| JH3475 |
C. elegans |
meg-3(ax3055) meg-4(ax3052) X. Show Description
Embryonic P granules not segregated, 30% maternal effect sterility on average, RNAi insensitivity. meg-3(ax3055) and meg-4(ax3052) are precise deletions of the entire coding sequence made by CRISPR/Cas9, designed to be cut again to make insertions at the endogenous locus. References: Smith, J. et al. Elife. 2016 Dec 3;5:e21337. doi: 10.7554/eLife.21337. PMID: 27914198 Ouyang JPT, et al. Dev Cell. 2019 Sep 23;50(6):716-728.e6. PMID: 31402283 |
|
| JH3477 |
C. elegans |
meg-3(ax3051[meg-3::OLLAS]) meg-4(ax3052) X. Show Description
P granule size reduced, detection of MEG-3 by anti-OLLAS antibody. References: Smith, J. et al. Elife. 2016 Dec 3;5:e21337. doi: 10.7554/eLife.21337. PMID: 27914198 Schmidt H, bioRxiv 2020.10.15.340570 (2020) doi:10.1101/2020.10.15.340570. |
|
| JH3861 |
C. elegans |
meg-3(ax4502[meg-3(F705A,Y708A,Y713A, N725A)::OLLAS]) meg-4(ax3052) X. Show Description
20% Maternal effect sterility on average, embryonic P granule defect, RNAi insensitivity. Engineered mutations in the endogenous meg-3 locus disrupt the binding and localization of PGL proteins to P granules in the early embryo while MEG-3 itself is still forms an asymmetric cytoplasmic gradient and granules. References: Smith, J. et al. Elife. 2016 Dec 3;5:e21337. doi: 10.7554/eLife.21337. PMID: 27914198 Schmidt H, bioRxiv 2020.10.15.340570 (2020) doi:10.1101/2020.10.15.340570. |
|
| JH3929 |
C. elegans |
pos-1(tn1730[gfp::3xflag::pos-1]) V; meg-3(ax3055) meg-4(ax3052) X. Show Description
Allows visualization of POS-1 protein in the absence of embryonic P granules. Derived by crossing parental strains DG4222 and JH3475. Reference: Simmons WR, et al. 2026 bioRxiv 2026.07.01.735846; doi: https://doi.org/10.64898/2026.07.01.735846. |
|
| JH4878 |
C. elegans |
nos-2(egx211[7xHA::nos-2]) II; meg-3(ax3055) meg-4(ax3052) X. Show Description
7xHA tag inserted at N-terminus of endogenous nos-2 locus. Embryonic P granules fail to segregate. 30% maternal effect sterility on average. RNAi insensitivity. Derived by crossing parental strains JH3475 and JH4876. NOTE: egx211 was originally designed as 8xHA tag but sequencing confirmed a point mutation disrupts one repeat, making it a functional 7xHA tag. egx211 CRISPR Guide sequence: TTCTTCTTGAAAGATGTCTC. Reference: Simmons WR, et al. 2026 bioRxiv 2026.07.01.735846; doi: https://doi.org/10.64898/2026.07.01.735846. |
|
| JH5098 |
C. elegans |
xnd-1(ax4606[8xAlfa-Tag::xnd-1]) III; meg-3(ax3055) meg-4(ax3052) X. Show Description
8xAlfa-Tag inserted at N-terminus of endogenous xnd-1 locus. Embryonic P granules fail to segregate. 30% maternal effect sterility on average. RNAi insensitivity. Derived by crossing parental strains JH3475 and JH4940. ax4606 CRISPR Guide sequence: AATGGGTTCAGAAGACATCC. Reference: Simmons WR, et al. 2026 bioRxiv 2026.07.01.735846; doi: https://doi.org/10.64898/2026.07.01.735846. |
|
