Caenorhabditis Genetics Center (CGC)

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Strain Species Genotype
WM159 C. elegans T23D8.7(tm1163) I. Show Description
JMC209 C. elegans alg-5(tor145[GFP::3xFLAG::alg-5]) I. Show Description
GFP and 3xFLAG tags inserted into endgonenous alg-5 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013
TAM2 C. elegans alg-5(ram2[GFP::3xFLAG + LoxP]) I. Show Description
alg-5(ram2[GFP::3xFLAG + LoxP]) is a deletion allele of alg-5. The coding region of the alg-5 genomic locus was replaced with GFP::3xFLAG using CRISPR-Cas9. Transgenic animals express GFP::3xFLAG under the control of alg-5 regulatory elements. GFP expression in the germline can be seen under a microscope. Reference: Nucleic Acids Res. 2017;45(15):9093-9107. doi:10.1093/nar/gkx536 PMID: 28645154