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Strain Species Genotype
RG3442 C. elegans col-58(ve942[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) I. Show Description
Homozygous viable. Deletion of 643 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: cattacaccgtaattacactttatcacct ; Right flanking sequence: AGGTGATGACATATCAGTGCTTCGTCACGA. col-58 sgRNA A: GGAGTTCCGGGATCATCAGG; col-58 sgRNA B: aaaggaaggatgggttggtg. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.