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Strain Species Genotype
BC13727 C. elegans dpy-5(e907) I; sEx13727. Show Description
sEx13727 [rCes y113g7b.17::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
DM7204 C. elegans pha-1(e2123) III; raEx204. Show Description
raEx204 [Y113G7B.17::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444.
RB2047 C. elegans prmt-1(ok2710) V. Show Description
Y113G7B.17. Homozygous. Outer Left Sequence: AACTACGAGTTTTGGGGGCT. Outer Right Sequence: TTTCGTGTGTGTTGGGTCTC. Inner Left Sequence: TTTCAGCCCAAAAATCGAAG. Inner Right Sequence: GGCCCTAAAAGTGGATTTCA. Inner Primer PCR Length: 1126 bp. Deletion Size: 532 bp. Deletion left flank: CGTAGAGACGAGCCTTGTCCGGGAACAACA. Deletion right flank: CTTCCCGTTTTCGGTACTCATTGTCCCGCT. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC2597 C. elegans Y113G7B.17(gk1117) V. Show Description
Y113G7B.17. Identified by PCR, validated by CGH. External left primer: CAGGTACCTCTCCACACCGT. External right primer: TCCTGCGGACAAGAGAGATT. Internal left primer: TGGAGTACCGAGCAGAGTGA. Internal right primer: TGCTGTGAATATCGATTGGC. Internal WT amplicon: 2740 bp. Deletion size: 1236 bp. Deletion left flank: GTTTCAGTCAGAAAATCCGGTTTTTACTCG. Deletion right flank: TTGAGCATCGACTCGTAGAACAAGCAGTAT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807