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Strain Species Genotype
ABR156 C. briggsae Cbr-she-1(v35) IV; mfIs42. Show Description
mfIs42 [Cel-sid-2(+) + Cel-myo-2::dsRed]. Maintain at 15C. Feminization is partially-penetrant at 15C; most hermaphrodites are somewhat self-fertile and can lay small broods. Can be maintained by crossing with male siblings. Feminized C. briggsae strain made susceptible to RNAi knock-down by feeding dsRNA due to the transgenic expression of C. elegans SID-2. Generated by crossing parental strains JU1018 with RE665. Reference: Booth LN, eLife 2019 Jul 8;8:e46418. PMID: 31282863.
AF16 C. briggsae Show Description
See WBG 7(1) 48. Isolated from soil in Ahmedabad, India. Previously called C. briggsae G16.
BC1972 C. briggsae Cbr-unc(s1270). Show Description
C. briggsae strain. Twitcher. Hets twitch only in prescence of nicotine.
BC1977 C. briggsae Cbr-unc(s1275). Show Description
C. briggsae strain. Unc-tends to coil.
BC1983 C. briggsae Cbr-dpy(s1281). Show Description
C. briggsae strain. Chubby. Moves poorly.
BW1850 C. briggsae Cbr-him(s1290). Show Description
C. briggsae strain. Hermaphrodites are variably self-sterile, which is overcome by mating to males of the same genotype. From BC1991.
BW287 Panagrolaimus sp. Show Description
Chinese rhabditis hermaphrodite nematode from Bill Wood's Chinese collection, Beijing. According to David Fitch this strain is Panagrolaimus sp. April 2008: According to sequence data from Howe & Denver 2008 BMC Evol Biol 8:62, strain BW287 is C. briggsae.
CA998 C. elegans ieDf2 [unc-119+]/mIs11 IV. Show Description
mIs11 [myo-2p::GFP + pes-10p::GFP + F22B7.9::GFP]. Heterozygotes are wild-type with dim GFP signal in the pharynx. mIs11 homozygotes are wild-type with bright GFP in the pharynx. ieDf2 homozygotes (non-GFP) develop normally but produce 97.5% inviable embryos and a high frequency of males among the surviving self-progeny. Pick WT with dim GFP+ in pharynx to maintain. mIs11 homozygotes will quickly overtake the population if not selected against. GFP expression in 4-cell embryos, pharyngeal muscle and gut. ieDf2 is a deficiency of zim-1, zim-2, zim-3, and him-8 generated by MosDel, resulting in single-copy insertion of a copy of the C. briggsae unc-119 gene on Chromosome IV. The deletion spans the sequences from the beginning of the zim-1 coding sequence through the ttTi22866 Mos1 insertion site.
CB4507 C. elegans Show Description
Wild isolate from Palm Canyon, CA. Segregated to remove gro-1(e2400) [CB4512]. Caenorhabditis elegans wild isolate CB gro-1(+) subclone of PaC1 (Tc1 pattern IV).
CP101 C. briggsae Cbr-puf-2(nm66)/Cbr-dpy-?(nm4) II. Show Description
Larval-lethal puf-2 deletion allele. Heterozygotes are WT (slightly Dpy) and segregate 25% Dpy, 50% wild-type heterozygotes, and 25% larval lethal (arrest L1-L2). Maintain by picking WT and checking for correct segregation of progeny. Map distance between nm4 and nm66 has not been preciely determined, but is tight enough that >90% of non-Dpy non-Lva progeny from double-heterozygotes retain the parental genotype. Reference: Liu Q & Haag ES. J Exp Zool. 2013 Part B.
CP161 C. briggsae Cbr-unc-119(nm67) III; nmIs7. Show Description
nmIs7 [Cni-mss-1(+) + Cni-mss-2(+) + Cbr-myo-2::GFP + unc-119(+)]. Insertion site of transgene is not known, but it is not in LG III or X. Males with this transgene are more competitive in siring progeny; also a higher ratio of males in the population. Derived from parental strain CP99, which in turn was derived from AF16. Reference: Yin D, et al. Science. 2018 Jan 5;359(6371):55-61.
CP3 C. briggsae Cbr-dpy-?(nm3) X. Show Description
X-linked Dpy.
CP36 C. briggsae Cbr-fem-2(nm27) III. Show Description
XX animals have no obvious phenotype: self-fertile with normal brood size. XO animals are self-fertile hermaphrodites with low brood size and some somatic gonad defects. Cbr-fem-2/+ XO animals show late-onset germline feminization. AF16 was the parental strain.
CP38 C. briggsae Cbr-tra-1(nm2)/Cbr-let(nm28) III. Show Description
When singled, hermaphrodites should throw 2/3 hermaphrodites and 1/2 nm2 XX males. The lethal appears to balance the nm2 allele pretty well, but precise recombination mapping has not been performed. The XX males maintain their phenotypic resemblance to the unbalanced strain and are probably not fertile due to obvious gonadal deficiencies. This strain has been successfully grown at 15C and 20C. Both strains appear to have complete penetrance of the mutant phenotypes.
CP4 C. briggsae Cbr-dpy-?(nm4) II. Show Description
Dpy. Tightly linked to Cbr-tra-2 on LG II. Molecular identity unknown. Reference: Kelleher DF, et al. Genetics. 2008 Mar;178(3):1415-29.
CP87 C. briggsae Cbr-fem-3(nm63) IV. Show Description
XO are self-fertile hermaphrodites with low brood size and some somatic gonad defects. XX animals have normal brood size.
CP89 C. briggsae Cbr-fem-2(nm27) III; Cbr-fem-3(nm63) IV. Show Description
XX are self-fertile hermaphrodites.
CP99 C. briggsae Cbr-unc-119(nm67) III. Show Description
Derived from AF16. Outcrossed >6x to AF16. Unc, slightly Dpy, no dauer formation (similar to C. elegans unc-119). nm67 is a deletion (827 bp) in Cbr-119 begining in exon 1 and ending 3' of exon 4. Reference: Liu Q, et al. Development. 2012 Apr;139(8):1509-21.
DH1300 C. briggsae Show Description
DH subclone of C. briggsae Zuckerman. This stock was maintained in liquid culture for some number of years, and has acquired mutations that have not been named or mapped. It is Unc, dauer-defective and ts lethal. Previously called C. briggsae BO.
DR1690 C. briggsae C. briggsae. Show Description
Previously called C. briggsae Zuckerman. This stock was maintained in liquid culture for some number of years and carrues a 33-kilobase deletion that disrupts one of the srg paralogs, CBG24690, and six other genes. It is Unc, dauer-defective and ts lethal. Reference: McGrath PT, et al. Nature. 2011 Aug 17;477(7364):321-5.
DY31 C. briggsae Cbr-lev-?(sy5440) I. Show Description
ED3032 C. briggsae Show Description
Isolated as a single hermaphrodite from a flower bed soil sample in the botanic gardens in Chungcheng, Taipei, Taiwan, September 29, 2005.
ED3033 C. briggsae Show Description
Isolated as a single hermaphrodite from rotting wood in the garden of a private residence in Tien Mu, Taipai, Taiwan, Oct. 2, 2005.
ED3034 C. briggsae Show Description
Isolated as a single hermaphrodite from rotting wood in the garden of a private residence in Tien Mu, Taipai, Taiwan, Oct. 2, 2005.
ED3035 C. briggsae Show Description
Isolated as a single hermaphrodite from rotting wood in the garden of a private residence in Tien Mu, Taipai, Taiwan, Oct. 2, 2005.
ED3036 C. briggsae Show Description
Isolated as a single hermaphrodite from rotting wood in the garden of a private residence in Tien Mu, Taipai, Taiwan, Oct. 2, 2005.
ED3037 C. briggsae Show Description
Isolated as a single hermaphrodite from rotting wood in the garden of a private residence in Tien Mu, Taipai, Taiwan, Oct. 2, 2005.
ED3083 C. briggsae Show Description
Caenorhabditis briggsae wild isolate. Isolated from compost from Jenny Pettifor's garden in the Paview neighborhood in Johannesburg, South Africa, on May 5, 2006. Landscape: Urban_garden. Isolated from a compost sample from a private garden in Johannesburg, South Africa, March-April 06 by E. Dolgin. Same compost sample as ED3078-3089. WBPaper00035666. GPS: -26.200001 28.000000, Johannesburg, South Africa. Substrate: compost_heap. Sampled_by: Elie Dolgin WBPerson12345. 2006.
ED3092 C. briggsae Show Description
Caenorhabditis briggsae wild isolate. Isolated from leaf litter from the Uhuru Gardens public park in the south part of Nairobi, Kenya on May 17, 2006.
ED3101 C. briggsae Show Description
Caenorhabditis briggsae wild isolate. Isolated from leaf litter from the Uhuru Gardens public park in the south part of Nairobi, Kenya on May 17, 2006.
EG4181 C. briggsae Show Description
Isolated by Michael Ailion from rotting apricot from home of Wayne Davis and Danielle Endres in Salt Lake City, Utah, 8/4/2006, under tree on north side of house. Coordinates: 40° 42' 26.16" N, 111° 52' 3.27" W. Animals move very rapidly. Strain started from a single L4 hermaphrodite and grown three generations before freezing. 18S rDNA sequence differs from C. briggsae NCBI entry (PB102), but is identical to the 18S sequence of AF16, HK104, PB800 and VT847. Cross-fertile with AF16.
EG4207 C. briggsae Show Description
Mixed "strain" started from many different worms that emerged from the same apricot that gave rise to strain EG4181. Some worms emerged as dauers, but there were also L4s and adults. Consists of a mix of the worms that came from the apricot to try to maintain as much diversity as possible in the population and thus is not a true strain in the traditional sense. It will be sent as contaminated worms since it cannot be cleaned up without going through a bottleneck.
EG9814 C. elegans unc-119(ox819) III. Show Description
Crispr/Cas9 engineered mutation in unc-119. ox819 is an 11 bp deletion causing a frameshift after V110 (UNC-119a) and then appends 29 out-of-frame amino acids before a stop codon. unc-119(ox819) animals are phenotypically identical to unc-119(ed3) animals and can be rescued by expression of the smaller C. briggsae unc-119 gene (Cbr-unc-119). Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https://doi.org/10.1101/2021.08.03.454883
HK104 C. briggsae Show Description
C. briggsae wild type strain collected from Okayama, Japan.
HK105 C. briggsae Show Description
C. briggsae wild type strain collected in Sendai, Japan.
JEL446 C. briggsae Cbr-met-2(xoe1) III. Show Description
Derived from C. briggsae strain AF16. Reference: Larson BJ, et al. Genetics. 2016 Jun 8. pii: genetics.116.191130.
JEL447 C. briggsae Cbr-met-2(xoe2) III. Show Description
Inappropriate targeting of H3K9me2 and H3K9me3. Derived from C. briggsae strain AF16. Reference: Larson BJ, et al. Genetics. 2016 Jun 8. pii: genetics.116.191130.
JU1018 C. briggsae mfIs42. Show Description
mfIs42 [Cel-sid-2 + Cel-myo-2::DsRed]. JU977 integrated with gamma-rays.
JU1038 C. briggsae Show Description
Isolated from rotting pears sampled below their tree on 15 Oct 2006 in Le Blanc (Indre, France). Sample plated on 16 Oct 2006. Picked as an adult on 18 Oct 2006. PrA1.
JU1085 C. briggsae Show Description
Isolated by Marie-Anne Felix from leaf litter/bark/soil sampled on March 14, 2007, in the woods behind the parking lot of the Kacho-en aviary in Kakegawa, Shizuoka prefecture, Japan (ca. 100 m from the aviary and 200 m from the vegetable gardens).
JU1341 C. briggsae Show Description
Hermaphrodite. Isolated by MAF from rotting small red fruits (same as JU1342) and bark in the forest near the top of the mountain in Ponmudi, Kerala, India on 22 Dec 2007 (ca. 20 m from JU1340).
JU1345 C. briggsae Show Description
Hermaphrodite. Isolated by MAF from dark brown "fruits" (1x1.5 cm) on fallen tree trunk in the forest near the top of the mountain in Ponmudi, Kerala, India on 22 Dec 2007.
JU1348 C. briggsae Show Description
Hermaphrodite. Isolated by MAF from a mix of rotting fruits, leaf litter, soil, bark, flowers, etc. sampled in a ca. 3 km wide area in Periyar Natural Preserve, Kerala, India on 28 Dec 2007. Isolated from a plate containing a rotting fruit similar to that of sample #46 - JU1361/2).
JU279 C. briggsae Show Description
From Jardin des Plantes, Paris, France, 6 Aug 02. Isolated from a decomposing Helix aspersa snail containing maggots (same snail as JU280, JU296).
JU348 C. briggsae Show Description
From Merlet, Lagorce (Ardèche), France. 8 Sep 02. From a Oxychilus sp. snail , under the mulberry tree.
JU372 C. briggsae Show Description
From Viosne Valley, next to Santeuil, Val d'Oise. France. Isolated from embankment on the road, below a cultivated field. 22 Sep 02.
JU403 C. briggsae Show Description
From Hermanville (Calvados), France, from a compost pile, Sept 2002.
JU439 C. briggsae Show Description
From ReykjavIk cemetery (Iceland). Collected on August 10, 2003.
JU441 C. briggsae Show Description
From Beauchêne (Eure & Loir -France), in the cabbage parcel, 12 Sep 03.
JU516 C. briggsae Show Description
Isolated from compost heap in Marsas (Hautes-Pyrénées, France) on August 15, 2004.