| AMH46 |
C. elegans |
juIs76 II; daf-2(e1370) III; unc-33(e1193) IV. Show Description
Maintain at 15C. Synthetic Lethality at 25C. Unc; almost paralyzed. Growth slow.
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| KR282 |
C. elegans |
let-353(h46) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplications are DpyUnc and arrest at mid-larval development. Maintain by picking Unc non-Dpy.
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| KR795 |
C. elegans |
let-600(h468) dpy-5(e61) unc-13(e450) I; sDp2 (I;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in late larval development.
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| OH4605 |
C. elegans |
unc-37(ot59)/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); him-8(e1489) IV; otIs3 V. Show Description
Heterozygotes are WT and GFP+ in the pharynx. ot59 is homozygous inviable. qIs48 is an insertion of ccEx9747 (carries myo-2::GFP, pes-10::GFP, and a gut enhancer fused to GFP) onto the hT2 chromosome and is homozygous lethal. otIs3[lin-15(+) + gcy-7::GFP]. otIs3 is expressed in ASEL in WT animals. In this ot59 strain, otIs3 is expressed in ASEL and ASER.
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| OH4609 |
C. elegans |
otEx2646. Show Description
otEx2646 [ceh-36::GFP::unc-54 3' UTR + rol-6(su1006)]. Maintain by picking Rollers.
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| OH4610 |
C. elegans |
otEx2647. Show Description
otEx2647 [ceh-36p2::GFP::cog-1 3'UTR + rol-6(su1006)].
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| OH4617 |
C. elegans |
otEx2654. Show Description
otEx2654 [ceh-36p2::GFP::cog1 3' UTR + rol-6(su1006)].
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| TH463 |
C. elegans |
unc-119(ed3) III; ddIs263. Show Description
ddIs263 [eat-4::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Cell. 2012 Aug 17;150(4):855-66. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)
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| WH468 |
C. elegans |
sep-1(e2406) I/hT2 (I;III); ruIs32 III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Homozygous embryonic lethal mutation balanced by bli-4- and GFP-marked translocation. Maintain at 15 C. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP e2406 homozygotes (embryonic arrest). Homozygous hT2 [bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Bembenek J, et al. Curr Biol (2010) Feb 9;20(3):259-64.
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| YH461 |
C. elegans |
ifta-2(tm1724) IV. Show Description
Extended life span. daf-d.
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