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Strain Species Genotype
VC728 C. elegans atl-1(ok1063) V. Show Description
T06E4.3. Superficially wild type. External left primer: TGAAGCTGGTCCTCTGTCTG. External right primer: AAGAAATGAGTGCCGCAAGT. Internal left primer: TATATCACGGTCTCCTCGGG. Internal right primer: TCTTCAATCTCGCCAAATCC. Internal WT amplicon: 3344 bp. Estimated deletion size: 1400 bp. Breakpoints only narrowed due to extremely poor sequence quality. Deletion of approximately 1400 bp lies between chromosome V coordinates 9640245 and 9642180. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
DW101 C. elegans atl-1(tm853) V/nT1 [unc-?(n754) let-? qIs50] (IV;V). Show Description
Heterozygotes are Unc and GFP+ with signal in the pharynx. atl-1(tm853) homozygotes are non-Unc, viable, GFP-, and produce 100% dead embryos. qIs50 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter (F22B7.9) driving GFP in the intestine. nT1[unc-?(n754) let-? qIs50] is also known as DnT1[qIs50]. qIs50 is apparently inserted on DnT1. qIs50 is somewhat dimmer than the similar qIs51.