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Strain Species Genotype
RB2067 C. elegans flp-9(ok2730) IV. Show Description
C36H8.3. Homozygous. Outer Left Sequence: AAGGGGATGGGAGAGAAGAA. Outer Right Sequence: GTAGATTTGCGGCGTTGATT. Inner Left Sequence: TGATGGCTTTCTCTTGTCCA. Inner Right Sequence: GAGCCAGATTTGGATGCACT. Inner Primer PCR Length: 1134 bp. Deletion Size: 432 bp. Deletion left flank: AAAACATTTTCTGTCGCGTTGAGGCCGTTA. Deletion right flank: CTTTAATTTTCAAAAAATTATTTCAAGAAT. Insertion Sequence: TTTTCTGTCGCGTTGAGGC. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC3745 C. elegans flp-9(gk3703[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP]) IV. Show Description
Homozygous viable. Deletion of 272 bp with Calarco/Colaiacovo selection cassette conferring myo-2::GFP and G418 resistance inserted at break. Left flanking sequence: CTCAGACTCCGCCCATACGTGTGCTCTCCA; Right flanking sequence: AGGTTCAACTTTTTCGAAAAACGAACAAAA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.
PHX3229 C. elegans flp-9(syb3229[flp-9::T2A::3xNLS::GFP]) IV. Show Description
CRISPR/Cas9 engineered tagged endogneous locus.