BA1073 |
C. elegans |
dyf-5(hc183) I. Show Description
Deletion allele of M04C9.5 (mak-kinase). No visible phenotype in hermaphrodites. Primers HES 186/187 outer and HES 188/189 inner.
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BA1083 |
C. elegans |
F43G6.6(hc184) II. Show Description
Deletion allele of F43G6.6. No obvious phenotype in hermaphrodites. HES primers 162/163 outer and 164/165 inner.
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BA1084 |
C. elegans |
F09C12.2(hc185) II. Show Description
Deletion allele of F09C12.2. No obvious phenotype in hermaphrodites. HES primers 122/123 outer and HES 124/125 inner.
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BA1090 |
C. elegans |
cav-2(hc191) V. Show Description
No visible phenotype in hermaphrodites. Deletion allele of C56A3.7.
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BA1093 |
C. elegans |
snf-10(hc194) V. Show Description
Y32F6A.2 No visible phenotype in hermaphrodites. Primers: outer HES 22/228 and inner HES 229/230.
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BA14 |
C. elegans |
fer-14(hc14) X. Show Description
Temperature sensitive. 8% of total oocytes produced are fertilized and give rise to larvae at 16C. Almost completely sterile at 25C.
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BA15 |
C. elegans |
rrf-3(hc15) II. Show Description
Temperature sensitive. Maintain at 15C.
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BA606 |
C. elegans |
spe-6(hc49) unc-25(e156) III; eDp6 (III;f). Show Description
Animals with the Duplication are WT. Animals without the Duplication are Unc and Sterile.
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BA609 |
C. elegans |
spe-6(hc49) vab-7(e1562) III; eDp6 (III;f). Show Description
Animals with the Duplications are WT. Animals which have lost the Duplication are Sterile and have an abnormal tail.
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BA671 |
C. elegans |
spe-9(hc88) I. Show Description
Self-sterile at 25C. Maintain at 15C.
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BA676 |
C. elegans |
spe-6(hc92) unc-32(e189) III; eDp6 (III;f). Show Description
Unc. Animals which have lost the duplications are Sterile and Unc.
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BA717 |
C. elegans |
spe-11(hc90) I; sDp2 (I;f). Show Description
Animals carrying the duplication WT. Animals which have lost the Dup are sterile. Maintain by picking fertile animals and checking for segregation of fertile and sterile progeny.
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BA759 |
C. elegans |
hcDf1 IV; eEx25. Show Description
eEx25 [C13G4(cosmid) + XDM23(phage)]. Animals with eEx25 are WT. Pick wild-type to maintain. Animals which have lost eEx25 are Twitchers and Sterile (occasionally produce young). This cosmid lacks the 3' end of the unc-22 gene while XDM23 lacks the 5' end of unc-22, but following injection an extrachromosomal array was formed that included at least one functional unc-22 gene.
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BA763 |
C. elegans |
spe-5(hc93) I; sDp2 (I;f). Show Description
Animals with the Duplications are WT. Animals which have lost the Duplication are Sterile.
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BA783 |
C. elegans |
spe-12(hc76) I. Show Description
Hermaphrodites produce nonfunctional sperm and are fertilization defective. Self-fertility is <1% at 16C or 25C. Males are fertile.
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BA785 |
C. elegans |
spe-8(hc40) I. Show Description
Hermaphrodites produce nonfunctional, nonmotile sperm with uniformly aberrant pseudopods. Male sperm normal. Self-fertility is <1% at 16C or 25C. Maintain by mating.
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BA900 |
C. elegans |
spe-27(it110) IV. Show Description
Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps, males fertile. Spermatids activate "normally" in TEA. In pronase they produce spikes but never form pseudopods. Similar to spe-12 and spe-8.
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BA901 |
C. elegans |
spe-27(it110) dpy-20(e1282) IV. Show Description
Temperature sensitive Dpy. Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps; males fertile. Spermatids arrest with spikes in pronase; form normal pseudopods in TEA.
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BA947 |
C. elegans |
spe-27(hc161) IV. Show Description
Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps; males fertile.
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BA962 |
C. elegans |
spe-29(it127) IV. Show Description
Hermaphrodites are sterile; Males are fertile. Hermaphrodites lay oocytes (produce a few fertile eggs and many oocytes). Produce viable progeny when mated to males. Hermaphrodites produce 10X more self progeny at 20C (2.5/herm) than at either 16C or 25C.
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BA968 |
C. elegans |
spe-29(it127) unc-24(e138) IV. Show Description
Unc. Leaky sterile at 20C; tighter at 16C and 25C. Easier to propagate as if male-female strain.
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BA969 |
C. elegans |
spe-6(hc163) III; spe-27(it132) dpy-20(e1282) IV. Show Description
Dpy. spe-6(hc163) is a recessive suppressor of spe-27(it132). spe-6(hc163) also suppresses spe-8, spe-12, spe-29 and other spe-27 alleles. Causes precocious spermatid activation. Fertile between 15-25C.
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BA970 |
C. elegans |
spe-6(hc163) III; spe-27(it132) unc-22(e66) IV. Show Description
Twitcher Unc. spe-6(hc163) is a recessive suppressor of spe-27(it132). spe-6(hc163) also suppresses spe-8, spe-12, spe-29 and other spe-27 alleles. Causes precocious spermatid activation. Fertile between 15-25C.
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BA975 |
C. elegans |
spe-6(hc163) III; spe-29(it127) dpy-20(e1282) IV. Show Description
spe-6(hc163) suppresses the self-sterility of spe-29 in this strain. Self-sterile at 25C.
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BA979 |
C. elegans |
dpy-5(e61) spe-12(hc76) I; spe-6(hc163) III. Show Description
spe-6(hc163) suppresses hermaphrodite self-sterility of hc76. Dpy.
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BA984 |
C. elegans |
spe-6(hc163) dpy-18(e364) III. Show Description
Dpy. spe-6(hc163) suppresses self-sterility of spe-8, spe-12, spe-27, and spe-29 mutants. Causes precocious spermatide activation. Fertile between 15-25C.
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BA989 |
C. elegans |
spe-6(hc163) III; spe-27(it132) IV. Show Description
spe-6(hc163) suppresses the ts self-sterile phenotype of spe-27(it132). Also suppresses spe-8, spe-12, and spe-29. Causes precocious spermatid activation. Lays eggs and oocytes. Males are weakly fertile. Fertile between 15-25C.
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BB19 |
C. elegans |
adr-1(tm668) I. Show Description
Reduced lifespan, chemotaxis defective, co-suppression of transgenes in somatic cells. Maintain under normal conditions. Reference: Hundley HA, et al. RNA. 2008 Oct;14(10):2050-60.
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BB21 |
C. elegans |
adr-1(tm668) I; adr-2(ok735) III. Show Description
Reduced lifespan, chemotaxis defective, co-suppression of transgenes in somatic cells. Maintain under normal conditions. Reference: Hundley HA, et al. RNA. 2008 Oct;14(10):2050-60.
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BB259 |
C. elegans |
adr-1(uu49) I; adr-2(uu28) III; ggIs1 IV. Show Description
ggIs1 [nrde-3p::3xFlag::GFP::nrde-3 ORF + unc-119(+)] IV. Nuclear GFP::NRDE-3 signal. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282.
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BB261 |
C. elegans |
adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) III. Show Description
Enhanced RNAi. Lacks RNA editing. Vulval bursting. Low brood size. Triple mutants display bursting and low brood size phenotypes not observed in adr-1;adr-2 or rrf-3 parental strains. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282.
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BB270 |
C. elegans |
adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) III; rde-1(uu51) V. Show Description
RNAi deficient. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282.
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BB272 |
C. elegans |
adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) rde-4(uu53) III. Show Description
RNAi deficient. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282.
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BB278 |
C. elegans |
adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) III; ggIs1 IV. Show Description
ggIs1 [nrde-3p::3xFlag::GFP::nrde-3 ORF + unc-119(+)] IV. Nuclear GFP::NRDE-3 signal. Enhanced RNAi. Lacks RNA editing. Vulval bursting. Low brood size. Triple mutants display bursting and low brood size phenotypes not observed in adr-1;adr-2 or rrf-3 parental strains. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282.
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BB283 |
C. elegans |
adr-1(uu49) I; adr-2(uu28) III; ergo-1(uu68) V. Show Description
Enhanced RNAi. Lacks RNA editing. Vulval bursting. Low brood size. Triple mutants display bursting and low brood size phenotypes not observed in adr-1;adr-2 or rrf-3 parental strains. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282.
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BC14133 |
C. elegans |
dpy-5(e907) I; sEx14133. Show Description
sEx14133[rCesB0350.2f::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
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BC1519 |
C. elegans |
dpy-18(e364)/eT1 III; sDf31/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. Original isolation: BO strain BC1261 unc-22(s727) hermaphrodite heat shocked for 1 hour at 34C. Crossed to N2 strain BC1270 unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Picked individual WT hermaphrodites. Screened for the absence of gravid Unc-22. Retained strain as BC1379. Balanced over eT1: BC1379 unc-22(s727)[BO]/nT1[N2] IV; sDf31[BO]/nT1[N2] hermaphrodite crossed to BC 1265 dpy-18(e364)/eT1 III; unc-46(e177)/eT1 V. Pick WT hermaphrodites that twitch in 1% nicotine. Retained one strain that segregated Unc-36 as BC1428. Pseudolinked sDf31 to dpy-18: BC1428 +/eT1 III; sDf31[BO]/eT1 V crossed to BC 1265 dpy-18/eT1 III; unc-46/eT1 V male. Picked WT hermaphrodite F1. From strains segregating Unc-36 but no Dpy or Unc-46 or DpyUnc-46, cross WT hermaphrodites to BC1265 males. From strains producing Dpy, crossed individual WT males to BC70 eT1;eT1. From each cross, crossed WT X WT. Kept one strain producing on Dpy or DpyUncs as S-H716 male. Picked one hermaphrodite to start BC1519. Comments: 1) The LGV region balanced by eT1 should be all BO. 2) Probably the rest of the genome still has a considerable amount from BO since it was crossed to N2 only 4 times. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
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BC159 |
C. elegans |
dpy-5(e61) unc-13(e51) I; sDp1 (I;f). Show Description
Animals with the duplication are WT. Animals which have lost the duplication are DpyUnc. Segregates 2% males. The males are sterile. The duplication is homozygous lethal.
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BC1972 |
C. briggsae |
Cbr-unc(s1270). Show Description
C. briggsae strain. Twitcher. Hets twitch only in prescence of nicotine.
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BC2010 |
C. elegans |
let-60(s1124) unc-22(s7) unc-31(e169) IV/nT1 (IV;V). Show Description
Heterozygotes are WT and segregate WT, Vul and UncLethals. Strong allele of let-60. Unc Twitchers arrest as rod-like early larvae; rare escapers die as Vul adults.
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BC2383 |
C. elegans |
dpy-18(e364)/eT1 III; unc-46(e177) let-469(s1582)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal as sterile adult. Maintain by picking WT. [7/94: No longer throwing eT1 Unc-36's. May have picked up a lethal mutation on one of the eT1 chromosomes. Also, appears to be lethal in mid-late larval stage rather than as adult.]
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BC3134 |
C. elegans |
srl-2(s2507) dpy-18(e364) III; unc-46(e177) rol-3(s1040) V. Show Description
srl-2 is a recessive suppressor of the s1040 temperature sensitive lethal phenotype. srl-2 has no effect on the Roller phenotype of s1040. (At 20C, s1040 homozygotes arrest development at mid-larval stage. At 15C, s1040 homozygotes are viable weak left-handed rollers.) Maintain at 20C to select for retention of suppressor. Animals at 20C will be DpyUncRol.
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BC4011 |
C. elegans |
srl-1(s2500) II; dpy-18(e364) III; unc-46(e177) rol-3(s1040) V. Show Description
srl-1 is a recessive suppressor of the s1040 temperature sensitive lethal phenotype. srl-1 has no effect on the Roller phenotype of s1040. (At 20C, s1040 homozygotes arrest development at mid-larval stage. At 15C, s1040 homozygotes are viable weak left-handed rollers.) Maintain at 20C to select for retention of suppressor. Animals at 20C will be DpyUncRol.
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BC4142 |
C. elegans |
dpy-17(e164) let-769(s2432) unc-32(e189) III; sDp3 (III;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in mid to late larval development.
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BC4143 |
C. elegans |
dpy-17(e164) mup-4(s2433) unc-32(e189) III; sDp3 (III;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in early larval development. s2433 previously assigned to let-701.
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BC4144 |
C. elegans |
dpy-17(e164) let-723(s2434) unc-32(e189) III; sDp3 (III;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and Sterile as adults.
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BC4145 |
C. elegans |
let-738(s2435) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication arrest as embryos.
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BC4148 |
C. elegans |
dpy-17(e164) mel-31(s2438) unc-32(e189) III; sDp3 (III;f). Show Description
Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and give only dead eggs.
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BC4149 |
C. elegans |
dpy-17(e164) let-712(s2439) unc-32(e189) III; sDp3 (III;f). Show Description
Maintain by picking Uncs. DpyUncs arrest as early larvae.
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BC4151 |
C. elegans |
dpy-17(e164) let-972(s2441) unc-32(e189) III; sDp3 (III;f). Show Description
Maintain by picking Uncs. DpyUncs arrest at early larvae. s2441 previously assigned to let-703.
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