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Strain Species Genotype
ANA65 C. elegans adeIs1 II; unc-119(ed3) III. Show Description
adeIs1 [mex-5::spd-1::GFP + unc-119(+)] II. The transgene has been inserted on chromosome II, using the MosSCI technique. This strains expresses the SPD-1 protein fused to GFP in the germline (both males and females) and in embryos. SPD-1 is nucleolar in interphase and labels the central spindle in mitosis and meiosis, later accumulating at the midbody. Reference: Nahaboo W, et al. Mol Biol Cell. 2015 Jun 1;26(11):2020-9. doi: 10.1091/mbc.E14-12-1577.
AS338 C. elegans dpy-6(e14) gly-13(ok712) X. Show Description
CB14 C. elegans dpy-6(e14) X. Show Description
Dpy.
CB3816 C. elegans tra-3(e1107) IV; unc-58(e665) sup-21(e1957) dpy-6(e14)/+ X. Show Description
Heterozygotes are Unc (shaker; don't move well) and segregate more Shaker Unc, WT and DpyUnc (short and fairly paralyzed). The WT give only males. e1957 previously called sup-21.
CF816 C. elegans him-5(e14??) V; ref-2(mu218) X. Show Description
In males, P7.p and P8.p fail to fuse with hyp7 at the end of L1. Dominant. him-5 allele is either e1467 or e1490.
CH121 C. elegans dgn-1(cg121)/dpy-6(e14) unc-115(mn481) X. Show Description
Heterozygotes are WT and segregate WT, DpyUncs, and Ste (and Gon) cg121 homozygotes.
DA711 C. elegans unc-10(e102) dpy-6(e14) X. Show Description
DpyUnc.
DR1290 C. elegans dpy-6(e14) unc-3(e151) X. Show Description
Medium sized Dpy which curls its tail.
EM122 C. elegans stDp2 (X;II)/+ II; him-5(e1490) V; unc-18(e81) dpy-6(e14) X. Show Description
Strain throws WT, DpyUncs and males (and some Lon-don't know where these come from??). Maintain by picking WT. non-Unc non-Dpy males mate at low frequency and will transmit stDp2.
EU307 C. elegans +/szT1 [lon-2(e678)] I; mom-1(or46) dpy-6(e14)/szT1 X. Show Description
Heterozygotes are WT and segregate WT, Lon males, lots of dead eggs, and Dpys. The Dpys should give all dead embryos at all temperatures. Approximately 60% of these dead embryos make excess mesoderm at the expense of endoderm, due to an E to MS fate transformation. Embryonic lethality is 100% penetrant for or46.
EU308 C. elegans +/szT1 [lon-2(e678)] I; mom-1(or10) dpy-6(e14)/szT1 X. Show Description
Heterozygotes are WT and segregate WT, Lon males, lots of dead eggs, and Dpys. The Dpys should give all dead embryos at all temperatures. Approximately 60% of these dead embryos make excess mesoderm at the expense of endoderm, due to an E to MS fate transformation. Embryonic lethality is 100% penetrant for or10.
MT3460 C. elegans +/szT1 I; dpy-6(e14) egl-15(n1454)/szT1 [lon-2(e678)] X. Show Description
WT strain which segregates WT, Dpy L1 lethals, Lon males and dead eggs. Class II egl-15 mutation.
MT5222 C. elegans sem-5(n2030)/unc-10(e102) xol-1(y9) dpy-6(e14) X. Show Description
Heterozygotes are WT and segregate WT, Vuls (bags) and DpyUncs. n2030: Vul; impenetrant Mel (rod-like larval-lethal).
SP1981 C. elegans unc-115(mn481) dpy-6(e14) X; stDp2 (X;II)/+. Show Description
Wt strain throws WT and DpyUncs.
SP64 C. elegans unc-6(e78) dpy-6(e14) X. Show Description
Dpy. Unc.
SP928 C. elegans dpy-6(e14) unc-9(e101) X. Show Description
DpyUnc.
TY1702 C. elegans unc-42(e270) yDf12 V/nT1 [unc-?(n754) let-?] (IV;V); dpy-6(e14) X. Show Description
Heterozygotes are DpyUnc and segregate DpyUnc and dead eggs.
TY2782 C. elegans fox-1(y303) dpy-6(e14) X. Show Description
VT191 C. elegans +/szT1 [lon-2(e678)] I; dpy-6(e14) lin-14(n536) maDf1/szT1 X. Show Description
VT192 C. elegans +/szT1 [lon-2(e678)] I; dpy-6(e14) lin-14(n536) maDf2/szT1 X. Show Description
VT333 C. elegans +/szT1 [lon-2(e678)] I; dpy-17(e164) III; dpy-6(e14) lin-14(n536) maDf2/szT1 X. Show Description
Heterozygotes are Dpy and segregate Dpy, males and dead eggs.
AD271 C. elegans spe-38(eb44) I; him-5(e1490) V; asEx78. Show Description
asEx78 [spe-38p::spe-38(cDNA)::spe-38 3'UTR + myo-3p::GFP]. Pick GFP+ to maintain. GFP+ worms are fertile; animals that have lost the array are sterile.
AD281 C. elegans spe-45(as38) IV; him-5(e1490) V. Show Description
Him. Temperature-sensitive sterile. Small brood size even at permissive temperatures; pick fertile animals and maintain at 15C. Worms lacking spe-45 function produce morphologically normal and motile sperm that cannot fuse with oocytes despite direct contact in the reproductive tract. spe-45 hermaphrodites and males are subfertile at 16C and sterile at 25C. Reference: Singaravelu G, et al. Current Biology 2015. http://dx.doi.org/10.1016/j.cub.2015.10.055
AD294 C. elegans cylc-2(mon2[cylc-2::mNG::3xFLAG) I; him-5(e1490) V. Show Description
Endogenous cycl-2 locus tagged with mNeonGreen (mNG). Green fluorescence in sperm. Him. Reference: Krauchunas AR, et al. (2020). C. elegans CYLC-2 localizes to sperm. microPublication Biology. 10.17912/micropub.biology.000314.
AG152 C. elegans unc-85(e1414) bli-2(e768) dpy-10(e128) II. Show Description
Unc and Dpy. Not Blistered: dpy-10 suppresses the appearance of the blisters.
AG226 C. elegans rol-6(e187) unc-4 (e120)/mnC1 [dpy-10(e128) unc-52(e444) nIs190 let-?] II; him-8(e1489) IV. Show Description
nIs190 [myo-2::GFP]. Him. Heterozygotes are wild-type GFP+ and segregate WT GFP+ heterozygotes, Rol Uncs, dead embryos, and males. nIs190 [myo-2::GFP] integrated in or near mnC1. Approx 0.5% recombination seen between nIs190 and mnC1. Fails to complemement all markers on mnC1.
AV285 C. elegans swm-1(me66) him-5(e1490) V. Show Description
Sperm activation in virgin males. Poor sperm transfer.
AV322 C. elegans swm-1(me87) him-5(e1490) V. Show Description
Sperm activation in virgin males. Very poor sperm transfer.
BA1044 C. elegans spe-27(it132) spe-29(it127) IV; him-5(e1490) V. Show Description
Non-conditional hermaphrodite sterile. Males fertile. Self-progeny produced after mating.
BA1051 C. elegans spe-12(hc76) I; spe-29(it127) IV; him-5(e1490) V. Show Description
Self-sterile hermaphrodites; fertile males. Mated hermaphrodites produce self and outcross progeny. Self progeny are 33% males.
BA524 C. elegans fer-1(hc1) I; him-5(e1490) V. Show Description
Temperature sensitive. Maintain at 15C. Throws males.
BA547 C. elegans fer-2(hc2) III; him-5(e1490) V. Show Description
Fertile at 16c and 20C. Sterile at 25C-Produces some defective embryos and 0-5 progeny. Throws males.
BA562 C. elegans fer-4(hc4) him-5(e1490) V. Show Description
Temperature sensitive. Maintain at 15C. Produces about 30% males.
BA782 C. elegans spe-10(hc104) him-5(e1490) V. Show Description
Temperature sensitive, maintain at 16C. Segregates males. Average progeny at 16C is 7 +/- 5. Average progeny at 25C is 0.1 +/- .1.
BC14126 C. elegans dpy-5(e907) I; sEx14126. Show Description
sE14126 [rCesC34D1.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). sEx14126 [rCesC34D1.3::GFP + pCeh361].
BC4923 C. elegans egl-38(sy294) IV; him-5(e1490) V. Show Description
BE148 C. elegans rol-9(sc148) V. Show Description
Strong right roller.
BE149 C. elegans rol-9(sc149) V. Show Description
Strong right roller.
BL5717 C. elegans inIs179 II; him-8(e1489) IV. Show Description
inIs179 [ida-1p::GFP]. GFP is expressed in a subset of neurons and the neuroendocrine uv1 cells of the vulva. Identified neurons include ADE, ALA, ASI, ASK, AUA, ASG, AVH, AVJ, AVK, VC, HSN, PDE, PVP, PHA, PHB, PHC. Male sex-specific neurons include CA and some ray neurons. Plasmid pida-1::GFP 7.6 was injected into N2 and integrated to generate BL5715, then crossed into him-8(e1489) background.
BW1199 C. elegans him-8(e1489) IV; unc-42(e270) sma-1(e30) V; ctDp8[vab-8(e1017)] (V;f). Show Description
BW1434 C. elegans dpy-17(e164) III; him-8(e1489) IV; her-1(y101hv1) unc-42(e270) V; ctDp11 (III;V;f). Show Description
Wild type hermaphrodites and males which produce WT, Dpy, Unc and DpyUnc progeny due to mating or loss of the duplication. Pick WT hermaphrodites and males to maintain.
BW1809 C. elegans gpa-16(it143) I; him-5(e1490) V. Show Description
Temperature-sensitve. Maintain at 15C. Slight Maternal Effect Lethal (Mel) at 15C, more pronounced at 20C. Highly penetrant Mel at 25C and a fraction of the survivors have reversed left-right organs.
BW1935 C. elegans unc-119(ed3) III; ctIs43 him-5(e1490) V. Show Description
ctIs43 [dbl-1p::GFP + dbl-1p::GFP::NLS + unc-119(+)].
BW538 C. elegans unc-13(e1091) lin-10(e1438) I. Show Description
Unc and Vul.
BW589 C. elegans lin-10(e1438) unc-29(e1072) I. Show Description
Vul and Unc.
BW635 C. elegans lin-10(e1438) daf-8(e1393) I. Show Description
Maintain at 15C. Vulvaless. Temperature-sensitive constitutive dauer (Daf-c) at 25C.
CB1402 C. elegans unc-15(e1402) I. Show Description
Temperature sensitive Unc-paralyzed. WT at 15C. leaky at 25C. Sterile at 25C.
CB1408 C. elegans unc-83(e1408) V. Show Description
Marginally Unc-cannot back. Lethargic. Migration pre VC defect. M-MATING+POOR <1%WT.
CB1410 C. elegans unc-84(e1410) X. Show Description
Marginally Unc-kinky backing. Temperature sensitive. Migration pre VC defect. M-MATING+POOR <1%WT.
CB1413 C. elegans lin-7(e1413) II. Show Description
Vulvaless. Incomplete penetrance. Recessive. Males can mate.