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Strain Species Genotype
DF64 C. elegans nyDf1(ny15) IV; him-5(e1490) V. Show Description
Deficiency covers at least all of cosmid T23G4; right breakpoint may be in cosmid C47A4. Fails to complement tlp-1(bx85). Viable and fertile. Although hermaphrodites appear WT in other ways, there are some problems with T cell lineages (affecting the phasmids) and tail cell fusions. Variably Dyf. Male tail tip morphogenesis is also defective, resulting in blobby, "leptoderan" tails. Males are infertile due to an inability to properly copulate.
DS88 C. elegans emb-27(ax81) II; him-5(e1490) V. Show Description
Temperature sensitive. Maintain at 15C.
EG8073 C. elegans oxIs322 II; oxSi199 IV; oxTi81 him-5(e1490) V. Show Description
oxIs322 [myo-2p::mCherry::H2B + myo-3p::mCherry::H2B + ? + Cbr-unc-119(+)] III. Nuclear, red fluorescence in pharynx and body wall muscle. Complex integration into ttTi5605 mosSCI site (II:0.77). oxSi199 [unc-57p::tdTomato + unc-119(+)] IV. Synaptic red fluorescence visible on fluorescence dissecting scope. Integration into cxTi10882 mosSCI site (IV:-0.05). oxTi81 [eft-3p::GFP::H2B::tbb-2 3'UTR + unc-18(+)] V. Nuclear, green fluorescence is broadly expressed (in most cells). Integration into chr.V: 1.21. Him. Combined fluorescent balancer strain for LG II, LG IV and LG V. Strain contains him-5(e1490) to generate males for crosses.
EG8397 C. elegans oxIs322 II; oxTi80 III; him-5(e1490) V. Show Description
oxIs322 [myo-2p::mCherry::H2B + myo-3p::mCherry::H2B + ? + Cbr-unc-119(+)] III. Nuclear, red fluorescence in pharynx and body wall muscle. Complex integration into ttTi5605 mosSCI site (II:0.77). oxTi80 [eft-3p::GFP::H2B::tbb-2 3'UTR + unc-18(+)] III. Nuclear, green fluorescence is broadly expressed (in most cells). Integration into chr. III: 21.21. Him. Combined fluorescent balancer strain for LG II and LG III. Strain contains him-5(e1490) to generate males for crosses.
EG8398 C. elegans oxIs322 II; oxTi80 III; oxSi199 IV; him-5(e1490) V. Show Description
oxIs322 [myo-2p::mCherry::H2B + myo-3p::mCherry::H2B + ? + Cbr-unc-119(+)] III. Nuclear, red fluorescence in pharynx and body wall muscle. Complex integration into ttTi5605 mosSCI site (II:0.77). oxTi80 [eft-3p::GFP::H2B::tbb-2 3'UTR + unc-18(+)] III. Nuclear, green fluorescence is broadly expressed (in most cells). Integration into chr. III: 21.21. oxSi199 [unc-57p::tdTomato + unc-119(+)] IV. Synaptic red fluorescence visible on fluorescence dissecting scope. Integration into cxTi10882 mosSCI site (IV:-0.05). Him. Combined fluorescent balancer strain for LG II, LG III and LG IV. Strain contains him-5(e1490) to generate males for crosses.
EG8760 C. elegans oxIs322 II; oxTi79 III; him-5(e1490) V. Show Description
oxIs322 [myo-2p::mCherry::H2B + myo-3p::mCherry::H2B + ? + Cbr-unc-119(+)] III. Nuclear, red fluorescence in pharynx and body wall muscle. Complex integration into ttTi5605 mosSCI site (II:0.77). oxTi79 [eft-3p::GFP::H2B::tbb-2 3' UTR + unc-18(+)] III. Nuclear, green fluorescence is broadly expressed (in most cells). Integration into chr. III: -26.22. Him. Combined fluorescent balancer strain for LG II and LG III. Strain contains him-5(e1490) to generate males for crosses.
EM101 C. elegans lin-41(bx37) I; him-5(e1490) V. Show Description
Maintain under normal conditions. Males have long tail tips.
EM105 C. elegans mab-21(bx41) III; him-5(e1490) V. Show Description
Transformation of ray 6 to a thin ray which is anteriorly displaced and fuses with ray 4 (95%). A 10th ray is found in about 50% of the sides scored. Body is slightly shorter.
EM106 C. elegans lin-41(bx42) I; him-5(e1490) V. Show Description
Maintain under normal conditions. Males have long tail tips.
EM111 C. elegans him-5(e1490) V; mab-19(bx38) X. Show Description
Male phenotype: loss of rays 7-9; incomplete penetrance/expressivity (80%); T lineage defect. Hermaphrodite phenotype: lowered brood size (100 progeny/hermaphrodite). Hypomorphic allele: uDf1/mab-19(bx38) results in embryonic arrest during morphogenesis.
EM116 C. elegans mab-25(bx27) I; him-5(e1490) V. Show Description
Temperature sensitive. Missing Ray. Swollen tail and reduced fan. Temperature sensitive lethal at all stages. Wrinkled spicule.
EM128 C. elegans mab-21(bx53) III; him-5(e1490) V. Show Description
Transformation of ray 6 to a thin ray which is anteriorly displaced and fuses with ray 4 (95%). A 10th ray is found in about 50% of the sides scored. Slightly shorter than WT. mab-21(bx53)/yDf10 is embryonically lethal with embryo arrest at 2 fold stage.
EM131 C. elegans ram-2(bx39) II; him-5(e1490) V. Show Description
Lumpy rays.
EM139 C. elegans ram-1(bx34) I; him-5(e1490) V. Show Description
Lumpy rays.
EM207 C. elegans tbx-2(bx59) III; him-5(e1490) V. Show Description
Conditional lethal: inviable when grown at 25C. Wild type at 16C. Viable when grown at 20C; partial ray loss at 20C. Shifting males during ray assembly to non-permissive temperature results in ray loss. No lineage defect.
EM253 C. elegans mab-20(bx61) I; him-5(e1490) V. Show Description
Ray 3 and 4 fusion >60% at non-permissive temp. Ray 1 and 2 fusion about 10% at non-permissive temp. ts period is around L3-L4.
EM305 C. elegans efn-4(bx80) IV; him-5(e1490) V. Show Description
Extensive ray fusion involving all 9 rays. Larva have Vab phenotype with decreasing expressivity in adult. Hermaphrodites have swollen tail and anus. bx80 pka mab-26(bx80).
EM331 C. elegans him-5(e1490) V; mab-19(bx83) X. Show Description
Male phenotype: loss of rays 7-9; incomplete penetrance/expressivity (80%); T lineage defect. Hermaphrodite phenotype: lowered brood size (100 progeny/hermaphrodite). Hypomorphic allele: uDf1/mab-19(bx83) results in embryonic arrest during morphogenesis.
EM347 C. elegans tlp-1(bx85) IV; him-5(e1490) V. Show Description
Although hermaphrodites appear WT in other ways, there are some problems with T cell lineages (affecting the phasmids) and tail cell fusions. Variably Dyf. Male tail tip morphogenesis is also defective, resulting in blobby, "leptoderan" tails. Males are infertile due to an inability to properly copulate. tlp-1 encodes a nuclear protein with a single C2H2-type zinc finger domain and an N-terminal "SPLALLA" domain, similar to that of Sp1 transcription factors of vertebrates. The bx85 mutation involves a truncation of TLP-1 due to a frameshift caused by a 5-bp deletion.
EM66 C. elegans him-5(e1490) V; vab-3(bx23) X. Show Description
Transformation of ray 6 to a thin ray which is anteriorly displaced and fuses with ray 4 (99%). Body is slightly shorter. See also WBPaper00002235.
EM67 C. elegans mab-20(bx24) I; him-5(e1490) V. Show Description
Extensive ray fusion. Posterior portion of body swollen at larval stages.
EM68 C. elegans col-34(bx25) IV; him-5(e1490) V. Show Description
Lumpy rays. Temperature sensitive. bx25 previously called ram-4.
EM723 C. elegans sop-2(bx91) II; him-5(e1490) V. Show Description
Temperature sensitive. Early larval arrest at 25C. At 20C, Lon, PVul, Muv, Mog, ectopic Hox gene expression.
EM81 C. elegans him-5(e1490) V; ram-5(bx30) X. Show Description
Lumpy rays.
EM938 C. elegans pdfr-1(bx142) III; him-5(e1490) V. Show Description
Male leaving assay defective (Las), lethargic, hypereversal. Reference: Barrios, A, et al. Nat Neurosci. 2012 Dec;15(12):1675-82.
EM97 C. elegans ram-2(bx32) II; him-5(e1490) V. Show Description
Lumpy rays.
EU1472 C. elegans let-99(or204) IV; him-5(e1490) V. Show Description
let-99(or204) is temperature-sensitive, maternal-effect, embryonic-lethal. Nearly completely penetrance of lethality at 26C. Viable at 15C. Maintain at 15C. Reference: Goulding MB, et al. J Cell Biol. 2007 Sep 24;178(7):1177-91.
HA2464 C.elegans sod-1(tm776) II; rtSi8 IV; him-5 V; vsIs48. Show Description
rtSi8 [sod-1p::sod-1(A4V) + Cbr-unc-119(+)] (inserted into cxTi10882) IV. vsIs48 [unc-17::GFP]. A4V mutation in C. elegans sod-1 genomic rescue construct mimics human SOD1 disease model. Superficially wild-type with increased sensitivity to paraquat in multiple assays. GFP expressed in all cholinergic neurons. Strain reportedly carries a him-5 mutation in the background, though specific allele has not been confirmed. HA2619 serves as a control strain for HA2464. Reference: Baskoylu SN, et al. PLoS Genet. 2018;14(10):e1007682.
HS144 C. elegans msi-1(os1) III; him-5(e1490) V. Show Description
1.6 kb deletion in msi-1. Males have poor mating efficiency.
HY618 C. elegans mat-2(or374) II; him-5(e1490) V. Show Description
Temperature-sensitive embryonic lethal, maintain at 15C. Shift L4 hermaphrodites to 25C overnight for mutant embryos. Mutant embryos are sensitive to osmolarity and pressure. Previously called pod-3(or374).
HY619 C. elegans mat-2(or385) II; him-5(e1490) V. Show Description
Temperature-sensitive embryonic lethal, maintain at 15C. Shift L4 hermaphrodites to 25C overnight for mutant embryos. Mutant embryos are sensitive to osmolarity and pressure. Previously called pod-3(or385).
HZ1683 C. elegans him-5(e1490) V; atg-2(bp576) X. Show Description
Him; ~30% male.
HZ1684 C. elegans atg-3(bp412) IV; him-5(e1490) V. Show Description
Him; ~30% male.
HZ1687 C. elegans atg-9(bp564) him-5(e1490) V. Show Description
Him; ~30% male.
HZ1690 C. elegans epg-6(bp242) III; him-5(e1490) V. Show Description
Him; ~30% male.
HZ1691 C. elegans epg-8(bp251) I; him-5(e1490) V. Show Description
Him; ~30% male.
JK2505 C. elegans cyd-1(q626) II; him-5(e1490) V. Show Description
Temperature-sensitive allele of cyd-1. Phenotypically wild-type at 15C. At 25C, approximately one-third of q626 hermaphrodites were missing one distal tip cell (DTC) and approximately one-half of q626 males were missing the linker cell (LC). q626 also feminizes the XO gonad. q626 affects the production of SGP daughters in both sexes. There is also a maternal component since the mutant phenotype is almost fully penetrant in offspring of homozygous mothers, but less penetrant in offspring of heterozygous mothers. Reference: Tilmann C & Kimble J. Dev Cell. 2005 Oct;9(4):489-99.
JT8132 C. elegans lin-49(sa470) IV; him-5(e1490) V. Show Description
Males are abnormal. Variable additional defects: Con, Egl, Unc. Temperature sensitive. See also WBPaper00003938.
JT8193 C. elegans lin-59(sa489) I; him-5(e1490) V. Show Description
Males are abnormal. Variable additional defects: Con, Egl, Unc. Temperature sensitive. See also WBPaper00003938.
JT9461 C. elegans lin-48(sa469) III; him-5(e1490) V. Show Description
Male spicule defect.
KC565 C. elegans sin-3(tm1276) I; him-5(e1490) V. Show Description
Mab. Pvl. Throws males.
MH1157 C. elegans him-5(e1490) V; egl-13(ku194) X. Show Description
ku194 is a loss of function allele, likely to be molecular null. Connection of gonad defective, >95% Egl. Anchor cell and uterine seam cell do not fuse. Males can mate. Hermaphrodites are very difficult to mate. Previously called cog-2(ku194).
MH1946 C. elegans dli-1(ku266)/+ IV; him-5(e1490) V. Show Description
Heterozygotes are WT and segregate WT and Sterile animals with a protruding vulva. Throws males. Clone several WT to recover heterozygote. Cytoplasmic dynein light intermediate chain. 9/02: ku266 is not an L to stop; it is a W117 to a stop (TGG to TGA). Sandhya Koushika.
MT1329 C. elegans lin-12(n302) III; him-5(e1467) V. Show Description
Vulvaless. Segregates males which mate well.
MT2021 C. elegans lin-12(n952) III; him-5(e1467) V. Show Description
Muv. Throws males.
MT4051 C. elegans lin-44(n1792) I; him-5(e1490) V. Show Description
At 20C about 50% of the hermaphrodites are Egl. Males have an abnormal tail and do not mate.
MT4718 C. elegans egl-31(n472) I; him-5(e1490) V. Show Description
Egg laying defective. Makes bags of worms. Backward Unc. Throws males.
MT491 C. elegans him-5(e1467) V; lin-2(e1309) X. Show Description
Vulvaless. Segregates males.
MT529 C. elegans him-5(e1467) V; lin-14(n179) X. Show Description
Abnormal vulva (temperature sensitive). Segregates males.
MT633 C. elegans lin-11(n389) I; him-5(e1467) V. Show Description
Vulvaless. Slightly Unc. Throws males.