Search Strains

bsIs2 [pie-1::GFP::spd-2 + unc-119(+)]. ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Wild type animals express GFP marked DNA (GFP-H2b) and centrosomes (GFP-SPD-2) in germ line and embyros. Maintain at 24C to obtain optimal GFP expression.

bsIs20 [tbb-1p::mCherry::tbb-2::tbb-2 3′UTR + unc-119(+)]. Maintain at 20C or lower. dyn-1(luv35) is a CRISPR-engineered mutation reproducing the dyn-1(ky51) allele. GFP inserted at the C terminus of endogenous plk-1 locus. At 26C, embryos exhibit elevated centrosomal PLK-1::sfGFP levels, persistence of PLK-1::sfGFP at centrosomes during late mitosis, and mild defects in intercellular bridge (midbody) assembly. Strain expressing mCherry::tubulin and endogenously tagged PLK-1::sfGFP enables live imaging of spindle dynamics and centrosomal PLK-1 localization during mitosis in embryos. Reference: Dierlam C, et al. Mol Biol Cell. 2026 Apr 1;37(4):br10. doi: 10.1091/mbc.E25-07-0337. PMID: 41705964.

bsIs20 [tbb-1p::mCherry::tbb-2::tbb-2 3′UTR + unc-119(+)]. GFP inserted at the C terminus of endogenous plk-1 locus. Strain expressing mCherry::tubulin and endogenously tagged PLK-1::sfGFP enables live imaging of spindle dynamics and centrosomal PLK-1 localization during mitosis in embryos. Reference: Dierlam C, et al. Mol Biol Cell. 2026 Apr 1;37(4):br10. doi: 10.1091/mbc.E25-07-0337. PMID: 41705964.