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Strain Species Genotype
RW12240 C. elegans hmg-20(st12240[hmg-20::TY1::EGFP::3xFLAG]) I. Show Description
CRISPR/Cas9 engineered tagged endogneous locus.
RB2143 C. elegans W02D9.3(ok2857) I. Show Description
W02D9.3. Homozygous. Outer Left Sequence: CACAATTTTCTCCTCGCGTT. Outer Right Sequence: TGTAGGCCTGATGTGGATGA. Inner Left Sequence: AAGAAGGTCCGACTCCACAA. Inner Right Sequence: GGGCTCAAAAGTGGACAAAA. Inner Primer PCR Length: 1114 bp. Deletion Size: 476 bp. Deletion left flank: AAGCCTGCACGAGAAACGCGCCAACGTCCA. Deletion right flank: AAAGTACGTAAACACCGTACACTCTACGCA. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC2340 C. elegans W02D9.3(gk1128) I; rbf-1(gk3217) III; srh-145(gk3218) V. Show Description
This strain is homozygous for a deletion (gk1128) in W02D9.3, detectable by PCR using the following primers. External left primer: ACGGATTTTGCCACTTTGTC. External right primer: CATCACATTTCTCGTGGTGG. Internal left primer: TTGGAGAGGTGTGAACGTAGAA. Internal right primer: TTTCTAGGCCGTACGTTGCT. Internal WT amplicon: 1621 bp. Deletion size: 1315 bp. Note: internal left primer binding site deleted in gk1128; major deletion product from nested PCR runs at about 650 bp. Deletion left flank: GCAGAAAAAATTTTGGAATTTGAGCTACAT. Deletion right flank: CATTTTCTTGCAGAAAAACGTGCAAAATTC. Validation: gk1128 passed by CGH. Other deletions (gk3217, gk3218) identified by CGH. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
SYS477 C. elegans hmg-20(dev129([mNeonGreen::hmg-20]) I; ujIs113 II. Show Description
ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::mCherry::his-24::let-858 3Â’UTR + unc-119(+)] II. mNeonGreen knockin at N-terminus of hmg-20 locus. Cellular protein expression pattern during embryogenesis (until bean stage) is available at http://dulab.genetics.ac.cn/TF-atlas. Reference: Ma X, Zhao Z, Xiao L, et al. Nat Methods. 2021;18(8):893-902. doi:10.1038/s41592-021-01216-1.