More Fields
Strain Species Genotype
IK800 C. elegans gcy-8(oy44) IV. Show Description
Nearly normal thermotaxis behavior.
PY1322 C. elegans oyIs18 X. Show Description
oyIs18 [gcy-8::GFP]. GFP in AFD.
IK597 C. elegans gcy-23(nj37) gcy-8(oy44) gcy-18(nj38) IV. Show Description
Severe defects in thermotaxis.
DA1267 C. elegans lin-15B&lin-15A(n765) X; adEx1267. Show Description
adEx1267 [gcy-8::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive.
GN112 C. elegans pgIs2. Show Description
pgIs2 [gcy-8p::TU#813 + gcy-8p::TU#814 + unc-122p::GFP + gcy-8p::mCherry + gcy-8p::GFP + ttx-3p::GFP]. TU#813 and TU#814 are split caspase vectors (Chalfie Lab) subcloned downstream of the gcy-8 promoter. Expression of GFP in coelomocytes and AIY neuron, and mCherry in AFD neuron when caspase activity is lost. Reference: Glauser DA, et al. Genetics. 2011 May;188(1):91-103.
JPS481 C. elegans vxEx280. Show Description
vxEx280 [sto-5p::ICE + gcy-8::ICE + myo-2p::mCherry]. Genetic ablation of AFDL, AFDR, FLPL, FLPR, BDUL, and BDUR via expression of a human cell death caspase (ICE). Reference: Russell J, et al. Proc Natl Acad Sci USA. 2014 Jun 3;111(22):8269-74.
JPS482 C. elegans tax-4(p678) III; vxEx280. Show Description
vxEx280 [sto-5p::ICE + gcy-8::ICE + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. Genetic ablation of AFD, FLP, and BDU via expression of a human cell death caspase (ICE). Reference: Russell J, et al. Proc Natl Acad Sci USA. 2014 Jun 3;111(22):8269-74.
JPS569 C. elegans che-6(e1126) IV; vxEx280. Show Description
vxEx280 [sto-5p::ICE + gcy-8::ICE + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. Genetic ablation of AFD, FLP, and BDU via expression of a human cell death caspase (ICE). Reference: Russell J, et al. Proc Natl Acad Sci USA. 2014 Jun 3;111(22):8269-74.
PY1260 C. elegans ttx-1(oy26) oyIs17 V. Show Description
oyIs17 [gcy-8p::GFP + lin-15(+)] V. Thermotaxis defective. Reduced gcy-8::GFP expression in AFD at 25C.
PY1283 C. elegans ttx-1(oy29) oyIs17 V. Show Description
oyIs17 [gcy-8p::GFP + lin-15(+)] V. Thermotaxis defective. Reduced gcy-8::GFP expression in AFD at 25C.
AX2164 C. elegans tax-2(p694) I; lin-15B&lin-15A(n765) X; dbEx725. Show Description
dbEx725 [gcy-8p::tax-2(cDNA)::SL2::GFP + gcy-32p::tax-2(cDNA)::SL2::GFP+ flp-17p::tax-2(cDNA)::SL2::GFP + flp-6p::tax-2(cDNA)::SL2::GFP + lin-15(+)]. Pick non-Muv to maintain. Array rescues tax-2 in AFD, AQR, PQR, URX, BAG, and ASE. Reference: Bretscher AJ, et al. Neuron. 2011 Mar 24;69(6):1099-113.
AX2178 C. elegans tax-2(p694) I; lin-15B&lin-15A(n765) X; dbEx726. Show Description
dbEx726 [gcy-8p::tax-2(cDNA)::SL2::GFP + lin-15(+)]. Pick non-Muv to maintain. Array rescues tax-2 in AFD. Reference: Bretscher AJ, et al. Neuron. 2011 Mar 24;69(6):1099-113.
JN2514 C. elegans peEx2514. Show Description
peEx2514 [gcy-8p::DownwardDAG2(codon-optimized) + unc-122p::mCherry]. The diacylglycerol reporter, DownwardDAG2, is expressed in AFD.
JPS271 C. elegans vxEx265. Show Description
vxEx265 [gcy-8p::ICE + myo-2p:mCherry]. Pick mCherry+ animals to maintain. Expresses human caspase (ICE) to ablate AFD neurons. Reference: Vidal-Gadea AG, et al. Elife. 2015 Jun 17;4. doi: 10.7554/eLife.07493.
JPS582 C. elegans che-6(e1126) IV; vxEx582. Show Description
vxEx582 [gcy-8p::ICE + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. Genetic ablation of AFD via expression of a human cell death caspase (ICE). Reference: Russell J, et al. Proc Natl Acad Sci USA. 2014 Jun 3;111(22):8269-74.