Search Strains - Caenorhabditis Genetics Center (CGC)

More FieldsRestrict search to strains with endogenously-tagged loci

Strain	Species	Genotype
SJ4197	C. elegans	zcIs39 II. Show Description zcIs39 contains [dve-1p::dve-1::GFP].
SJ4199	C. elegans	zcIs40 X. Show Description zcIs40 [dve-1p::dve-1::3xmyc-His tag + myo-3p::GFP].
OP398	C. elegans	unc-119(tm4063) III; wgIs398. Show Description wgIs398 [dve-1::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)
PHX7515	C elegans	dve-1(syb7515[dve-1::AID::mScarlet]) X. Show Description AID::mScarlet tag inserted into endogenous dve-1 locus via CRISPR/Cas9 engineering. Reference: Alexander, K.D. et al. Nat Commun 2023 Nov 18;14(1):7520. PMID: 37980357.
RW10611	C. elegans	unc-119(ed3) III; zuIs178 V; stIs10024; stIs10692. Show Description zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)]. stIs10692 [dve-1::H1-wCherry + unc-119(+)].
RW11501	C. elegans	unc-119(tm4063) III; stIs11501. Show Description stIs11501 [dve-1::H1-wCherry + unc-119(+)].
SJ4203	C. elegans	zcIs39 II; zcIs41 V. Show Description zcIs39 contains [dve-1p::dve-1::GFP]. zcIs41 contains [ubl-5p::3xmyc-His tag::ubl-5 + myo-3p::GFP].