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Strain Species Genotype
OH96 C. elegans him-5(e1467) V; mgIs19 X. Show Description
Rollers. Throws males. mgIs19 [lim-4::GFP + rol-6(su1006)].
PS4657 C. elegans him-5(e1490) V; syIs78. Show Description
syIs78 [ajm-1::GFP + unc-119(+)] is probably on LG I (not on II, III, V or X). Not sure if unc-119(ed4) from the parent strain is still present. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
PS5970 C. elegans him-5(e1490) syIs197 V. Show Description
syIs197 [hs::LIN-3c(cDNA) + myo-2p::DsRed + pha-1(+)]. Him. Maintain at 15C. To induce LIN-3/EGF expression, heat shock at 33C for 30min (water bath) and let animals recover at least 1hr from the behavioral effects of the heat shock. Heat shock-induced LIN-3 should inhibit feeding, locomotion and sensory responses for several hours. For details on EGF-induced quiescence, see Nature Neuroscience 10, 1300 - 1307 (2007). PS5970 is identical to PS5628 as described in Development 137, 2065-2074 (2010) except that it has been outcrossed to remove accumulating suppressors.
PT2248 C. elegans pdf-1(tm1996) III; him-5(1490) V. Show Description
Male leaving assay defective (Las), lethargic, hypereversal. Reference: Barrios, A, et al. Nat Neurosci. 2012 Dec;15(12):1675-82.
PT2351 C. elegans him-5(e1490) V; myEx741. Show Description
myEx741 [pdfr-1p(3kb)::NLS::RFP + unc-122::GFP]. Him. Pick RFP+ and GFP+ to maintain. Reference: Barrios A, et al. Nat Neurosci. 2012 Dec;15(12):1675-82.
PX623 C. elegans fxDf1 II; him-5(e1490) V. Show Description
fxDf1 (II: 2,484,339 - 2,487,244) removes nspf-1, nspf-2, and nspf-3. Him. This strain carries a knockout of the Nematode-Specific Peptide family, group F (NSPF) gene family, which localizes to sperm membranous organelles. There are no effects on spermatogenesis, male fertility, or sperm competitive ability. Hermaphrodites produce approximately 30% males. Reference: Kasimatis KR, et al. (2018) BioRxiv 290221; doi: https://doi.org/10.1101/290221.
RJP255 C. elegans ynIs34 IV; him-5(e1490) V. Show Description
ynIs34 [flp-19p::GFP] IV. Him. Transcriptional flp-19 reporter. Reference: Lloret-Fernández et al. eLife 2018;7:e32785 DOI: 10.7554/eLife.32785. Clark SG & Chiu C. Development. 2003 Aug;130(16):3781-94. Kim K & Li C. J Comp Neurol. 2004 Aug 2;475(4):540-50.
ST53 C. elegans ncIs3 III; him-5(e1490) V. Show Description
ncIs3 [pH20::GFP + pBlueScript]. Expresses GFP in nearly all neurons. No morphological or behavioral phenotypes.
AD281 C. elegans spe-45(as38) IV; him-5(e1490) V. Show Description
Him. Temperature-sensitive sterile. Small brood size even at permissive temperatures; pick fertile animals and maintain at 15C. Worms lacking spe-45 function produce morphologically normal and motile sperm that cannot fuse with oocytes despite direct contact in the reproductive tract. spe-45 hermaphrodites and males are subfertile at 16C and sterile at 25C. Reference: Singaravelu G, et al. Current Biology 2015. http://dx.doi.org/10.1016/j.cub.2015.10.055
AV285 C. elegans swm-1(me66) him-5(e1490) V. Show Description
Sperm activation in virgin males. Poor sperm transfer.
AV322 C. elegans swm-1(me87) him-5(e1490) V. Show Description
Sperm activation in virgin males. Very poor sperm transfer.
BA524 C. elegans fer-1(hc1) I; him-5(e1490) V. Show Description
Temperature sensitive. Maintain at 15C. Throws males.
BA547 C. elegans fer-2(hc2) III; him-5(e1490) V. Show Description
Fertile at 16c and 20C. Sterile at 25C-Produces some defective embryos and 0-5 progeny. Throws males.
BA562 C. elegans fer-4(hc4) him-5(e1490) V. Show Description
Temperature sensitive. Maintain at 15C. Produces about 30% males.
BA782 C. elegans spe-10(hc104) him-5(e1490) V. Show Description
Temperature sensitive, maintain at 16C. Segregates males. Average progeny at 16C is 7 +/- 5. Average progeny at 25C is 0.1 +/- .1.
BC4923 C. elegans egl-38(sy294) IV; him-5(e1490) V. Show Description
BW1809 C. elegans gpa-16(it143) I; him-5(e1490) V. Show Description
Temperature-sensitve. Maintain at 15C. Slight Maternal Effect Lethal (Mel) at 15C, more pronounced at 20C. Highly penetrant Mel at 25C and a fraction of the survivors have reversed left-right organs.
CB1490 C. elegans ali-1(e1934) him-5(e1490) V. Show Description
Faint or invisible alae in L1. Segregates males.
CB2338 C. elegans mab-6(e1249) II; him-5(e1490) V. Show Description
Segregates males. Males abnormal.
CB3297 C. elegans vab-9(e1744) II; him-5(e1490) V. Show Description
Him. M-MATING+POOR <1%WT. Tail abnormalities->knob-like swelling in larvae and adults.
CB3518 C. elegans mab-10(e1248) II; him-5(e1490) V. Show Description
Hermaphrodtes segregate impotent males. Males show very slight swelling of bursa.
CB3531 C. elegans mab-5(e1239) III; him-5(e1490) V. Show Description
Males abnormal.
CB3855 C. elegans plg-1(e2001) III; him-5(e1490) V. Show Description
Hermaphrodites and males superficially WT. Males lay down a gelatinous plug over the vulva of mated hermaphrodites. Plug formation controlled only by male genotype. Both plg-1 and plg-1/+ males make plugs.
CB3924 C. elegans pal-1(e2091) III; him-5(e1490) V. Show Description
Males are missing most of the rays and additional alae extend into the tail.
CB4043 C. elegans smg-2(e2008) I; him-5(e1490) V. Show Description
e2008 recessively suppresses the phenotypes of mutations in unc-54(r293), lin-29(n546), tra-2 and dpy-5. Abnormal male tail. smg-2 homozygotes have slightly abnormal movement.
CB4104 C. elegans mab-12(e2166) IV; him-5(e1490) V. Show Description
Males have abnormal swollen bursa. Hermaphrodites have abnormal swollen vulva.
CB4111 C. elegans mab-24(e2169) I; him-5(e1490) V. Show Description
Anterior displacement of rays and fan. Ray identities are not altered. Slightly Unc (coiler).
CB4219 C. elegans pal-2(e2260) IV; him-5(e1490) V. Show Description
WT hermaphrodites. Mails have ectopic lateral alae in tail region. Segregates males.
CB4354 C. elegans smg-3(ma117) IV; him-5(e1490) V. Show Description
Hermaphrodites have an abnormal protrusive vulva. Segregate males with abnormally swollen bursa. Suppressor of certain alleles of certain genes.
CB4605 C. elegans mab-9(e2410) II; him-5(e1490) V. Show Description
CB5365 C. elegans tra-3(bn75) IV; him-5(e1490) V. Show Description
Him. Temperature-sensitive: infertile above 20C. Variably masculinized XX hermaphrodites segregating normal XO males. Stronger XX masculinization than null alleles of tra-3. Reference: Francis et al. (1995) PMID: 7713420.
CB6740 C. elegans bus-20(e2990) III; him-5(e1490) V. Show Description
Anatomically normal hermaphrodites and males; Bus (resistant to infection by M. nematophilum); weakly resistant to Leucobacter Verde2; insensitive to Leucobacter Verde1. Reference: J Hodgkin (unpublished).
CB6742 C. elegans dhs-5(e2992) II; him-5(e1490) V. Show Description
Bus (resistant to M. nematophilum infection); sensitive to Leucobacter Verde2, hypersensitive to Leucobacter Verde1. Reference: M J Gravato-Nobre, J Hodgkin (unpublished).
CB7109 C. elegans acs-20(e3031) IV; him-5(e1490) V. Show Description
Him. Skiddy, bleach-sensitive hermaphrodites and males. Resistant to Leucobacter Verde1. Reference: O'Rourke et al. (in preparation).
CB7148 C. elegans him-5(e1490) V; bus-8(tm1410) X; eEx763. Show Description
eEx763 [bus-8(exonU2stop) + sur-5p::GFP]. Pick GFP+ to maintain. Lethal bus-8 mutation rescued by bus-8 transgene defective in 5' exons U1 and U2. Reference: Stroud et al (in preparation).
CB7425 C. elegans him-5(e1490) V; bus-25(e3094) X. Show Description
Him. Small, slow-growing, bleach-sensitive; Bus (resistant to M. nematophilum), slightly resistant to Leucobacter Verde2, hypersensitive to Leucobacter Verde1. Reference: Hodgkin et al (in preparation).
CF250 C. elegans cat-4(e1141) him-5(e1490) V. Show Description
Catecholamine abnormal. Throws males.
CF26 C. elegans lin-22(mu2) IV; him-5(e1490) V. Show Description
Alae-to-ray transformation similar to that of n372 animals.
CF31 C. elegans lin-22(mu5) IV; him-5(e1490) V. Show Description
Alae-to-ray transformation similar to that of n372 animals.
CF32 C. elegans lin-1(e1026) IV; him-5(e1490) V. Show Description
Very slow growth.
CF491 C. elegans pry-1(mu38) I; him-5(e1490) V. Show Description
Very sick, Muv, Scrawny. Extra rays in males in body. Ectopic expression of lin-39, mab-5, egl-5. Cold sensitive - grows better at 25C.
CF80 C. elegans mab-3(mu15) II; him-5(e1490) V. Show Description
Abnormal V rays (male).
CG1 C. elegans lev-11(rg1) I; him-5(e1490) V. Show Description
Resistant to levamisole. Egl-d. Twitch at 25C. Long. Throws males.
CG118 C. elegans unc-43(sy574) IV; him-5(e1490) V. Show Description
Males constitutively protract their spicules in the absence of mating cues.
CG197 C. elegans egl-2(rg4) him-5(e1490) V. Show Description
No longer able to suppress male muscle seizures in the absence of food.
CG21 C. elegans egl-30(tg26) I; him-5(e1490) V. Show Description
Hyperactive. Small. Spicule protruder. Coiler. Throws males.
CL183 C. elegans him-5(e1490) srf-4(ct109) V. Show Description
Animals commonly have a protruding vulva. Unc (slow moving and non-sinusoidal body posture). Egl. Ectopic surface binding of the lectins WGA and SBA. Males are infertile and Mab-crumpled spicules and abnormal rays and lack diagonal sex muscles.
CL2008 C. elegans him-5 (e1490) V; dvIs3. Show Description
dvIs3 [unc-54p::human transthyretin + rol-6(su1006)]. Rollers. Him. Expression of wild-type human transthyretin (TTR) in body wall muscles. Transthyretin is secreted from the muscles into the pseudocoelom and concentrates in the coelomocytes. Reference: Link CD (1995) Proc Natl Acad Sci U S A 92:9368-9372.
CL208 C. elegans srf-9(dv4) him-5(e1490) V. Show Description
Animals are somewhat smaller than WT and commonly have a protruding vulva. Unc(slow moving and non-sinusoidal body posture). Egl. Ectopic surface binding of the lectins WGA and SBA. Males are infertile and Mab.
CL261 C. elegans him-5(e1490) V; srf-5(ct115) X. Show Description
WT except for ectopic surface binding of wheat germ agglutinin (WGA).