ZC334.9. Homozygous. Outer Left Sequence: AATTGGAGAAACTTGGCAGG. Outer Right Sequence: CAAAAGCGCCAGATAAAAGG. Inner Left Sequence: GGGTTCATTCCCTGGAAAA. Inner Right Sequence: TTTCACCCCAAATGTTCAGA. Inner Primer PCR Length: 1129 bp. Deletion Size: 584 bp. Deletion left flank: ACCTTTGTACCTACCGTCCGCCTAGGTGCC. Deletion right flank: AAGAGCACAAAGAATGAGCGCATCATATTG. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

otDf2 is a 25,484 bp deletion of the insulin cluster from Chr I, removing ins-28, ZC334.13, ins-29, ins-25, ins-27, linc-124, ZC334.12, ZC334.17, ins-24, ins-30, and ins-26. Reference: Sural S, et al. bioRxiv 2025.01.06.631508; doi: https://doi.org/10.1101/2025.01.06.631508.

wwEx78 [ins-28p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28.