| ZM7921 |
C. elegans |
lin-15B&lin-15A(n765) hpIs331 X; hpIs321; hpIs376. Show Description
hpIs331 [lgc-55Bp::tomm20::miniSOG::SL2::wCherry + lin-15(+)] X. hpIs321 [nmr-1p::tomm20::miniSOG::SL2::wCherry + lin-15(+)]. hpIs376 [unc-25p::tomm20::miniSOG::SL2::wCherry + lin-15(+)]. Maintain in the covered box to avoid unnecessary exposure to ambient light. Stimulation with blue light (460 nm LED light for 30 min at 4 Hz with 2 mW/mm2) induces mitochondrial-miniSOG ablation of AVA, AVB, AVE, AVD, RIM premotor interneurons, and D-class motor neurons. During neuron ablation, it is recommended to keep the lid of the plate open and use a heat dissipator to keep the air cool. Generated in N2 background. Reference: Gao S, et al. eLife 2018 Jan 23;7:e29915. doi: 10.7554/eLife.29915. PMID: 29360035. |
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