clec-87(sy1396) I. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of clec-87.
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
left flanking sequence: GTTTTTGCCTTCTCGTTGCTTTCATCCTTCCTGGG
right flanking sequence: CTATTCCTCGTTCATGCAGCTCCGACTTCTTCAAC
inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: GCATGAACGAGGAATAGCCC
Method Reference: G3 (Bethesda).
|