C08B11.3. Homozygous sterile deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP gk1041 homozygotes (sterile adult). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: ATCTCGATCGACACTTCGCT. External right primer: TAACATATCGACGTTGGGCA. Internal left primer: ACGGCTCGTCTGTTCTGATT. Internal right primer: AATTGACGGATCCACCTGAG. Internal WT amplicon: 2394 bp. Deletion size: 561 bp. Deletion left flank: GATCAATCTGAAATTCATTTTTCAAATTAT. Deletion right flank: TGCAAAATCGATTTCGTTCGGCAATCCAGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F52B11.3. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok3197 homozygotes (probable embryonic arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GGCTTAGGCACAGACGTAGG. External right primer: CACTGAGAGCGAGACGACTG. Internal left primer: GCACTGCTTCTTCCTGCTTC. Internal right primer: CAGAGAAGCTCGGTGGAGTC. Internal WT amplicon: 1129 bp. Deletion size: 806 bp. Deletion left flank: TGAATCCCAATTCGGAGGAATGGCTCTCTG. Deletion right flank: AGGAGAAGCTTTCGGCTATCATTCGAGTAT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

C13F10.4. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok3257 homozygotes (late larval arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GAAGGACTCCTGATGACCGA. External right primer: CGTGGGCAGAGGTTTATGTT. Internal left primer: TCATCTGGCTGCTGACTGAC. Internal right primer: TGACTACAATGGAAGTGGTTCA. Internal WT amplicon: 1092 bp. Deletion size: 525 bp. Deletion left flank: TTTTCCACCTTCTTCGCCAGCGAACAATAC. Deletion right flank: GTTGAGAAATTGATCGGAGTAATGGGCAGG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

K02E11.10. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok3266 homozygotes (probable embryonic arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: AGCAACTTGTCCTTGTTGGG. External right primer: GGAGTGTGCAGCAAATTTCA. Internal left primer: CTTCGAAGCCTCCTTGAGTA. Internal right primer: GCGTCTTGAGGCCATAGTTC. Internal WT amplicon: 1208 bp. Deletion size: 582 bp. Deletion left flank: CCTGAGCAGGCCCTTGCTGATATCCGGCTC. Deletion right flank: GGCAGGCTAAGATCACAACGGATTTCATCT. Insertion Sequence: TTCCCTGAACTCCTTGAGCAGATCCCT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

T08G5.5. Homozygous sterile deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok2442 homozygotes (sterile, eggs don't hatch). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CTTTGACATTGCACATTGGG. External right primer: CATACACGCCTTGCGAAGTA. Internal left primer: GGTAGTTTGAATCACCGCGT. Internal right primer: CTTGTTAGCCGGTGGAAGAG. Internal WT amplicon: 2794 bp. Deletion size: 1397 bp. Deletion left flank: CCACGACGGCTTTTCGATTTAAATTTCTAG. Deletion right flank: TTTCGAACAGAAAAGAATACCATTTCACCA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

K04C2.6. External left primer: ATTCCCAAATTCGAAGAGCA. External right primer: CCATCACAACACCACCACAT. Internal left primer: AAATCTCATTATGACAACGAACAAA. Internal right primer: GCATCCAATCCATGCAATTA. Internal WT amplicon: 1203 bp. Deletion size: 580 bp. Deletion left flank: GTGTGGTCTACAGTAAAAGAGCCCAAAGAA. Deletion right flank: GTATCATTGTAAATTGTATAACTCAATTGT. Insertion Sequence: AAGAGCTACAAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F08F1.5. Apparent homozygous lethal deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok3213 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: CAAATATCAGCACCAATGCG. External right primer: TGCAATGTGCTCCTATGCTC. Internal left primer: CTTACCTGCAAAACCGCTTC. Internal right primer: CAATCTTTCATTTTTGGGCG. Internal WT amplicon: 1179 bp. Deletion size: 649 bp. Deletion left flank: CTTTCTCAATCTTACCTGCAAAACCGCTTC. Deletion right flank: GTGACCGCAAACTGATTAGTCTCTTGAAAT. Insertion Sequence: ACCGCAAAC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F07A5.1. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok3267 homozygotes (sterile, no eggs). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CAAAAACCAACCGGTTCAAG. External right primer: ATCACCAAACCGTTCAAAGC. Internal left primer: CTTGAAAAGAGCACCGATGA. Internal right primer: GGTGCTAAACAACATTTCGGA. Internal WT amplicon: 1264 bp. Deletion size: 544 bp. Deletion left flank: ATTAAAAGAATTCCGTCGGCACACAGGTAC. Deletion right flank: CACGAATCAACGTCCAAGAATTTGCAAACC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F09F7.7. External left primer: GGTGTCCATCCCAATCAATC. External right primer: CGATCACATTCGTCAAATCG. Internal left primer: CGAGCCGTTCACTCCTTATC. Internal right primer: CAATTCCAACGACAAATCTGAA. Internal WT amplicon: 1340 bp. Deletion size: 939 bp. Deletion left flank: GACAAAACAGACAGAAAAAAGGAATTATCA. Deletion right flank: TTCAGATTCTGACAGAAAATTGTGAATGAG. Insertion Sequence: GA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

B0513.1. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok3326 homozygotes (late larval arrest or sterile adult, tends to explode). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CCAAGTCCTTCCACCGTCTA. External right primer: TTGATCAAGCACGACAAAGC. Internal left primer: AACAGAGAGCCGACACCATC. Internal right primer: TCTACGGCATTGCAGTGTTC. Internal WT amplicon: 1385 bp. Deletion size: 707 bp. Deletion left flank: TATGATCTCTATGATCTACCAAGGTTAGCC. Deletion right flank: AGTCGCTGTTCGACTACTACGGTAGCCCAG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F45G2.6. Apparent homozygous lethal deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes), and ok1721 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: GTGGATTACCAGCGGACTGT. External right primer: AGAGGCAAAAGCAATGATGG. Internal left primer: TCCGATCAAGCTGAACTGTG. Internal right primer: CGCCTTTTCTCCCCTACTTC. Internal WT amplicon: 2848 bp. Deletion size: 954 bp. Deletion left flank: AAAACGGGGGTGAAGCCATTTACTTTCAGG. Deletion right flank: ATTTGGTTATAAGATGATGGCTTGTGCATG. Insertion Sequence: TCTTTC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

T25B6.2. External left primer: TGCATGGATCTTCTCACTGC. External right primer: TCTGGGTACATTGGGCTACC. Internal left primer: GCCATACGGAACTGGATACG. Internal right primer: AACCTGGTTGGTTCTGCATT. Internal WT amplicon: 1196 bp. Deletion size: 686 bp. Deletion left flank: TGTGCAAAGTTGAAAAAATGGTAACTTTTT. Deletion right flank: CTCAATTTAGTACACAATGTTGCCCAGAAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

K07E3.7. External left primer: GACAGGAAAACTCCAGCCAG. External right primer: GATCGTCATGGAGAACCGAT. Internal left primer: CGAGGAGAATAAAGCGCATC. Internal right primer: TCAATGAACTTTCTGTTGCCA. Internal WT amplicon: 1344 bp. Deletion size: 366 bp. Deletion left flank: CGGTCGTCTGAATCGTTTTCACCATTTAAC. Deletion right flank: GGCACACAACGAATATCTGGGACGTTGGAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

R09E12.3. External left primer: TCCCTATTTTCAGGCGATTG. External right primer: CGACGTCACCAAAGAAGACA. Internal left primer: GATCTCGGAAATGCTGCCTA. Internal right primer: AAGACGAGTCCAGAGGACGA. Internal WT amplicon: 1160 bp. Deletion size: 336 bp. Deletion left flank: TGAGTGGTCGAAGGCTCAGCGAGCCTACGA. Deletion right flank: CCCCATGGTCTGTGTTTTTTGCATCTCGCT. Insertion Sequence: G. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

Y75B8A.24. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok3320 homozygotes (grotty sterile with vulval blip). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CCTTCCCCTACCTAACAGCC. External right primer: GAGAAGGAAGTTGTCGGTGG. Internal left primer: ACAGAAGCTCATCTGCCGAG. Internal right primer: ACGTCGCATCCTACTCGTCT. Internal WT amplicon: 1206 bp. Deletion size: 389 bp. Deletion left flank: CTCATCTGCCGAGTAACTTCTCAGCACTCT. Deletion right flank: CCAACGCTAGCGGATGGAGCCAAGCACTGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

R10D12.14. External left primer: AAGAGCGAGATGACGAGGAA. External right primer: ACCATTTGTCCGAGCAACTC. Internal left primer: GACATCAAAATACCGACGGC. Internal right primer: GAACACGAGAAGCCTGTTCC. Internal WT amplicon: 1196 bp. Deletion size: 595 bp. Deletion left flank: TCCAATGCCGCTTCTCCATCAAATGAATCA. Deletion right flank: ATGATTTTAAAATAGTTTCAGATTTCAAAG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

C26F1.6. External left primer: GCCTCAGCAAAATGTAAGCC. External right primer: GTTTGTCCTCGCGGTAGGTA. Internal left primer: GAACGTGATGGTGCTGATGT. Internal right primer: CCGCAAATCCCTTCTATCAG. Internal WT amplicon: 1147 bp. Deletion size: 591 bp. Deletion left flank: CGCACTTCTTATTGTGGTACCAGAATGTTG. Deletion right flank: AAAATACGGTCACACTCATAGACTGATAGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F23C8.6. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok3325 homozygotes (early larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GCGGGGTCAAAGTACACACT. External right primer: CCGCAGATACACACACATGA. Internal left primer: CCGAAATTCGAAATTTTAGCC. Internal right primer: TTTTTATCGCTTTCTTGCGAA. Internal WT amplicon: 1309 bp. Deletion size: 702 bp. Deletion left flank: GTCGGCAGAGCAGTTGGCACGTTTGACGGA. Deletion right flank: GTGCTTTTATAAGTTAAATTTCGCAAGAAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

R07B7.16. External left primer: ATGGTGCATTGATGGTCAGA. External right primer: GCGGAAAACTCCAAACGATA. Internal left primer: CTTCCTGAATCAGCACAGCA. Internal right primer: GGCCCGTCTTGTTAACTTCA. Internal WT amplicon: 2735 bp. Deletion size: 1749 bp. Deletion left flank: TACTGTGAATATTGAACTGATCCATGAAAT. Deletion right flank: ATAGAGTTTCAGCTGTCCCTGGTCTCACAT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F22B8.6. External left primer: TCTCGTGCAGGGTGTAACTG. External right primer: CAATTCGCATCACATCCAAG. Internal left primer: CCCCTAGATTCAATGCCAAA. Internal right primer: CTTTTCCCATGCCGGTTTAT. Internal WT amplicon: 1283 bp. Deletion size: 508 bp. Deletion left flank: CAAAAAAGTGACACCCACCCATCTTTTCTT. Deletion right flank: TTATGCTCGTGGTGGAAACCCAACACGCGA. Insertion Sequence: ACGTT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

Y92H12A.2. External left primer: AAATACAATGCTCTCGCGCT. External right primer: GTAAGCGGCAAACGATTTTT. Internal left primer: TCTACGGGTCCGTCTATTGC. Internal right primer: ACTTCGAAACACTTTCCGGC. Internal WT amplicon: 1132 bp. Deletion size: 538 bp. Deletion left flank: AAAATTACAAGCTTTTAGAGGAAAAATTGA. Deletion right flank: GATTGTCTTATTTTGGCTTGATCTACGTTG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

K05F1.6. External left primer: ATCAATGCTCGGAGTGTTCC. External right primer: TCCGGTAGTGGCTTCTCACT. Internal left primer: TGTGCATGGAAATCACAGGT. Internal right primer: TTCTGGTAATACGAACACCAACA. Internal WT amplicon: 1188 bp. Deletion size: 472 bp. Deletion left flank: CTTCATGTCAATCATATTTATTTGTTCAAT. Deletion right flank: GGAGCAATTGAAATTCCAACATTGTTTGCG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

JC8.2. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok3322 homozygotes (early larval arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: TCGGCAAAATTGGATTTCTC. External right primer: ATTCTCGATGCTCCACCATT. Internal left primer: AATAATTCCGCAACGAAACG. Internal right primer: CAACATGATCCAGGAAACGA. Internal WT amplicon: 1109 bp. Deletion size: 478 bp. Deletion left flank: AAACTGCCGAGACCATAGGTAATGTAATTT. Deletion right flank: CTTCCATCATCAGACGAGCAGAACGCGGTG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

C44F1.2. External left primer: CAGGCTTGCATCGAATATGA. External right primer: GAACTGGAAGCTTCTGGGAA. Internal left primer: GCGACAGAAAGACTGGAAGG. Internal right primer: TGGTACGAACAGTACAGAACAAAAA. Internal WT amplicon: 1119 bp. Deletion size: 559 bp. Deletion left flank: CAACGGGCAGAGCTCCAGTTAACAAACGTC. Deletion right flank: CAGAAGCCGATTGTGAATCGAGTGCAATCG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F09G8.9. External left primer: ACTCGCCACGAAAAACATTC. External right primer: CCACCAAAACCACAGAAACC. Internal left primer: CAATTGCCACTCTCCTTTCC. Internal right primer: CAGTTCAGCCAACAATCGTG. Internal WT amplicon: 1085 bp. Deletion size: 668 bp. Deletion left flank: ACTCCTCTGAAAATTGAACCTCTTTTTGTT. Deletion right flank: TCTTCAATTAGGTATTTATAATTGAACGAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

Y75B8A.11. External left primer: AATGCCCAGCATTCTTCAAA. External right primer: TTGCCAGAGGAAAGCTCACT. Internal left primer: GCATGCACCTCAAAGAAAAA. Internal right primer: CGATGCTATTCGTGACCTGA. Internal WT amplicon: 1334 bp. Deletion size: 682 bp. Deletion left flank: CCACCAGCACCATAAACCATCAAAAGCTTG. Deletion right flank: GGCTGGGACTTTTTTGGTTTTTTTTTGTTG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

Y48A6B.8. External left primer: CGGAGACATCATGCTCATTG. External right primer: GGGACAGCACAGACAGATCA. Internal left primer: GACCGGTTGCACTGTAATGA. Internal right primer: AAATGGGCAACGTTGTGAAG. Internal WT amplicon: 1237 bp. Deletion size: 817 bp. Deletion left flank: GATTAAAAGTATTGAGTTACTTACAATCTG. Deletion right flank: CTTTGAAATTTTCGATGTCAGGGTGTGCAC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

D1007.7. External left primer: TTGTGGGGATTTTCTTGAGC. External right primer: TCATCATCGTCTCAACCGAA. Internal left primer: CGTGAGGCGACTGTAAATTG. Internal right primer: CTCCTACAAATCCACATCCG. Internal WT amplicon: 1364 bp. Deletion size: 582 bp. Deletion left flank: GTCCGAGCTGTTGATTTGGAAAACCTGGCG. Deletion right flank: ATCTCAGAGCAGATGATGGTCCAGCACCGA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

C10B5.1. Homozygous sterile deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok3270 homozygotes (sterile adult). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GCATCCCGATGATCTCATTT. External right primer: GCGTCAAATATGGTGAGCAA. Internal left primer: CATTTCATGGTTTTGCTCCA. Internal right primer: TTCTCAGAATTTAGTGTTTCCGT. Internal WT amplicon: 1224 bp. Deletion size: 573 bp. Deletion left flank: TCGTAGTGTGACGTCATTCTACAGTTTAGA. Deletion right flank: CAACAAAATCGAATCGAATTCTGGATGAAG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

Y54E2A.3. Apparent homozygous lethal deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes), and ok3305 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: AATTCGCTCAAAATCCATGC. External right primer: AAAATAAATGATGACGCGGG. Internal left primer: ATCAAAACAAATTCGGCCTG. Internal right primer: TTTTCACGAAAAATGTCGGTT. Internal WT amplicon: 1236 bp. Deletion size: 812 bp. Deletion left flank: CGCTGTATCTTTGGCGCGAAAATTTAGAAG. Deletion right flank: TTTAGCAATTTTTCAAAGCTTCTCACCATC. Insertion Sequence: CAATTTTTCAGCAATTTTAGCAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

B0035.11. Homozygous lethal or sterile deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok3436 homozygotes (late-larval to sterile adult arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain.External left primer: TTCGGACATAGTGGTTCCGT. External right primer: TTACGTTCCCCAGTGTCTCC. Internal left primer: AGCGAGGAAGACGTAGTGGA. Internal right primer: TGCACCAGGAACACCAGTTA. Internal WT amplicon: 1795 bp. Deletion size: 710 bp. Deletion left flank: GGAAGGTCATACGATGTGTAAGAACAGCTT. Deletion right flank: CTTTCGGCTTTCCTTCTCTGTCGGAATCAT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

ZK1037.10. External left primer: CTTTGTCAAGGCCTGAAAGC. External right primer: GTTTTCGACGACATCCCTGT. Internal left primer: TCACTTCTGACTGTGCCAGG. Internal right primer: CATGTGAGCCAGCTTTTCAA. Internal WT amplicon: 1238 bp. Deletion size: 701 bp. Deletion left flank: TCACACTGATATGTATTAGAACCCGCGGGG. Deletion right flank: AATGTTCTTCGCAATGTTACTAATGTAATC. Insertion Sequence: TTCTAATACATATCAG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F21F8.6. External left primer: CAAGAATCCAGGAAGGTCCA. External right primer: GAGAAGTGGAGAATGGGCAA. Internal left primer: TCCAAAATCCATTGGGAAGA. Internal right primer: ACCGTAATCCTTGGCAGCTA. Internal WT amplicon: 1299 bp. Deletion size: 680 bp. Deletion left flank: TGGGAAGAGAGCCATCACACACGTTCCATC. Deletion right flank: AATGTAGTGAATGCAAGACCGAATATTCCA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F29B9.5. External left primer: CTGTGAAGTATGCTGCCGAA. External right primer: TGGCAGGTACAATCTAGGGC. Internal left primer: TATTTCTGTATGCGGCAACG. Internal right primer: GGGCAAACCTAGAGAAAAACTATT. Internal WT amplicon: 1213 bp. Deletion size: 670 bp. Deletion left flank: CAACAATGTCCGAATTCATGAAAGGTGTGA. Deletion right flank: ATTATCACGTAAATATTTATATTTTAATAG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

C38D4.5. External left primer: TTTAAAAGCTTCAGCCGACC. External right primer:GCTGTCGTTCCGTCACTATG. Internal left primer: TCGGACGGACATTTTTCTTC. Internal right primer: ACAGAGCAACGGAAATTTGG. Internal WT amplicon: 3386 bp. Deletion size: 2724 bp. Deletion left flank: GAACAAAATGCGTGAATCTTTAGCTGATGA. Deletion right flank: AATTCCAATGTGAGTTTTTTTTTCAAAAAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

T06D4.4. External left primer: CGAATTGTGGCATGTTCTTG. External right primer: GGTAAATAGGCAGGCGTGAA. Internal left primer: GTCGAGTAATGTTGGCGAGC. Internal right primer: CATGAAGTAGGCACGCATGT. Internal WT amplicon: 1135 bp. Deletion size: 381 bp. Deletion left flank: AAGTAAGGGCCATCAATGATCGGAGCAATG. Deletion right flank: CGGAGAAAAAGCATTAAAACAACCTTTATC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

Y71H2AR.2. External left primer: AAAACCACTCCTTTGGTCCC. External right primer: CGTTGCAATGGGGTAAGTCT. Internal left primer: CCCAATTGGCGCTACTCTAA. Internal right primer: TTTTATACAAACGAAGAAGGCCTAA. Internal WT amplicon: 1156 bp. Deletion size: 794 bp. Deletion left flank: TATGGATAGTCTGCTTCTGTTTCTATGCCA. Deletion right flank: GAATTGAAGGTGCTTCTCGAGTTTGCTGGC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F57C12.1. External left primer: AAAGCCAGAACCAACCACTG. External right primer: ACTCAATGGCAAAAGATGCC. Internal left primer: CAACTACATATACAACGGCAATTC. Internal right primer: TTAATTCCTTTTTCCCTGCATT. Internal WT amplicon: 1213 bp. Deletion size: 442 bp. Deletion left flank: CCGCAAAAAAACAGTTCTTCACAAGAAAAC. Deletion right flank: AGTAAATACTATAAATTGTAGGAACGGATC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

W07E11.3. External left primer: TCAACTCTCACACAGCCCAC. External right primer: ATTTTCAGGTACACACCCGC. Internal left primer: GTTGGTTGAGATGCCACCTT. Internal right primer: CACAGAGCTTTCGTCTGACTC. Internal WT amplicon: 1219 bp. Deletion size: 372 bp. Deletion left flank: TTCCAAATATGTGTTTGGGTTTTAAGCTTG. Deletion right flank: CGACAATTGGTTTGGCAACGACTGACAATG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

T13H5.1. External left primer: TTTATGGCAAAAGCCATTCC. External right primer: CCACTGGAGCACAAAATCAA. Internal left primer: CAAAATTGAAGCTGCAGGAA. Internal right primer: GAAGTTCGAATTTGTTGCGTT. Internal WT amplicon: 1212 bp. Deletion size: 339 bp. Deletion left flank: TGTGTTACGTCGACGGAATGAGAATTTACT. Deletion right flank: TCATCATTTTGACTGCATGAAAAACGTCAA. Insertion Sequence: TTGAAATTTGAGTT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F20H11.1. External left primer: CAGCAACTCCATCAAAGCAA. External right primer: CGTTTCTGCCGATTTTTCAT. Internal left primer: AGTTGACAGAACTCCGGCAC. Internal right primer: TTTTGGCTAGAGAATCACAAAAA. Internal WT amplicon: 1279 bp. Deletion size: 472 bp. Deletion left flank: GTGCAAAAAAAACAATTTCTCCAGACCGGG. Deletion right flank: AAGGAGTTGAAGATTGATTATGAGCATCTT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

ZK121.1. External left primer: AAATTGATTTTCAATGCCGC. External right primer: TCCGATGTTCGTGTTGCTTA. Internal left primer: TTCAGAAGTGTCTTGGCACG. Internal right primer: CTTTGAAGGCATCTCAACCG. Internal WT amplicon: 1154 bp. Deletion size: 470 bp. Deletion left flank: CTTAGCAAATTCTACAGTAATTCTTTAAAT. Deletion right flank: ACACATAAATCGTGGTAGGTGTGAAAACCT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

Y113G7B.17. Identified by PCR, validated by CGH. External left primer: CAGGTACCTCTCCACACCGT. External right primer: TCCTGCGGACAAGAGAGATT. Internal left primer: TGGAGTACCGAGCAGAGTGA. Internal right primer: TGCTGTGAATATCGATTGGC. Internal WT amplicon: 2740 bp. Deletion size: 1236 bp. Deletion left flank: GTTTCAGTCAGAAAATCCGGTTTTTACTCG. Deletion right flank: TTGAGCATCGACTCGTAGAACAAGCAGTAT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

C14A4.13. Identified by PCR, validated by CGH. External left primer: CACCTTTCCCCATGAAAATG. External right primer: GTCGCCAATGAGACGAATTT. Internal left primer: GCAGCAGTAGTGGCAGTGAA. Internal right primer: TTTCCCGAAGAAGAACGATG. Internal WT amplicon: 2574 bp. Deletion size: 945 bp. Deletion left flank: ATGTTTGTAAGTTGAATCTTATCTAAAGCT. Deletion right flank: GCACCAGTAGCACATTCTCCACGGAAATCG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

ok3135. External left primer: TGCTCCGAATTTACTGACCC. External right primer: TCTCCCCAATTTGCTCATTC. Internal left primer: TGGCTTTGTTTCAGGTTTGA. Internal right primer: GCTCATAAACACCCGCATTT. Internal WT amplicon: 1224 bp. Deletion size: 523 bp. Deletion left flank: TTTGTTTCAGGTTTGATAGTGAATTTGACA. Deletion right flank: GTCTATTATTTCAATGCTTCTCTGTACATC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F56F3.1. Apparent homozygous lethal deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes), and ok3277 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: ATGGAACCACAGGTTGGTGT. External right primer: ATCAAGAAAGATCGATGGCG. Internal left primer: CACGGGACATCATCATCTTG. Internal right primer: GTGAGGAAACTGCTGGAGGA. Internal WT amplicon: 1110 bp. Deletion size: 568 bp. Deletion left flank: CTCTTGGTTGCTCTCTGGCGGGCTCTGACT. Deletion right flank: GGATCTTGCAATGGACTGACTTTTGAAGGT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

K09A9.5. Apparent homozygous lethal deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok3301 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: CATTCAAATCAGGGTGGAGG. External right primer: TGCCACTGAAAAGCTCATTG. Internal left primer: GCGCGTGTGGTCCTAATTT. Internal right primer: AAAATCTTCAACTCGGTCCAA. Internal WT amplicon: 1268 bp. Deletion size: 560 bp. Deletion left flank: TGTAAATTTAAAATTTTTTATATATAATAT. Deletion right flank: GCTCATCGATACGTTCTGGGAACTTGATGG. Insertion Sequence: CGATAC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F22F1.2. External left primer: ACAGGGGATTATGGGAGGAC. External right primer: TTCGCCACTTGTTCAATCTG. Internal left primer: ACTGGGCCTTGAGAGAATCA. Internal right primer: ATTTACACTGTGCACACCCG. Internal WT amplicon: 1206 bp. Deletion size: 594 bp. Deletion left flank: TTAGTCATTTTGTGTCCATCGATATCTTCC. Deletion right flank: CGCGAACACCATTTATCTCTTTGTGAATCA. Insertion Sequence: TA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F54G2.2. External left primer: AATCAGTTAAAGGGGTGGGG. External right primer: CAGCAACAAACTCAGCCAAA. Internal left primer: AATGGTGGGTGGTTGTCTGT. Internal right primer: TGTTCACACTTTAGATGTATTTCCG. Internal WT amplicon: 1153 bp. Deletion size: 625 bp. Deletion left flank: ACGAGATGGAAAGACCATCATCCTGCTTCT. Deletion right flank: CAGAGCTGAATTCCCATTTTTCCATATTCA. Insertion Sequence: T. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

C17G1.6. External left primer: GGCAGTTTGTCTTTTCACCC. External right primer: CAGAAGACATCGACAGGCAA. Internal left primer: TCTTGTGCTTGATGAGAGGAA. Internal right primer: CGTTCCGAAGGAGATGATGT. Internal WT amplicon: 1326 bp. Deletion size: 484 bp. Deletion left flank: GAATCTTTCTGTTGGTGGATGAACTTCCAA. Deletion right flank: GCTGTATCTGGAGCAACTGTCGTCGTCGGC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807