| PHX5073 |
C elegans |
ceh-43(syb5073[ceh-43::SL2::GFP::H2B]) III. Show Description
GFP tag inserted into endogenous ceh-43 locus using CRISPR/Cas9 engineering. GFP expression in IL1, CEP, AIN, AIZ, ASJ, ADE, BDU, SDQ, PDE, PVQ, and possibly glia. Reference: Reilly MB, et al. bioRxiv 2022.04.29.490095; doi: https://doi.org/10.1101/2022.04.29.490095.
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| PHX5218 |
C elegans |
cest-4(syb5218[cest-4::SL2::GFP::H2B]) IV. Show Description
GFP tag inserted into endogenous cest-4 locus by CRISPR/Cas9.
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| PHX5229 |
C. elegans |
degl-2(syb5229[degl-2::SL2::gfp::H2B]) IV. Show Description
CRISPR/Cas9 engineered tagged endogenous locus.
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| PHX5413 |
C. elegans |
flp-7(syb5413[flp-7::SL2::GFP::H2B]) X. Show Description
Endogenous locus tagged with SL2::GFP::H2B using CRISPR/Cas9. Please contact Oliver Hobert prior to publishing work using this strain.
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| PHX5424 |
C. elegans |
ins-7(syb5424[ins-7::SL2::GFP::his-44]) IV. Show Description
SL2::GFP::HIS-44 tag inserted into the endogenous ins-7 locus. Reference: Sural S, et al. bioRxiv 2025.01.06.631508; doi: https://doi.org/10.1101/2025.01.06.631508.
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| PHX5452 |
C. elegans |
ins-1(syb5452[ins-1::SL2::gfp::H2B]) IV. Show Description
CRISPR/Cas9 engineered tagged endogenous locus. Reference: Cros C & Hobert O. bioRxiv 2022.04.19.488792; doi: https://doi.org/10.1101/2022.04.19.488792.
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| PHX5462 |
C. elegans |
ins-18(syb5462[ins-18::SL2::GFP::his-44]) I. Show Description
SL2::GFP::HIS-44 tag inserted into the endogenous ins-18 locus. Reference: Sural S, et al. bioRxiv 2025.01.06.631508; doi: https://doi.org/10.1101/2025.01.06.631508.
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| PHX5561 |
C. elegans |
ins-33(syb5561[ins-33::SL2::gfp::his-44]) I. Show Description
SL2::GFP::HIS-44 tag inserted into the endogenous ins-33 locus. Reference: Sural S, et al. bioRxiv 2025.01.06.631508; doi: https://doi.org/10.1101/2025.01.06.631508.
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| PHX5755 |
C. elegans |
pha-4(ot946 ot1078 syb5755[pha-4::3xGAS::GFP::3xGAS::AID*::TEV::LoxP::3xFLAG]) V. Show Description
Endogenously-tagged pha-4 locus allele modified for auxin dependent protein degradation. ot946 [pha-4::3xGAS::GFP::TEV::LoxP::3xFLAG]. ot1078 added a second loxP site to the first intron (+278). syb5755 added 3xGAS::AID* after the GFP tag. Please contact Oliver Hobert prior to publishing work using this strain.
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| PHX5923 |
C elegans |
bas-1(syb5923[bas-1::SL2::GFP::H2B]) III. Show Description
GFP tag inserted into endogenous bas-1 locus by CRISPR/Cas9.
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| PHX6078 |
C. elegans |
hlh-32(syb6078[hlh-32::GFP]) IV. Show Description
Endogenous locus tagged with GFP at C-terminus using CRISPR/Cas9. Reference: Aguilar GR, et al. PLoS Biol. 2025 Jan 6;23(1):e3002979. doi: 10.1371/journal.pbio.3002979. PMID: 39761329
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| PHX6112 |
C. elegans |
hlh-31(syb6112[hlh-31::GFP]) IV. Show Description
Endogenous locus tagged with GFP at C-terminus using CRISPR/Cas9. Reference: Aguilar GR, et al. PLoS Biol. 2025 Jan 6;23(1):e3002979. doi: 10.1371/journal.pbio.3002979. PMID: 39761329
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| PHX6129 |
C. elegans |
ieSi57 II; Y47D3A.21(syb6129[GFP::AID*:::3Xflag::3xGAS::Y47D3A.21]) III. Show Description
ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. GFP, AID*, 3xFLAG, and 3xGAS tags inserted into endogenous Y47D3A.21 locus. Reference: Sharma N, et al. bioRxiv [Preprint]. 2024 Jan 19:2024.01.16.575916. doi: 10.1101/2024.01.16.575916. PMID: 38293206.
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| PHX6236 |
C. elegans |
ins-35(syb6236[ins-35::SL2::GFP::his-44]) V. Show Description
SL2::GFP::HIS-44 tag inserted into the endogenous ins-35 locus. Reference: Sural S, et al. bioRxiv 2025.01.06.631508; doi: https://doi.org/10.1101/2025.01.06.631508.
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| PHX6265 |
C. elegans |
lgc-38(syb2346 syb6265[flp-11p::FLP D5::FLP-11 3UTR]) III. Show Description
(III:7007600). FLP D5 recombinase driver expressed from the flp-11 promoter. The flp-11p::FLP driver consistently causes recombination in RIS, as well as in several other cell types. It is more penetrant but less specific than srx-9(syb7929[srx-9::SL2::FLP D5]). Reference: Rossi L, et al. Curr Biol. 2025 Apr 20:S0960-9822(25)00355-0. doi: 10.1016/j.cub.2025.03.039. PMID: 40273913.
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| PHX6303 |
C. elegans |
hlh-17(syb6303[hlh-17::GFP]) IV. Show Description
Endogenous locus tagged with GFP at C-terminus using CRISPR/Cas9. Reference: Aguilar GR, et al. PLoS Biol. 2025 Jan 6;23(1):e3002979. doi: 10.1371/journal.pbio.3002979. PMID: 39761329
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| PHX6451 |
C. elegans |
tph-1(syb6451[tph-1::SL2::GFP::H2B]) II. Show Description
Endogenous tph-1 locus tagged by CRISPR/Cas9-engineering. Reference: Wang C, et al. Elife. 2024 Oct 18:13:RP95402. doi: 10.7554/eLife.95402. PMID: 39422452.
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| PHX6486 |
C. elegans |
cat-1(syb6486[cat-1::SL2::GFP::H2B]) X. Show Description
Endogenous cat-1 locus tagged by CRISPR/Cas9-engineering. Reference: Wang C, et al. Elife. 2024 Oct 18:13:RP95402. doi: 10.7554/eLife.95402. PMID: 39422452.
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| PHX649 |
C. elegans |
fat-6(syb649[fat-6::GFP]) IV. Show Description
GFP tag inserted into endogenous fat-6 locus using Crispr/Cas9. fat-6::GFP expression in intestine and hypodermis. No obvious phenotype, but the fat-6 locus is likely inactive in this strain. Reference: Bodhicharla R, et al. Genetics. 2018 Sep;210(1):189-201.
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| PHX6635 |
C. elegans |
hlh-17 hlh-31(syb6635) IV. Show Description
CRISPR/Cas9-engineered 5421 bp deletion entirely removing both hlh-17 and hlh-31. Reference: Aguilar GR, et al. PLoS Biol. 2025 Jan 6;23(1):e3002979. doi: 10.1371/journal.pbio.3002979. PMID: 39761329
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| PHX6730 |
C.elegans |
mab-5(syb6730[mab-5::3xFLAG::mNG::AID*)]) III. Show Description
3xFLAG, mNeonGreen, and AID* tags inserted into endogenous mab-5 locus. No phenotypes have been observed. Reference: Smith JJ, et al. Cell Rep. 2024 Mar 26;43(3):113857. doi: 10.1016/j.celrep.2024.113857. PMID: 38421866.
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| PHX679 |
C. elegans |
vglu-3(syb679[vglu-3::gfp]) III. Show Description
GFP tag inserted at C-terminus endogenous vglu-3 locus. Reference: Serrano-Saiz E, et al. Genetics. 2019 Nov 27. pii: genetics.302855.2019. PMID: 31776169
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| PHX7022 |
C. elegans |
wrt-10(syb7022[wrt-10::sfGFP]) II. Show Description
sfGFP tag inserted into endogenous wrt-10 locus using CRISPR/Cas9. Tagged inserted at C-terminus immediately preceding the stop codon. Reference: Serra ND, et al. 2024 Development. Aug 7;227(4):iyae081. doi: 10.1093/genetics/iyae081. PMID: 38739761.
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| PHX7064 |
C. elegans |
grl-2(syb7064[sfGFP::grl-2]) V. Show Description
sfGFP tag inserted into endogenous grl-2 locus using CRISPR/Cas9. Tag inserted immediately following the signal peptide. Reference: Serra ND, et al. 2024 Development. Aug 7;227(4):iyae081. doi: 10.1093/genetics/iyae081. PMID: 38739761.
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| PHX7278 |
C. elegans |
unc-46(syb7278[unc-46::SL2::GFP::H2B]) V. Show Description
Endogenous unc-46 locus tagged by CRISPR/Cas9-engineering. Reference: Wang C, et al. Elife. 2024 Oct 18:13:RP95402. doi: 10.7554/eLife.95402. PMID: 39422452.
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| PHX7352 |
C. elegans |
nlp-29(syb7352[nlp-29::SL2::GFP::H2B]) V. Show Description
Endogenous locus tagged with SL2::GFP::H2B using CRISPR/Cas9. Please contact Oliver Hobert prior to publishing work using this strain.
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| PHX7377 |
C. elegans |
nlp-7b(syb7377[nlp-7b::SL2::GFP::H2B]) X. Show Description
Endogenous nlp-7 locus (B-isoform) tagged with SL2::GFP::H2B using CRISPR/Cas9. Please contact Oliver Hobert prior to publishing work using this strain.
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| PHX7394 |
C. elegans |
nlp-36(syb7394[nlp-36::SL2::GFP::H2B]) III. Show Description
Endogenous locus tagged with SL2::GFP::H2B using CRISPR/Cas9. Please contact Oliver Hobert prior to publishing work using this strain.
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| PHX7422 |
C. elegans |
nlp-39(syb7422[nlp-39::SL2::GFP::H2B]) I. Show Description
Endogenous locus tagged with SL2::GFP::H2B using CRISPR/Cas9. Please contact Oliver Hobert prior to publishing work using this strain.
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| PHX7428 |
C. elegans |
nlp-15(syb7428[nlp-15::SL2::GFP::H2B]) I. Show Description
Endogenous locus tagged with SL2::GFP::H2B using CRISPR/Cas9. Please contact Oliver Hobert prior to publishing work using this strain.
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| PHX7515 |
C elegans |
dve-1(syb7515[dve-1::AID*::mScarlet]) X. Show Description
AID*::mScarlet tag inserted into endogenous dve-1 locus via CRISPR/Cas9 engineering. Reference: Alexander, K.D. et al. Nat Commun 2023 Nov 18;14(1):7520. PMID: 37980357.
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| PHX7537 |
C. elegans |
nlp-20(syb7537[nlp-20::SL2::GFP::H2B]) IV. Show Description
Endogenous locus tagged with SL2::GFP::H2B using CRISPR/Cas9. Please contact Oliver Hobert prior to publishing work using this strain.
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| PHX7564 |
C. elegans |
nlp-79(syb7564[nlp-79::SL2::GFP::H2B]) IV. Show Description
Endogenous locus tagged with SL2::GFP::H2B using CRISPR/Cas9. Please contact Oliver Hobert prior to publishing work using this strain.
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| PHX7566 |
C. elegans |
unc-47(syb7566[unc-47::SL2::GFP::H2B]) III. Show Description
Endogenous unc-47 locus tagged by CRISPR/Cas9-engineering. Reference: Wang C, et al. Elife. 2024 Oct 18:13:RP95402. doi: 10.7554/eLife.95402. PMID: 39422452.
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| PHX7685 |
C. elegans |
hlh-13(syb7685 [hlh-13::GFP]) X. Show Description
Endogenous locus tagged with GFP at C-terminus using CRISPR/Cas9. Reference: Aguilar GR, et al. PLoS Biol. 2025 Jan 6;23(1):e3002979. doi: 10.1371/journal.pbio.3002979. PMID: 39761329
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| PHX7688 |
C. elegans |
hlh-15(syb7688 [hlh-15::GFP]) X. Show Description
Endogenous locus tagged with GFP at C-terminus using CRISPR/Cas9. Reference: Aguilar GR, et al. PLoS Biol. 2025 Jan 6;23(1):e3002979. doi: 10.1371/journal.pbio.3002979. PMID: 39761329
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| PHX7768 |
C. elegans |
tdc-1(syb7768[GFP::linker::H2B::T2A::tdc-1]) II. Show Description
Endogenous tdc-1 locus tagged by CRISPR/Cas9-engineering. Reference: Wang C, et al. Elife. 2024 Oct 18:13:RP95402. doi: 10.7554/eLife.95402. PMID: 39422452.
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| PHX7786 |
C. elegans |
tbh-1(syb7786[tbh-1::SL2::GFP::H2B]) X. Show Description
Endogenous tbh-1 locus tagged by CRISPR/Cas9-engineering. Reference: Wang C, et al. Elife. 2024 Oct 18:13:RP95402. doi: 10.7554/eLife.95402. PMID: 39422452.
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| PHX8025 |
C. elegans |
nmr-1(syb8025[nmr-1::SL2::FLP D5]) II. Show Description
FLP D5 recombinase driver expressed from the nmr-1 locus. nmr-1::SL2::FLP causes recombination in command interneurons including AVA, AVD, AVE, RIM, AVG, PVCs. Reference: Rossi L, et al. Curr Biol. 2025 Apr 20:S0960-9822(25)00355-0. doi: 10.1016/j.cub.2025.03.039. PMID: 40273913.
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| PHX8255 |
C. elegans |
cat-2(syb8255[cat-2::SL2::GFP::H2B]) II. Show Description
Endogenous cat-2 locus tagged by CRISPR/Cas9-engineering. Reference: Wang C, et al. Elife. 2024 Oct 18:13:RP95402. doi: 10.7554/eLife.95402. PMID: 39422452.
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| PHX8406 |
C. elegans |
tol-1(syb8406[3xLinker::WrmScarlet::linker::3xFLAG]) I. Show Description
WrmScarlet and 3xFlag tags inserted into endogenous tol-1 locus. Reference: Carmona-Rosas G, et al. bioRxiv 2023.05.04.539414; doi: https://doi.org/10.1101/2023.05.04.539414.
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| PHX8408 |
C. elegans |
lat-1(syb8408[lat-1AAAAA::EGFP::linker::3xFLAG::AAAAA]) II. Show Description
Internal eGFP and FLAG tags with poly-A linkers inserted into endogenous lat-1 locus before 651 aa. Reference: Carmona-Rosas G, et al. bioRxiv 2023.05.04.539414; doi: https://doi.org/10.1101/2023.05.04.539414.
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| PHX8612 |
C. elegans |
bcat-1(syb8612[bcat-1::SL2::GFP::H2B]) X. Show Description
Endogenous locus tagged with SL2::GFP::H2B at C-terminus using CRISPR/Cas9. Reference: Aguilar GR, et al. PLoS Biol. 2025 Jan 6;23(1):e3002979. doi: 10.1371/journal.pbio.3002979. PMID: 39761329
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| PHX8870 |
C. elegans |
oct-1(syb8870[oct-1::SL2::GFP::H2B]) I. Show Description
Endogenous oct-1 locus tagged by CRISPR/Cas9-engineering. Reference: Wang C, et al. Elife. 2024 Oct 18:13:RP95402. doi: 10.7554/eLife.95402. PMID: 39422452.
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| PHX8955 |
C. elegans |
lat-1(syb8955[lat-1 F69A] *syb8408) II. Show Description
Engineered F69A mutation in endogenously-tagged lat-1 locus. Slightly reduced brood size. syb8408 is internal eGFP and FLAG tags with poly-A linkers inserted into endogenous lat-1 locus before 651 aa. Reference: Carmona-Rosas G, et al. bioRxiv 2023.05.04.539414; doi: https://doi.org/10.1101/2023.05.04.539414.
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| PHX9007 |
C. elegans |
unc-4(syb9007[unc-4::SL2::FLP D5]) II. Show Description
FLP D5 recombinase driver expressed from the unc-4 locus. unc-4::SL2::FLP causes recombination specifically in Dorsal A-type motor neurons, SABs neurons and I5. Reference: Rossi L, et al. Curr Biol. 2025 Apr 20:S0960-9822(25)00355-0. doi: 10.1016/j.cub.2025.03.039. PMID: 40273913.
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| PJ1043 |
C. elegans |
tpa-1(k501) IV; ccIs55 V. Show Description
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Superficially wild-type. Resistant to PMA.
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| PJ1046 |
C. elegans |
cha-1(p1182) IV; ccIs55 V. Show Description
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Unc lethal at 25C. Difficult to score <25C.
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| PJ1124 |
C. elegans |
mek-2(ku114) I; clr-1(e1745) II; ccIs55 V. Show Description
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Egl. Bags at high frequency. Semi-Clr.
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| PJ1153 |
C. elegans |
clr-1(e1745) II; let-756(s2613) unc-32(e189) III; ccIs55 V. Show Description
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Homozygous viable and fertile, but slow-growing, transparent, and small (but not scrawny). Coiler Unc. clr-1 is temperature-sensitive.
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