F47D12.1. Homozygous. Outer Left Sequence: TATGCAAATTCGTTCGAGCA. Outer Right Sequence: GAAGCACACCCGTGTTACCT. Inner Left Sequence: AGAAGCAGAAGGAGCACGAG. Inner Right Sequence: ACTGCGATTAATGGGACCTG. Inner primer WT PCR product: 2567. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

Locomotion and feeding defects. Triple mutant lacks all muscarinic signalling. Reference: Steger KA & Avery L. Genetics. 2004 Jun;167(2):633-43.

Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404.

otEx7209 [gar-2(fosmid)::GFP + inx-6(prom18)::TagRFP + pha-1(+) + OP50(genomic DNA)]. Maintain at 25C to retain array. See description of strain OH15262 for full description of otIs669 NeuroPAL (Neuronal Polychromatic Atlas of Landmarks) transgene. Reference: Yemini E, et al. Cell. 2021 Jan 7;184(1):272-288.e11. PMID: 33378642 Free pre-print available at https://www.biorxiv.org/content/10.1101/676312v2