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FGP2 C. elegans unc-119(ed3) III; fgpIs21. Show Description
fgpIs21 [(pFGP80) pie-1p::mCherry::smo-1(GA) + unc-119(+)]. Propagate at 20-25°C. Switching to 25C prior to imaging may enhance the signal. May be used to follow unconjugated SUMO localization within the germline and embryo. When compared with strain FGP1, the difference in fluorescence signal corresponds to SUMO conjugation in FGP1. Reference: Pelisch et al. Nat Commun. 2014 Dec 5;5:5485.