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Strain Species Genotype
JN1194 C. elegans gcy-14(pe1102) V. Show Description
Reference: Ortiz CO, et al., Curr Biol. 2009 Jun 23;19(12):996-1004.
OH3992 C. elegans otIs151; otEx2322. Show Description
otIs151 [ceh-36p::RFP + rol-6(su1006)]. Expresses RFP in AWCL/R and ASEL/R. otEx2322[gcy-14(prom1)::GFP + unc-122::GFP]. Expresses GFP in ASEL, and dim GFP in ASER and AWC. Expresses bright GFP in coelomocytes. Rollers. Maintain by picking GFP+.
OH4400 C. elegans lim-6(nr2073) X; otEx2322. Show Description
otEx2322 [gcy-14(prom1)::GFP + unc-122::GFP]. ASEL biased GFP expression. Expresses bright GFP in coelomocytes.
VC2398 C. elegans gcy-14(ok2236) V/nT1 [qIs51] (IV;V). Show Description
ZC412.2. Homozygous sterile deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok2236 homozygotes (sterile flaccid Unc). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GTGGATTTTTCCGGCTACAA. External right primer: AGAAAGCATTTGTGGCATCC. Internal left primer: GCAACCACGGAACCTTAAAA. Internal right primer: TTTTGTCACTGGTCTCACGC. Internal WT amplicon: 3253 bp. Deletion size: 2733 bp. Deletion left flank: TCATTCTGCTCAATAATTCCATCAAAAATT. Deletion right flank: CTCACATCGCATATGTATAACACTTTTCCG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
OH4329 C. elegans lsy-6(ot71) dpy-11(e224) V; otEx2322. Show Description
otEx2322[gcy-14(prom1)::GFP + unc-122::GFP]. Faint ASE GFP expression (2 right). Expresses bright GFP in coelomocytes. Dpy.