| RB934 |
C. elegans |
C41C4.7(ok813) II. Show Description
C41C4.7 Homozygous. Outer Left Sequence: ACTGTCGGATCCATGACACA. Outer Right Sequence: CCGTTCTCTCGGTCAATCAT. Inner Left Sequence: GTCCGCTGGTTTTCACATCT. Inner Right Sequence: GTGGCATTTTTGCTCGTTCT. Inner Primer PCR Length: 2465. Estimated Deletion Size: about 2000 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
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| KG2430 |
C. elegans |
ceIs56 X. Show Description
ceIs56 [unc-129p::ctns-1::mCherry + nlp-21p::Venus + ttx-3p::RFP]; Maps to X: 9.0 +/ 3.0 m.u. Expresses the lysosomal membrane marker CTNS-1 in a subset of 9 DA/DB cholinergic motor neurons in the ventral cord, and NLP-21::Venus in the same neurons as a marker for soluble DCV cargo and to help identify the boundaries of the somas and axons when imaging lysosomes. Reference: Edwards SL, et al. Genetics. 2015 Sep;201(1):91-116. Edwards SL, et al. Genetics. 2015 Sep;201(1):117-41.
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| PHX5270 |
C. elegans |
ctns-1(syb5270[ctns-1::wrmScarlet]) II. Show Description
wrmScarlet tag inserted at C-terminus of endogenous stns-1 locus. Lysosomal marker. Reference: Ngale Njume F, et al. iScience. 2022 Oct 14;25(11):105357. doi: 10.1016/j.isci.2022.105357. PMID: 36339267.
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| WEH690 |
C. elegans |
sam-4(syb4007[sam-4::mScarlet-I]) ctns-1(syb5851[ctns-1::mCitrine]) II. Show Description
mScarlet-I tag inserted into the C-terminus of endogenous sam-4 locus. mCitrine tag inserted into the C-terminus of endogenous ctns-1 locus. Punctate expression in early embryos. Green fluorescence can be seen throughout development, but is dimmer than the mScarlet version. Reference: Fazeli G, et al. Curr Biol. 2023 Feb 27;33(4):607-621.e7. doi: 10.1016/j.cub.2022.12.041. PMID: 36652947.
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