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Strain Species Genotype
JK967 C. elegans ubr-5(q298) I; unc-32(e189) glp-1(q231) III. Show Description
Unc. Fertile with viable progeny at or below 20C. Glp-1 sterile at higher temperatures. No obvious visible phenotype associated with sog-1. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK968 C. elegans unc-32(e189) glp-1(q231) III; sog-6(q306) IV. Show Description
Unc. Fertile with viable progeny at or below 20C. Glp-1 sterile at higher temperatures. No obvious visible phenotype associated with sog-6. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
LB128 C. elegans atp-2(ua2) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III. Show Description
Heterozygotes are WT and segregate WT, Dpy(ts) Steriles and Uncs which arrest in the L3 larval stage. ua2 is a deletion of the first 2 exons of atp-2. atp-2 gene encodes for active site subunit of Complex V of mitochondrial respiratory chain, the ATP synthase.
MH37 C. elegans mpk-1(ku1) unc-32(e189) III. Show Description
Unc. ku1 pka sur-1(ku1).
ML1214 C. elegans pros-1(mc41)/dpy-17(e164) unc-32(e189) III. Show Description
Heterozygotes are WT and segregate WT, Dpy Uncs, and dead L2 larvae that are translucent and often found away from the bacterial lawn (can be difficult to spot on the lawn). [NOTE: mc41 was initially described as an allele in rdy-3, but the affected gene has since been identified as pros-1]
MT1373 C. elegans lin-13(n387)/unc-32(e189) III; him-5(e1467) V. Show Description
Hets are WT. At 25C hets segregate WT, Unc, Sterile Muv and males. Maintain by picking WT. ts maternal effect.
MT1422 C. elegans paqr-2(n573) unc-32(e189) III. Show Description
Egl. Unc.
MT1799 C. elegans lin-36(n766) unc-32(e189) III. Show Description
Unc.
MT1908 C. elegans nDf21/dpy-19(e1259) unc-32(e189) III. Show Description
Heterozygotes are Dpy and segregate Dpy, DpyUncs and dead eggs.
MT1909 C. elegans nDf22/dpy-19(e1259) unc-32(e189) III. Show Description
Heterozygotes are Dpy and segregate Dpy, DpyUnc and dead eggs. e1259 has a ts maternal effect.
MT20108 C. elegans dpy-17(e164) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] nIs281 III. Show Description
nIs281 [myo-2::RFP] integrated near qC1. Recombination between nIs281 and qC1 has been reported. Fails to complemement all markers on qC1. Heterozygotes are WT. Segregates Dpy Sterile and Dpy Unc.
MT20109 C. elegans dpy-17(e164) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] nIs189 III. Show Description
nIs189 [myo-2::GFP] integrated in or near qC1. No recombination seen between nIs189 and qC1. Fails to complement all markers on qC1. Heterozygotes are WT GFP+. Segregates GFP+ Dpy Sterile and non-GFP Dpy Unc.
MT20113 C. elegans unc-32(e189) dpy-18(e499)/eT1 III; +/eT1 nIs267 V. Show Description
nIs267 [myo-2::GFP] integrated in or near eT1. Heterozygotes are wild-type and segregate WT, Dpy Unc, and Unc. Maintain by picking wild-type; check for presence of Unc progeny.
MT2343 C. elegans dpy-19(e1259) lin-12(n137)/unc-32(e189) lin-12(n137n720) III. Show Description
Heterozygotes are Muv and Egl (n137 is semi-dominant) and segregate DpyMuv and Unc lethals (most arrest as L1-L3, the few survivors are sterile, scrawny, Unc and have a large ventral blip at hte position of the vulva). Maintain by picking non-Dpy non-Unc.
MT2351 C. elegans lin-10(e1439) I; dpy-19(e1259) lin-12(n137)/unc-32(e189) lin-12(n137n720) III. Show Description
Heterozygotes are Muv. Segregates DpyMuv and Unc.
MT3022 C. elegans nDf20/sma-2(e502) unc-32(e189) III. Show Description
Heterozygotes are WT and segregate WT, SmaUnc and dead eggs. Maintain by picking WT.
MT3025 C. elegans let-23(n1045) II; lin-13(n387) unc-36(e251)/unc-32(e189) III. Show Description
Heterozygotes are Vulvaless and segregate more Vul, Vul Unc-36 and Vul Unc-32. n1045 is cold sensitive-strain gives some arrested larvae (L2) which look like little sticks.
MT3751 C. elegans dpy-5(e61) I; rol-6(e187) II; unc-32(e189) III. Show Description
MT4009 C. elegans lin-39(n1760)/dpy-17(e164) unc-32(e189) III. Show Description
Heterozygotes are WT and segregate WT, DpyUnc and Vul. Pick WT to maintain.
MT4500 C. elegans lin-39(n1880)/dpy-17(e164) unc-32(e189) III. Show Description
Heterozygotes are WT and segregate WT, DpyUnc and Vul. Pick WT to maintain.
MT465 C. elegans dpy-5(e61) I; bli-2(e768) II; unc-32(e189) III. Show Description
Mapping strain. DpyUnc.
MT4947 C. elegans ced-1(e1735) I; unc-32(e189) ced-7(n1892) III. Show Description
Cell corpses persist. Unc. n1892 is the strongest allele of ced-7.
MT4979 C. elegans lon-1(e185) ced-6(n1813) unc-32(e189) ced-7(n1892) III. Show Description
ced-6(n1813): dead cells persist, maternal rescue of embryonic. Unc. Long.
MT4997 C. elegans unc-32(e189) ced-7(n1892) III. Show Description
Unc. Persistent cell corpses-Maternal effect.
MT5000 C. elegans unc-32(e189) ced-7(n1892) III; ced-10(n1993) IV. Show Description
Unc.
MT688 C. elegans lin-12(n137)/unc-32(e189) III; him-5(e1467) V. Show Description
Heterozygotes are Muv and segregate Muv, Unc and males. Maintain by picking Muv which throw Uncs.
MT706 C. elegans lin-13(n388)/unc-32(e189) III. Show Description
Heterozygotes are WT and segregate WT, Uncs and Sterile Muvs. Maintain by picking WT.
NG2618 C. elegans yDf10 unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III. Show Description
Heterozygotes are WT and segregate WT, Dpy Steriles and dead eggs. Derived from strain TY1353. Grows fairly slowly but seems more stable than TY1353, which gives lots of steriles.
OH2638 C. elegans dpy-17(e164) let-756(s2887) unc-32(e189) III; oyIs14 V; otEx1467. Show Description
oyIs14 [sra-6::GFP + lin-15(+)]. otEx1467 [let-756(+) + ceh-22p::GFP]. Animals carrying otEx1467 are DpyUnc. Animals which have lost the otEx1467 array are DpyUncLet.
OH2639 C. elegans dpy-17(e164) let-756(s2887) unc-32(e189) III; oyIs14 V; otEx1468. Show Description
oyIs14 [sra-6::GFP + lin-15(+)]. otEx1468 [let-756(+) + ceh-22p::GFP]. Animals carrying otEx1468 are DpyUnc. Animals which have lost the otEx1468 array are DpyUncLet.
PJ1153 C. elegans clr-1(e1745) II; let-756(s2613) unc-32(e189) III; ccIs55 V. Show Description
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Homozygous viable and fertile, but slow-growing, transparent, and small (but not scrawny). Coiler Unc. clr-1 is temperature-sensitive.
PJ1155 C. elegans let-756(s2613) unc-32(e189) III; ccIs55 V; egl-17(n1377) X. Show Description
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Egg laying defective. Moderate to severe bloating. >50% make bags of worms.
PJ1158 C. elegans clr-1(e1745) II; let-756(s2613) unc-32(e189) III; ccIs55 V; egl-17(n1377) X. Show Description
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Egl: moderate to severe bloating. Clr-1 is ts. Homozygous viable and fertile, but slow-growing, transparent, and small (but not scrawny). Coiler Unc.
PS2943 C. elegans hmg-1.2(sy549) unc-32(e189) III; syIs20 him-5(e1490) V. Show Description
syIs20 [gpa-1::lacZ + dpy-20(+)] V. Unc. Him. p-Vul. Abnormal secondary vulva lineage defect in 10% of animals. Reduced brood size as well as other pleiotropic defects. Males have ray defect (cannot mate) and SPD sheath to neuron transformation, which can be visualized by lacZ staining in syIs20 [gpa-1::GFP] background: 1 cell per spicule stains in WT (the SPD neuron) whereas in hmg-1.2(sy549), 2 cells per spicule stain. sy549 previously called son-1. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
PS3996 C. elegans lin-10(e1439) I; dpy-19(e1259) lin-12(n137)/unc-32(e189) lin-12(n137n720) III; syIs57. Show Description
syIs57 [chd-3::CFP]. Anchor cell present in approx. 1% d/o animals.
RL67 C. elegans lon-1(e185) let-711(it150) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III. Show Description
Heterozygotes are WT and segregate WT, Dpy Steriles, and Lon Uncs which are temperature sensitive maternal effect lethals. Embryos from homozygyous it150 hermaphrodites have spindle orientation defects at second and third cleave at 25C.
RW1522 C. elegans pat-2(st538) unc-32(e189) III; stEx10. Show Description
Animals with the extrachromosomal array are Unc Rollers. Animals which have lost the array are Pat. Strain can be propagated by chunking. The exact construct used to create stEx10 is unknown; it is thought to carry a pat-2 rescuing construct (most likely a cosmid) with a Rol marker.
SP17 C. elegans unc-32(e189) dpy-1(e1) III. Show Description
DpyUnc.
SP462 C. elegans dpy-1(e1) unc-36(e251) III; mnDp37[unc-32(e189)] (III;f). Show Description
Animals with the duplication are WT. Animals which have lost the duplication are DpyUnc-36.
SP471 C. elegans dpy-17(e164) unc-32(e189) III. Show Description
ST20 C. elegans ncIs2 II; mup-4(nc20)/sma-3(e491) unc-32(e189) III. Show Description
ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, SmaUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development or as embryos. Not well balanced. Neurons visualized with NcIs2.
ST21 C. elegans ncIs2 II; mup-4(nc21)/dpy-17(e164) unc-32(e189) III. Show Description
ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, DpyUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development or as embryos. Not well balanced. Neurons visualized with NcIs2.
ST22 C. elegans ncIs2 II; mup-4(nc22)/dpy-17(e164) unc-32(e189) III. Show Description
ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, DpyUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development or as embryos. Not well balanced. Neurons visualized with NcIs2.
ST35 C. elegans ncIs2 II; mup-4(nc35)/dpy-17(e164) unc-32(e189) III. Show Description
ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, DpyUncs, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest as embyros or in larval development. Not well balanced. Neurons visualized with ncIs2.
SX340 C. elegans unc-32(e189) mut-7(pk204) pha-1(e2123) III; ccEx7271. Show Description
ccEx7271 [let-858::GFP + pha-1(+)]. This strain expresses nuclear-localized GFP in all somatic nuclei, but reduced or no GFP in germ cells. If maintained at 20C, pha-1(ts) genotype will select for transgenic animals. Germ cell expression can be observed when maintained at 25C. Germline transgene silencing is abnormal.
TY1353 C. elegans yDf10 unc-32(e189)/unc-93(e1500) dpy-17(e164) III. Show Description
Heterozygotes are Unc-93 and segregate more Unc-93, yDf10 homozygotes (dead eggs) and Unc-93 Dpy-17 homozygotes (young dpy-17 larvae are easily recognizable as abnormal spindle-shaped things). Difficult to maintain and use. yDf10 apparently causes semi-sterility (a second strain constructed by the Mark Edgley at the CGC, yDf10/qC1, exhibits same sterility), and unc-93 is Egl and difficult to mate into. Some Df homozygotes are laid, but most remain inside the mother.
TY415 C. elegans unc-32(e189) dpy-28(s939) III/eT1 III; +/eT1 V. Show Description
WT strain which segregates WT, Unc-36, DpyUnc and dead eggs. DpyUncs give 6-10% viable progeny at 20C and less than 1% at 15C. Maintain by picking WT.
TY562 C. elegans +/szT1 [lon-2(e678)] I; unc-32(e189) III; yDf3/szT1 X. Show Description
Unc. Throws Unc, UncLon males and dead eggs.
TY567 C. elegans +/szT1 [lon-2(e678)] I; unc-32(e189) III; yDf2/szT1 X. Show Description
Unc. Throws Unc, UncLon males and dead eggs.
TY578 C. elegans +/szT1 [lon-2(e678)] I; unc-32(e189) III; yDf5/szT1 X. Show Description
Unc. Throws Unc, UncLon males and dead eggs.