More Fields
Strain Species Genotype
VC1274 C. elegans H20J04.6(ok1739)/mIn1 [mIs14 dpy-10(e128)] II. Show Description
H20J04.6. Homozygous sterile deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok1739 homozygotes (slow-growing, sickly, mostly sterile). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: CCCGGAGCATGAAATTCTTA. External right primer: AATGGAGCTCGAAAATGTGG. Internal left primer: TCCAACGCACAATTGAAAAA. Internal right primer: TCCAGCAAAATATGGTGCAA. Internal WT amplicon: 2146 bp. Deletion size: 853 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1275 C. elegans C47G2.5(ok1740)/mIn1 [mIs14 dpy-10(e128)] II. Show Description
C47G2.5. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok1740 homozygotes (mid-larval arrest). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: GTGGAGTGTGAAGGCCACTT. External right primer: AAAGAACCGCAAAATCGAGA. Internal left primer: AATGCACACTCTGCGTTTTG. Internal right primer: TTCTGGTTGAAAATGAGGGG. Internal WT amplicon: 3279 bp. Deletion size: 1176 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1277 C. elegans C25E10.8(ok1753) V. Show Description
C25E10.8. Superficially wild type. External left primer: GGAAGACAAAACGGGTCTCA. External right primer: AAAAGCAAAACATCGGTTGG. Internal left primer: TAACGGGCTTAAACAGACGC. Internal right primer: GGTTTCGTTCGTCATGGACT. Internal WT amplicon: 2279 bp. Deletion size: 1885 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1278 C. elegans hex-2(ok1764) V. Show Description
C14C11.3. Superficially wild type. External left primer: GAATTTCGAGGAGAGCATCG. External right primer: TTTCTTGATTGGGAAATGCC. Internal left primer: ACGTGGAGTCAGAATGTCCC. Internal right primer: GGGGACGCAGAAAAATATCA. Internal WT amplicon: 3017 bp. Deletion size: 1894 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1279 C. elegans sep-1(ok1749) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
ok1749. Homozygous sterile deletion chromosome balanced by bli-4- let-?- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok1749 homozygotes (sterile Unc adult, often with mid-body constriction). Homozygous hT2[qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GGCGTTTTGTACGTCGATTT. External right primer: TCGGATCCTACTCGCTCATT. Internal left primer: AATCGCTCCCAACAGAATTG. Internal right primer: TTATTTCAGTTCCCGGATCG. Internal WT amplicon: 2960 bp. Deletion size: 1234 bp. Deletion left flank: TTTCTCAACTTTCGGACGACGTCCGAACGG. Deletion right flank: GGAAATTGATACGTTATTTTTAAGAATGTG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1280 C. elegans kin-10(ok1751) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
T01G9.6. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok1751 homozygotes (grotty adult, dies). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CCTCGCAAAATTTCACGTTT. External right primer: TTCGACAGAAAACTGCTGGA. Internal left primer: GTGACGAAGACAGGCACAAA. Internal right primer: TTCACCCAACCTGTACCCAT. Internal WT amplicon: 2149 bp. Deletion size: 1452 bp. Deletion left flank: TCTTGAGTTTTGTCGGAAAAGAAAATTTTG. Deletion right flank: TTGAGACCTGTCAAATTGAACCGATCCTGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1282 C. elegans rabx-5(ok1763) III. Show Description
Y39A1A.5. Superficially wild type. External left primer: TGTGATGGACCAAGTGGAAA. External right primer: AACGGTGGGGGAGATAAAAA. Internal left primer: TTTTGAGGCGCTTAAGGAGA. Internal right primer: TTGAGAAAAAGCCTTTCGGA. Internal WT amplicon: 3134 bp. Deletion size: 1739 bp. Deletion left flank: GGAAAAATTGGAAATTAAATTGGAAAAAAA. Deletion right flank: TATCACTGTCCCGTCAATCATCTGAATCTG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1283 C. elegans nhr-127(gk572) V. Show Description
T13F3.3. Superficially wild type. External left primer: GTGCTCGGGAAATATTGGAA. External right primer: AATGCCCTTTCAAGGTTGTG. Internal left primer: TTTCAGGACATTTCCCGTTC. Internal right primer: GTCGAAATCTAGATCGGCCA. Internal WT amplicon: 2368 bp. Deletion size: 846 bp. Deletion left flank: TAAGTTTATAATTATGTTTAAAACTTGCCG. Deletion right flank: GAAAATTACGCTTTTCGGATTGAGAAAATG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1285 C. elegans Y43F8C.5(ok1755) V. Show Description
Y43F8C.5. Superficially wild type. External left primer: ATGCTCGGAAATCGAAAGAA. External right primer: ACATGGTTTTCTGTAGGGCG. Internal left primer: GGCAGAAGGTTGCTCATGTT. Internal right primer: GAATTCCGGCTCTTTTGTCA. Internal WT amplicon: 2345 bp. Deletion size: 1316 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1286 C. elegans Y6B3A.1(ok1736)/hIn1 [unc-101(sy241)] I. Show Description
Y6B3A.1. Apparent homozygous lethal deletion chromosome balanced by unc-101-marked inversion. Heterozygotes are WT, and segregate WT, Unc-101 hIn1 homozygotes, and ok1736 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: ATTTTCACGACGATTTTGGC. External right primer: CAGAGCGACGAAACAAGTGA. Internal left primer: CAACGCTGCGAGAATATCAA. Internal right primer: ACAATGGGTGAAAGTGAGGC. Internal WT amplicon: 2907 bp. Deletion size: 1645 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1287 C. elegans F45C12.2(gk574) II. Show Description
F45C12.2. Superficially wild type. External left primer: AAACGCTAGAGAGGCACCAA. External right primer: GATTTCGGAATGGTTCGAGA. Internal left primer: AGAGCACGAAGAAACGGAAA. Internal right primer: TCGTGAATCGTCTGAAGCTG. Internal WT amplicon: 2443 bp. Deletion size: 694 bp. Deletion left flank: CTTCAGCTGAAAACCAACTGGTTTATTTGT. Deletion right flank: AAATAACAATTGAAAAATACCCATAGCGCT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1288 C. elegans frm-3(gk585) X. Show Description
H05G16.1. External left primer: GGTCATTCTTTGGCACCAGT. External right primer: CCAAGCCGATAACCTCACAT. Internal left primer: TGAACTGAACATCTGCCAGC. Internal right primer: CTCCGCATTCCGACTGTAAT. Internal WT amplicon: 1953 bp. Deletion size: 1390 bp. Deletion left flank: GGTGTGCATCAAAGTTCGTATGCTTGATGA. Deletion right flank: GCAAAGAGATATGAACAGCTCGTTACAAAT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1289 C. elegans K09C4.5(gk571) X. Show Description
K09C4.5. External left primer: ACAGGAACTTGTCTCCCCCT. External right primer: CTTCCCAAGTGGTCGATGAT. Internal left primer: TATTGAAAGCTTCACCGGCT. Internal right primer: CAACAAATCTGGTTGGGAGG. Internal WT amplicon: 2109 bp. Deletion size: 378 bp. Deletion left flank: TCAGGTTTCGCCTCCACCGCGTTAATTTTC. Deletion right flank: CAGAGTCGCCAAAATGGCTAGTCAGACAGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1290 C. elegans nhr-125(gk578) V. Show Description
R02D1.1. External left primer: TTTCCAACTTTTTGTTCGGG. External right primer: TTACAGGTTTCAATTCCCGC. Internal left primer: GCCACGTGGAATCAAATTCT. Internal right primer: CCCTCATCAAGGTGTAAGCC. Internal WT amplicon: 1462 bp. Deletion size: 467 bp. Deletion left flank: AACATTCCATACAGATTGAATTAGTGTTAT. Deletion right flank: AGAAAGAACTTGAGCAGGCGTTTTTCAGGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1291 C. elegans nhr-227(gk580) V. Show Description
T27B7.5. Superficially wild type. External left primer: CTTCCAAAGATTGGCGAGAC. External right primer: GCAAAGTTGAGCGAACATGA. Internal left primer: AAATGGAGCTTGGTCGAGTG. Internal right primer: TCGGAGTGAGCATGAATCTG. Internal WT amplicon: 2251 bp. Deletion size: 1979 bp. Deletion left flank: AATTCATCGCCAAAAAATTTAAAAAAATCG. Deletion right flank: ATTCACTCAATTTTGATAAATTTACCAAAC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1292 C. elegans fbxa-137&nhr-228(gk581) V. Show Description
T27B7.6, T27B7.7. External left primer: CTCCCAACAACTGCCACTTT. External right primer: CATCAACCACTTTGTCACCG. Internal left primer: GCGATGGACCTGAGAGAGAA. Internal right primer: GCTTAAAGCCTTGCGTCAAC. Internal WT amplicon: 2002 bp. Deletion size: 1353 bp. Deletion left flank: CTTGGCTGTTGGTCAAATTTGAGGAGTACT. Deletion right flank: ACTTAAAAAATTACTATAAAAATGAGGGCA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1293 C. elegans F45C12.3(gk583) II. Show Description
F45C12.3. Superficially wild type. External left primer: GCTCATTGTGCACCTCCTTT. External right primer: CTGCAACAACGACTGTCCAT. Internal left primer: AGAGTGTGGAGAGCTCGGAA. Internal right primer: CCTACACCCACAGGCTGTTT. Internal WT amplicon: 1871 bp. Deletion size: 722 bp. Deletion left flank: GAGCCATATGCCGTCCGTAAAAGCGAAGAG. Deletion right flank: ACCCAGTGAGCTTGAGCTTGAAAAACTCGG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1294 C. elegans nhr-51(gk573) V. Show Description
K06B4.1. External left primer: CGAGTTGACAATCGCAAAGA. External right primer: CAAGCTTGGAGTTGAAAGCC. Internal left primer: AGCGAAACGTGTCATCAGTG. Internal right primer: CAAGCCTTTGACTTCTTGCC. Internal WT amplicon: 1850 bp. Deletion size: 446 bp. Deletion left flank: ATTCTTCCAAAGTGCAAAGCGTGTCGTTAT. Deletion right flank: TGCGAACAGGAGTTTTCCACGGAAGTATGC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1295 C. elegans R12E2.10(ok1781) I. Show Description
R12E2.10. Superficially wild type. External left primer: TGGACAGCTCGTTGTACTGG. External right primer: GAAAGGTCAGAACTCCAGCG. Internal left primer: AAGCCGCTCTAGAAACTCCC. Internal right primer: TTGTCGAGGAGACGGAGACT. Internal WT amplicon: 2457 bp. Deletion size: 1171 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1296 C. elegans F47C10(gk584) V. Show Description
F47C10. Superficially wild type. External left primer: TCCGATACTTCATGCCAACA. External right primer: ATGGTGGTTTTGTAGCAGGC. Internal left primer: AATCTGCATGATCGGCATTT. Internal right primer: ATACCTACGTGCCTGCCAAC. Internal WT amplicon: 2445 bp. Deletion size: 638 bp. Deletion left flank: GGTAGAGTACCGAAAGCTGAAACTTTGATT. Deletion right flank: ATTTGAAAACATTTATATCAACTTTTAGAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1297 C. elegans nhr-202(gk576) V. Show Description
M02H5.4. Superficially wild type. External left primer: CTCTCGCCGAGCTATTTTTG. External right primer: TCAAGTATTTGCCCGTCACA. Internal left primer: GTTGAACCTTGCGCTGAAAT. Internal right primer: TCCAATTTGAATCGATGCTG. Internal WT amplicon: 2294 bp. Deletion size: 521 bp. Deletion left flank: ACAGCAAGTCCGGGGGTCTCAGAGAAAAGT. Deletion right flank: CATCGTTGCGAGTCCCATCGTGGCGAGACG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1298 C. elegans nhr-115(gk579) V. Show Description
T27B7.4. External left primer: CCAAATTTCACTGCTCCGTT. External right primer: GAAATTGAAAAAGCGACCCA. Internal left primer: TCTTGACCGTCTGGGTCTCT. Internal right primer: GGCGCTTAACAGAACTTTGG. Internal WT amplicon: 2344 bp. Deletion size: 499 bp. Deletion left flank: TTGAAAAAAAATTTCAGTTGTTTCTGCAAA. Deletion right flank: TCTGAAAATTTGGTCAATATATTTTTAAAT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1300 C. elegans F28C6.1(gk582)/mIn1 [mIs14 dpy-10(e128)] II. Show Description
F28C6.1. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP gk582 homozygotes (early larval arrest). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: ATGATAAGACGTCCTTGCCG. External right primer: TGCCTCTGCATTGTTCTCAC. Internal left primer: TGTTTGCACTGTTCGACGTT. Internal right primer: GGCGGATTGATTCATATGCT. Internal WT amplicon: 2222 bp. Deletion size: 1146 bp. Deletion left flank: GCGGTTTTTAAAATAACGTGAATATTGCTT. Deletion right flank: GACAAACACTGAGCGAGAAAACGAATCAGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1301 C. elegans ZC506.1(ok1790) X. Show Description
ZC506.1. Superficially wild type. External left primer: TTTGCAATGTTCTGCGTCTC. External right primer: CGGCCATCACTCAAGGTAAT. Internal left primer: ACATCGTTTCTTCAATGCCC. Internal right primer: CCCCATTTCTAAGCTCTCCC. Internal WT amplicon: 2118 bp. Deletion size: 1052 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1302 C. elegans nhr-101(gk586) V. Show Description
H12C20.6. Superficially wild type. External left primer: TTCTCCCGAGACACTTGCTT. External right primer: GAAGTAGCTTTTTGGGTGCG. Internal left primer: ACCACTTGTGACACTCGCAC. Internal right primer: ATCAGCGATCCAAACAGCTT. Internal WT amplicon: 1824 bp. Deletion size: 624 bp. Deletion left flank: ATATGCTCAAAAAGCATTTGAAACATATTG. Deletion right flank: TATTCAATCTTAAAACCATAAAAATTTTTG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1303 C. elegans Y57A10A.24(ok1793) II. Show Description
Y57A10A.24. Superficially wild type. External left primer: AAAATTCCGCACCAGTAACG. External right primer: CCGTAAATTTGGCCTGAAAA. Internal left primer: GCCGAGGGACTGTAACAAGA. Internal right primer: AACAATCATCCACGTCACCA. Internal WT amplicon: 3356 bp. Deletion size: 1009 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1305 C. elegans smg-6(ok1794) III. Show Description
Y54F10AL.2. Superficially wild type. External left primer: TAGCTAGCCCATGTGCCTTT. External right primer: TTTTGCGATGTGAATCGTGT. Internal left primer: TTTTAGCCACACCATCCACA. Internal right primer: CCAAAAACATGGGAAAATCG. Internal WT amplicon: 3113 bp. Deletion size: 920 bp. Deletion left flank: CAATTAAAAATTTTTTTTCTTGATTTTCTA. Deletion right flank: AAAATTGTGTCTAGGGGTGAAAAATTGCGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1306 C. elegans VC5.2(ok1796) V. Show Description
VC5.2. Superficially wild type. External left primer: ATTGAACGCGTCACATCAAA. External right primer: ATTCGAGGGGGAAATACCAC. Internal left primer: AGAGAAGACGGTTTGACCCA. Internal right primer: CAAATTTTAGGGAGACGCCA. Internal WT amplicon: 3163 bp. Deletion size: 1645 bp. Deletion left flank: GCAAATTTATCAGTTGCATATGCTTTTGGA. Deletion right flank: AAATTTTTAAAATGGTTCTAGAAAGCTTAC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1307 C. elegans F52E4.1(ok1686) X. Show Description
F52E4.1. Superficially wild type. External left primer: AAGAACCTTGATTCGCAGGA. External right primer: GAGTGGAATGTTTCCGTGCT. Internal left primer: CCGTTGAGAACCGATTTGAT. Internal right primer: GTTCAAATCCTCGCACACCT. Internal WT amplicon: 2518 bp. Deletion size: 1301 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1308 C. elegans eef-2(ok1774) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
F25H5.4. Apparent homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok1774 homozygotes (arrest stage/phenotype undetermined). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: TTGGCGCTCTACCGTACTTT. External right primer: AGGCCGAAACAAATTCAATG. Internal left primer: GCAAATTTTGGGCCTACTGA. Internal right primer: AAACGATCTGGTTTGGCTTG. Internal WT amplicon: 2855 bp. Deletion size: 1443 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1309 C. elegans nlp-8(ok1799) I. Show Description
D2005.2. Superficially wild type. External left primer: TCGGAAATGATTCATAGGGC. External right primer: TCACACCTCATACCCCCATT. Internal left primer: CTTTCAAATCACCCGACCAT. Internal right primer: TTCTTGATCTACCCGAACCG. Internal WT amplicon: 2229 bp. Deletion size: 695 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1310 C. elegans cey-1(ok1805) II. Show Description
F33A8.3. Superficially wild type. External left primer: CCGTTTCTCGAAAGTGCTTC. External right primer: TACACTGACCGCTGCTCATC. Internal left primer: AACCGGAGAAGGAGAAGCTC. Internal right primer: GGTCAGCTTACACACTCGCA. Internal WT amplicon: 2614 bp. Deletion size: 539 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1311 C. elegans cpr-3(ok1788) V/nT1 [qIs51] (IV;V). Show Description
T10H4.12. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok1788 homozygotes (mildly Unc, mid-larval arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: AAACAAATCCACCCTGCATC. External right primer: TCGATTCACCACTGTTTTGC. Internal left primer: CATTGCTCGTCATGTTGGTT. Internal right primer: CCTCAACGCTTTGATAAGCC. Internal WT amplicon: 2200 bp. Deletion size: 1225 bp. Deletion left flank: CACTGCGGATCTGGGATTCAGGTACGCTGT. Deletion right flank: GTGTCACCGAATCAAGAAATCAATTATCAC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1312 C. elegans ift-81(gk512) X. Show Description
F32A6.2. External left primer: TGCCTACTTCCAACGCTTTT. External right primer: AACTTCGGCTGTCTTGCCTA. Internal left primer: CAACAAGTCGATGCCTGAAA. Internal right primer: GCGCTCTTTCAAACCTTCTG. Internal WT amplicon: 1863 bp. Deletion size: 269 bp. Deletion left flank: TCAAGGGTTTATTCTCCACTTTTTGAATGA. Deletion right flank: TAAATTTTTTTAATTTCTAAAAATAGCTTT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1313 C. elegans par-2(ok1723)/sC1 [dpy-1(s2170)] III. Show Description
F58B6.3. Apparent homozygous lethal deletion chromosome balanced by dpy-1-marked recombination suppressor. Heterozygotes are WT, and segregate WT, Dpy (sC1 homozygotes), and ok1723 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: AAATCACACCGATTTCTGCC. External right primer: AAAATTGGTGCGAAAACGAC. Internal left primer: AGCGGAGTTTTCCGATTTTT. Internal right primer: AAAAAGGCTACGAAACGGGT. Internal WT amplicon: 2390 bp. Deletion size: 1110 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1314 C. elegans F53B2.3(ok1783) IV. Show Description
F53B2.3. Superficially wild type. External left primer: AATACCGACGCATCAGGAAG. External right primer: CGTTAGTGTGGGCTTTGGAT. Internal left primer: TGGCTCATCATCAGACTTGG. Internal right primer: GATTCATGAGAGGTCTCGCC. Internal WT amplicon: 2254 bp. Deletion size: 929 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1315 C. elegans T10F2.4(gk575) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
T10F2.4. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP gk575 homozygotes (sterile with no eggs). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: AACACGTCACCAATGAACGA. External right primer: CGTGGTGTCTCGAATTCCTT. Internal left primer: CATGTCGAAGGACAGGGAGT. Internal right primer: TTCGTTTATGTCCCACGTCA. Internal WT amplicon: 1565 bp. Deletion size: 632 bp. Deletion left flank: TGAATGTTTGCATAACCTGTTCCTTTTCAT. Deletion right flank: AGATAATTGCAAGGTTGACAAACCTGGTCT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1316 C. elegans bbs-5(gk537) III. Show Description
R01H10.6. Superficially wild type. External left primer: ACTTCGGTAAATCGACACCG. External right primer: TGTCCGAAGTGGTTTCATCA. Internal left primer: TTCGAGACGGTAAATCAGCC. Internal right primer: GAACCTACTCGCAGGGTGTC. Internal WT amplicon: 1513 bp. Deletion size: 692 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1317 C. elegans lem-2(ok1807) II. Show Description
W01G7.5. Superficially wild type. External left primer: CGACCGAAATCACGAGAAAT. External right primer: CAGACGGAAGCACAAGAACA. Internal left primer: TCGAGACTCGCAGAGGATTT. Internal right primer: GTAGCGACGCGAGACTCATT. Internal WT amplicon: 2741 bp. Deletion size: 2169 bp. Deletion left flank: CTTAATTTCCGGTGAGATATTCTGTAGTTT. Deletion right flank: CTTTTCCTCGTCCCTCGTGAGGAAAAAGTTGCAGTGTCGG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1318 C. elegans Y65B4A.2(ok1809) I. Show Description
Y65B4A.2. Superficially wild type. External left primer: TGATGACTTCCACACGGTTC. External right primer: CGTTCAGCTTCTCGGTTTTC. Internal left primer: TTCGACAAAACATGGTGCAT. Internal right primer: TACTCGTTCCTTTCCATCGG. Internal WT amplicon: 3406 bp. Deletion size: 1338 bp. Deletion left flank: TATCGATTTTTTCCGATAATTATCGATTTT. Deletion right flank: TCCGATAATAATCGACTTTTCCGATAGTTA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1319 C. elegans nhr-96(gk589) V. Show Description
F44C8.11. Superficially wild type. External left primer: ATGTTATCCTTCGGCCTGTG. External right primer: GCTAAATTTTGGGTGGCTGA. Internal left primer: GAGCCGAAGGTTTCCTGAAT. Internal right primer: ATTGTGGGCCGCTAAATATG. Internal WT amplicon: 1693 bp. Deletion size: 339 bp. Deletion left flank: TTACTCTTATATTTGGGCCTGCGCATTCCA. Deletion right flank: AAGTTTTTTGAGGGCTGCAGAGTGGTTTTC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1320 C. elegans nhr-123(gk577) V. Show Description
M02H5.7. Superficially wild type. External left primer: CTGCGAAGAGGTGTGAGACA. External right primer: TGATGGAAGCATAATTGGCA. Internal left primer: ATTCGAAATCGGAGAGCAGA. Internal right primer: ATTGGTCGAAACTGGAATGC. Internal WT amplicon: 2189 bp. Deletion size: 427 bp. Deletion left flank: GGTATTTCAAAAAAATTCAAGCCTTGCAAA. Deletion right flank: CAAGTTCCCAATGTGTTGCAAAAATTAGCG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1321 C. elegans nhr-16(gk588) II. Show Description
T12C9.6. External left primer: TTCCTGCCAGGGAAATAATG. External right primer: AAAGTCGTGGATCCGAGTTG. Internal left primer: CTTACCTACCGCCTGCTTTG. Internal right primer: GACGAGTATCCTAGCGGCTG. Internal WT amplicon: 2095 bp. Deletion size: 963 bp. Deletion left flank: TTTTATCTATTTAATATTCACAAGGGTGTA. Deletion right flank: ATAATATGTAGAAGCTTTTGTAAGGTGTGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1322 C. elegans nhr-16(gk587) II. Show Description
T12C9.6. External left primer: TTCCTGCCAGGGAAATAATG. External right primer: AAAGTCGTGGATCCGAGTTG. Internal left primer: CTTACCTACCGCCTGCTTTG. Internal right primer: GACGAGTATCCTAGCGGCTG. Internal WT amplicon: 2095 bp. Deletion size: 559 bp. Deletion left flank: TTACATCAAATGCATCGAAGTTGGAATGAA. Deletion right flank: TTGTTCAGAGTCGGATAGGCAAATTTCAAG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1323 C. elegans nhr-127(gk593) V. Show Description
T13F3.3. Superficially wild type. External left primer: GTGCTCGGGAAATATTGGAA. External right primer: AATGCCCTTTCAAGGTTGTG. Internal left primer: TTTCAGGACATTTCCCGTTC. Internal right primer: GTCGAAATCTAGATCGGCCA. Internal WT amplicon: 2368 bp. Deletion size: 1608 bp. Deletion left flank: TCCCTGCGAAGTGTGCAAAAATCAATCAAA. Deletion right flank: TTTTTGGAACAGATGTTTAATCATATGGCC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1325 C. elegans tbg-1&F58A4.9(ok1786) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
F58A4.9, F58A4.8. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok1786 homozygotes (probable embryonic arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GTTCGTTGTTGCGGAATTTT. External right primer: CGCCGAGTTCAAAAAGAAAG. Internal left primer: CTTTCTTCGGAAATGCTTCG. Internal right primer: GCTCAATGTGTTCGCAGAAG. Internal WT amplicon: 2187 bp. Deletion size: 1269 bp. Deletion left flank: GGCTTGAGCAAGCTGATTTCCGCACTGTCC. Deletion right flank: GAGTAAGAAAAAGAAGATCAAAGTGGATAG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1326 C. elegans VC5.2(ok1797) V. Show Description
VC5.2. Superficially wild type. External left primer: ATTGAACGCGTCACATCAAA. External right primer: ATTCGAGGGGGAAATACCAC. Internal left primer: AGAGAAGACGGTTTGACCCA. Internal right primer: CAAATTTTAGGGAGACGCCA. Internal WT amplicon: 3163 bp. Deletion size: 1547 bp. Deletion left flank: ACAATGAGCAGAAAAATTGCACGTGAGCCA. Deletion right flank: GTGAGTTTTAAATCGAATTTAAATTTGAAT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1328 C. elegans F09F7.5(ok1826) III. Show Description
F09F7.5. Superficially wild type. External left primer: CCCCCACACTCTCTAACCAA. External right primer: CAACTACTGAAGGCTCCCCA. Internal left primer: ACCGCCAACAATTCAGTCAT. Internal right primer: GGTTTGATCGATCGTTTGCT. Internal WT amplicon: 3139 bp. Deletion size: 1649 bp. Deletion left flank: CCAATTGTTAAAAGTACACCACCTCTCAAT. Deletion right flank: CACGTACCCAAGTTTCTCTCTGAGACTGAG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1330 C. elegans abu-14(ok1789)/mIn1 [mIs14 dpy-10(e128)] II. Show Description
ZK1067.7. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok1789 homozygotes (variable arrest, larval through sterile adult). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: CGAAAACAGAAGTTGTCGCA. External right primer: GTCAACAAACCAAATGCGTG. Internal left primer: AGAATTCAGGGAAGGGGATG. Internal right primer: CTCCGGTTTCCGAGTATGAA. Internal WT amplicon: 2113 bp. Deletion size: 292 bp. Deletion left flank: AAAAGTAGTATTTAAAAAAGAAATTTACCT. Deletion right flank: GCGTTCGAAACAACTCCTTGAATCGGAGGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1331 C. elegans acy-4(ok1806) V/nT1 [qIs51] (IV;V). Show Description
T01C2.1. Homozygous sterile deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok1806 homozygotes (sterile, lays no eggs). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: TTACATTGATTTGACCGCGA. External right primer: CGCCCGTTTCGATAAAGTAG. Internal left primer: CGACTCGCAACATTCACACT. Internal right primer: AGAGTTGGCATTCATACGGG. Internal WT amplicon: 2936 bp. Deletion size: 1318 bp. Deletion left flank: CTTAAATTCTTCCCGATCCAAAATCTGATC. Deletion right flank: ATAACACTGGTGAGAACGATGAACATTTGT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807