Temperature sensitive sterile. Maintain at 20C. Fertility is <10% of WT at 25C.

Temperature sensitive embryonic lethal. Maintain at 15 C. 6% hatching rate at 25 C. Reference: Johnston et al. (2010) Curr Biol.

B0244.8. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok1459 homozygotes (small, sterile with vulval blip). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

asIs2 [pie-1p::GFP::egg-1 + unc-119(+)]. Oocyte membranes are GFP+.

asIs3 [egg-1::GFP + unc-119(+)]. Oocyte membranes are green.