002, from a Helix snail on the trunk of a mulberry tree in Merlet, Lagorce (Ardeche), France. See Barriere & Felix, Current Biology 2005 (sample Merlet 2).
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JU323 |
C. elegans |
Show Description
C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from a Helix (?) snail #3 on the trunk of a mulberry tree (not the same snail as JU322).
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JU342 |
C. elegans |
Show Description
C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from the wash from a snail tube (mulberry tree).
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JU343 |
C. elegans |
Show Description
C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from a Glomeris myriapod in the compost pile (same myriapod as JU344, JU345 and JU346).
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JU344 |
C. elegans |
Show Description
C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from a Glomeris myriapod in the compost pile (same myriapod as JU343, JU345 and JU346).
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JU345 |
C. elegans |
Show Description
Isolated on Sept 8, 2002, from a Glomeris myriapod in a compost pile in Merlet, Lagorce (Ardeche), France. See Barriere & Felix, Current Biology 2005 (sample Merlet 3).
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JU346 |
C. elegans |
Show Description
C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from a Glomeris myriapod in the compost pile (same myriapod as JU342, JU343 and JU344).
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JU347 |
C. elegans |
Show Description
C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from a Pomatias elegans (?) snail, under the mulberry tree.
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JU348 |
C. briggsae |
Show Description
From Merlet, Lagorce (Ardèche), France. 8 Sep 02. From a Oxychilus sp. snail , under the mulberry tree.
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KB4 |
C. elegans |
glh-4(gk225) glh-1(ok439) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Pick GFP+ to maintain balanced stock. Heterozygotes are superficially wild-type GFP+ and segregate wild-type GFP+ (heterozygotes), arrested hT2 aneuploids, and non-GFP glh-4 glh-1 homozygotes (sterile; incompletely penetrant). NOTE (K. Bennett, 2012): KB4 strain is only 63% sterile at 20C and 92% sterile at 26C (Spike et al., Genetics 2008 178:1973). glh-1(ok439) is not a null allele.
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KG1180 |
C. elegans |
lite-1(ce314) X. Show Description
Defective response to short wavelength light; response strongly reduced but not eliminated. All other characteristics seem wild type, including reponse to mechanosensory stimuli. Strong, probably null, allele. This mutation also blocks the coordinated light response of unc-31(e928) and egl-30(ad805). To identify lite-1 homozygous mutants when crossing into different backgrounds, use a fluorescence stereomicroscope with a GFP filter and zoom to the hightest magnification (60-100X) to distinguish Lite from non-Lite animals. This works best when the animals are mired in thicker parts of the food to slow their spontaneous locomotion but not their response to light. Scan animals around the edge of the food where it is thickest. Leave the lid of the plate off for a minute or so before starting to let the animals adjust to air currents.
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KK288 |
C. elegans |
sqt-3(sc8) par-1(b274) V/nT1 [unc-?(n754) let-?] (IV;V). Show Description
Heterozygotes are Unc and segregate Unc, RolPar (adult homozygotes lay eggs that don't hatch) and dead eggs. nT1[unc-?(n754) let-?] is dominant Unc and recessive lethal. Strict maternal effect. sc8 previously called rol-4(sc8).
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KK299 |
C. elegans |
par-5(it55) unc-22(e66) IV/nT1 [unc-?(n754) let-?] (IV;V). Show Description
Heterozygotes are Unc and segregate Unc, Twitchers which give only dead eggs, and dead eggs. nT1 heterozygotes are shorter and slower than par-5 unc-22 homozygous worms. par-5 region is not well balanced by nT1: check to make sure that unc-22 homozygotes lay dead eggs. Strict maternal effect lethal.
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KK627 |
C. elegans |
itDf2 V/nT1 [unc-?(n754) let-?] (IV;V). Show Description
Heterozygotes are Unc and segregate Uncs and dead eggs.
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KR1315 |
C. elegans |
let-626(h676) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in late larval development.
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KR1327 |
C. elegans |
let-635(h688) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in early/late larval development.
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KR1328 |
C. elegans |
let-636(h689) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in early larval development.
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KR1329 |
C. elegans |
let-520(h690) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Unc strain which throws DpyUncLet. Late larval arrest (L4). Maintain by picking Unc non-Dpy.
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KR1331 |
C. elegans |
let-544(h692) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Unc strain which throws Unc and DpyUnc (animals which have lost the Dp). DpyUncs arrest as adults. Maintain by picking Unc.
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KR1334 |
C. elegans |
let-610(h695) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Unc-13 phenotype. Segregates Uncs and lethal DpyUncs (h695 arrests as a sterile adult). Pick Unc-13 animals to maintain. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Ann Rose.
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KR1335 |
C. elegans |
let-633(h696) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication are Dpy Unc and arrest in early larval development.
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KR1338 |
C. elegans |
let-501(h714) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in early larval development.
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KR1339 |
C. elegans |
dpy-5(e61) let-532(h715) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication are lethal DpyUncs.
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KR1341 |
C. elegans |
let-637(h700) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in mid/late larval development.
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KR1345 |
C. elegans |
let-521(h704) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Unc strain which throws Unc and DpyUncs which arrest late larval (L4). Maintain by picking Unc.
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KR1346 |
C. elegans |
let-579(h705) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in mid larval development.
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